The contribution of planar chromatography to food analysis

Abstract

Food generally enjoys public confidence; food analysis, however, is a growing and challenging sector. Analytical methods must keep pace with the continuously changing attitudes and preferences of consumers to ensure product quality. Planar chromatography, a very flexible technique for rapidly changing issues, can assist food analysts. But few users of planar chromatography (approx. 10%) use high-performance thin-layer chromatography (HPTLC). Use of appropriate instrumentation will improve the precision of the method. There is therefore, much potential to increase the efficacy and attraction of planar chromatographic applications in food analysis. Validated HPTLC methods fulfill the requirements for reliable food analysis in many fields. Planar chromatography can support food analysis by simultaneous determination of analytes with different detection principles, for example point of care analysis by digital imaging and effect-directed assays via bioactivitybased detection. Parallel analyses under identical conditions or the targeted use of mass spectrometry contributes to its cost-effectiveness. Planar chromatography is an optimum tool for national and international standards to keep analysis inexpensive -especially nowadays.

Milestones, Core Concepts, and Contrasts

“…The thin-layer format provides a better arrangement for high sample throughput, flexible detection strategies, and a greater tolerance of samples with a high-matrix burden [2,16]. The thin-layer format provides a better arrangement for high sample throughput, flexible detection strategies, and a greater tolerance of samples with a high-matrix burden [2,16].…”

“…This is not a concern for thin-layer chromatography because reuse of the stationary phase is not planned, and therefore, in the initial phases of a study designed to gain an understanding of the properties of the sample matrix on the analysis, or to facilitate the analysis of samples with minimal sample preparation, then thin-layer chromatography is the method of choice [2,16]. On account of their higher cost columns for HPLC are used to analyze multiple samples.…”

A Unifying Review of Bioassay-Guided Fractionation, Effect-Directed Analysis and Related Techniques

“…Therefore, effect-related analysis of such products may be particularly relevant. Some concepts based on thin-layer chromatography have been presented by Morlock and Schwack [ 151 ]. The same group also showed an innovative multienzyme inhibition system for the analysis of toxic insecticides in water or juice [ 152 ].…”

Serum uric acid concentration due to Gnetum gnemon chip supplementation and quality changes analyses based on its chemical constituents in post-frying process

“…Furthermore, an effective and efficient method for the quantification of chemical marker to estimate active bimolecular content is fundamental to precisely prepare the dose or conduct material standardizations. Densitometry, also called as high‐performance liquid chromatography (HPTLC), is a simple and convenient quantification procedure to target one or two chemical markers in a single or sample mixtures in raw material or final food product (Morlock & Schwack, ; Rohn, ; Sherma, ; Zhao & Shah, ). The stability of a material can be examined based on an antioxidant activity and in comparison to the intact material (Becker, Nissen, & Skibsted, ).…”