The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box

Liu, Yang; Zhao, Tong; Liu, Jing Mei; Liu, Weiqun; Liu, Qiang; Yan, Yong-Bin; Zhou, Hai

doi:10.1016/j.febslet.2006.01.048

Cited by 86 publications

(76 citation statements)

References 32 publications

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“…It was already known that the AP2/ERF domain recognizes DNA through the conserved arginine and tryptophan residues located in β-sheets (Allen et al, 1998). Likewise, Ala-37 in the ERF domain plays an important role in the stability of the ERF domain and in the binding of the DRE element or GCC-box to the DNA (Liu et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and phylogenetic analysis of the AP2/ERF gene superfamily in sweet orange (Citrus sinensis)

Ito¹,

Polido²,

Rampim³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Sweet orange (Citrus sinensis) plays an important role in the economy of more than 140 countries, but it is grown in areas with intermittent stressful soil and climatic conditions. The stress tolerance could be addressed by manipulating the ethylene response factor (ERF) transcription factors because they orchestrate plant responses to environmental stress. We performed an in silico study on the ERFs in the expressed sequence tag database of C. sinensis to identify potential genes that regulate plant responses to stress. We identified 108 putative genes encoding protein sequences of the AP2/ERF superfamily distributed within 10 groups of amino acid sequences. Ninety-one genes were assembled from the ERF family containing only one AP2/ ERF domain, 13 genes were assembled from the AP2 family containing two AP2/ERF domains, and four other genes were assembled from the RAV family containing one AP2/ERF domain and a B3 domain. Some conserved domains of the ERF family genes were disrupted into a few segments by introns. This irregular distribution of genes in the AP2/ ERF superfamily in different plant species could be a result of genomic losses or duplication events in a common ancestor. The in silico gene expression revealed that 67% of AP2/ERF genes are expressed in tissues with usual plant development, and 14% were expressed in stressed tissues. Because the AP2/ERF superfamily is expressed in an orchestrated way, it is possible that the manipulation of only one gene may result in changes in the whole plant function, which could result in more tolerant crops.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification and phylogenetic analysis of the AP2/ERF gene superfamily in sweet orange (Citrus sinensis)

Ito¹,

Polido²,

Rampim³

et al. 2014

Genet. Mol. Res.

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“…The drnl-2 allele has a base substitution from C to T at position +278, resulting in an A to V substitution at amino acid 93. This conserved A residue has recently been shown in Brassica napus ERF/AP2 proteins to be essential for DNA binding (Liu et al, 2006), suggesting that the drnl-2 AP2 domain is unable to bind target genes. For the phv 899_CO2 allele, the insertion is after nucleotide +84.…”

Section: Drn Drnl and Phv Mutantsmentioning

confidence: 99%

“…PAS domains have been reported to mediate protein-protein interactions (Taylor and Zhulin, 1999;Card et al, than drnl-1. The mutated Ala residue has been shown in Brassica napus ERF/AP2 proteins to be essential for DNA binding (Liu et al, 2006), suggesting that the mutated drnl-2 protein is unable to bind target genes. Importantly, the penetrance of hypophysis or cotyledon phenotypes significantly increased in drn-1 drnl-1 double mutants, thereby demonstrating a role for DRN and DRNL in both the apical and basal domain of the Arabidopsis embryo and that the genes act redundantly.…”

Section: Drn and Drnl Form Heterodimers With Class III Hd-zip Proteinmentioning

confidence: 99%

The AP2 transcription factors DORNRÖSCHEN and DORNRÖSCHEN-LIKE redundantly controlArabidopsisembryo patterning via interaction with PHAVOLUTA

et al. 2007

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DORNRÖSCHEN (DRN) (also known as ENHANCER OF SHOOT REGENERATION1; ESR1)and DRN-LIKE (DRNL; also known as ESR2) are two linked paralogues encoding AP2 domain-containing proteins. drn mutants show embryo cell patterning defects and, similarly to drnl mutants, disrupt cotyledon development at incomplete penetrance. drn drnl double mutants with weak or strong drnl alleles show more highly penetrant and extreme phenotypes, including a pin-like embryo without cotyledons, confirming a high degree of functional redundancy for the two genes in embryo patterning. Altered expression of PIN1::PIN1-GFP and DR5::GFP in drn mutant embryos places DRN upstream of auxin transport and response. A yeast two-hybrid screen with DRN followed by coimmunoprecipitation and bimolecular fluorescence complementation revealed PHAVOLUTA (PHV) to be a protein interaction partner in planta. drn phv double mutants show an increased penetrance of embryo cell division defects. DRNL can also interact with PHV and both DRN and DRNL can heterodimerise with additional members of the class III HD-ZIP family, PHABULOSA, REVOLUTA, CORONA and ATHB8. Interactions involve the PAS-like C-terminal regions of these proteins and the DRN/DRNL AP2 domain.

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“…A single amino acid substitution at Val14 by alanine or Glu19 by aspartic acid in the AP2 domain of DREB2A reduced the DNA binding activity and changed the DNA binding specificity of the protein (1). Liu et al reported that the GCC and DRE-binding ability of DREB1A and AtERF1 were reduced when Ala37 and Ala38 in the AP2 domain were mutated to Val (7).…”

Section: Introductionmentioning

confidence: 99%

Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution

Jin

Zhu²,

Peng³

et al. 2010

BMB Reports

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In this study, we cloned the ERF-B3 subfamily transcription factor gene BnaERF-B3-hy15 from Brassica napus L. Huyou15. This 600 bp gene encodes a 199 amino acid classic ethylene responsive factor (ERF), which shown no binding or very weak binding GCC box-binding activity by the yeast one-hybrid assay. We used gene shuffling and the yeast one-hybrid system to obtain three mutated sequences that can bind to the GCC box. Sequence analysis indicated that two residues, Gly156 in the AP2 domain and Phe62 at the N-terminal domain were mutated to arginine and serine, respectively. Changes of Gly156 to arginine and Phe62 to serine increased the GCCbinding activity of BnaERF-B3-hy15 and the alter of Gly156 to arginine changed the AP2-domain structure of BnaERF-B3-hy15. [BMB reports 2010; 43(8): 567-572]

show abstract

The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box

Cited by 86 publications

References 32 publications

Genome-wide identification and phylogenetic analysis of the AP2/ERF gene superfamily in sweet orange (Citrus sinensis)

Genome-wide identification and phylogenetic analysis of the AP2/ERF gene superfamily in sweet orange (Citrus sinensis)

The AP2 transcription factors DORNRÖSCHEN and DORNRÖSCHEN-LIKE redundantly controlArabidopsisembryo patterning via interaction with PHAVOLUTA

Optimizing the binding activity of the AP2/ERF transcription factor with the GCC box element from Brassica napus by directed evolution

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