The determination of nucleic acids in biological materials: an amendment

Hutchison, W. C.; Munro, Hamish N.

doi:10.1039/an9628700303

Cited by 17 publications

(14 citation statements)

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“…Tissue samples to be analyzed for RNA, DNA, and collagen were rapidly frozen in liquid nitrogen and stored at -20°C. Determinations of RNA and DNA were done using E. coli t-RNA and calf thymus DNA standards and followed the respective procedures of Hutchinson and Munro [8] and Schneider [9]. Collagen was measured according to the method of Kivirikko et a1 [lo].…”

Section: Methodsmentioning

confidence: 99%

Hormonal control of accessory sex organ fibromuscular stroma

Mariotti

Mawhinney

1981

The Prostate

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Using the surgically separated epithelium and muscle preparation of the guinea pig seminal vesicle, quantitative determinations were made of the hormonal sensitivities of three components of male accessory sex organ fibromuscular stroma. The parameters measured were epithelial collagen, muscle tissue cell number, and muscle tissue collagen. Epithelial collagen increased approximately tenfold between one week of age and adulthood. This growth was completely prevented by castration and was sustained at normal levels of maintenance of prepubertal castrates with dihydrotestosterone (DHT). Castration of adult animals reduced epithelial collagen 40%; such reductions were prevented by DHT treatment. Estrogen treatment of adult castrates, either alone or in combination with DHT, had no anabolic effect on the epithelium. In all of these studies, changes in epithelial collagen correlated with changes in epithelial wet weight, DNA content, and RNA/DNA. From 1 week of age to adulthood, growth of seminal vesicle muscle involved marked increases in wet weight, DNA content, RNA/DNA, and collagen content. Increments in all parameters were prevented by prepubertal castration and were sustained at normal levels in castrates by DHT treatment. However, in adult animals, muscle DNA content and collagen levels were insensitive to castration and androgen replacement; only tissue weight and cell size (RNA/DNA) were androgen sensitive. Estrogen treatment of adult castrates caused supranormal increases in muscle DNA and collagen; changes in collagen were irreversible. The effects of simultaneous treatment of DHT and estrogen did not differ from those of DHT alone. Therefore, normal postnatal development of the stromal components, epithelial collagen, muscle cells, and muscle collagen was androgen dependent, but in adult animals, only epithelial collagen retained androgenic sensitivity. Estrogen induced supranormal and irreversible growths in the muscle.

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Section: Methodsmentioning

confidence: 99%

Hormonal control of accessory sex organ fibromuscular stroma

Mariotti

Mawhinney

1981

The Prostate

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“…The livers were excised, weighed and analyzed for deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) according to the modifications of the Schmidt-Thannhauser procedure suggested by Hutchison and Munro (1961). The daily injections of vitamin A were administered for 23 days as follows: group 1, 150 IU's (controls); group 2, 25,000; group 3, 50,000; and group 4, 100,000 IU's of vitamin A.…”

Section: Methodsmentioning

confidence: 99%

Vitamin A Toxicity and Its Effect on Liver Nucleic Acids, Protein and Lipids in the Chick

Squibb

1963

Poultry Science

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“…Total nucleic acid was assayed by a modification of the Schneider procedure (1945) as described by Hutchison and Munro (1961). Samples were mixed with cold, 10% trichloroacetic acid followed by pelleting.…”

Section: Nucleic Acidmentioning

confidence: 99%

Myelin subfractions isolated from mouse brain. Studies of normal mice during development, quaking mutants, and three brain regions

et al. 1977

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Myelin isolated from three areas of mouse brain, from whole brain at several ages in normal mice, and from whole brain of adult quaking mutant mice was separated into seven bands and a pellet on discontinuous density gradients using 0.32, 0.45, 0.55, 0.60, 0.70, 0.75 and 0.85 M sucrose. The distribution of myelin in the subfractions was independent of homogenization and shocking conditions employed to isolate the myelin preparations, but was related to the type of myelin applied to the gradient. Compared to myelin isolated from older animals, myelin isolated from 18-24 day old mice displayed a distribution pattern with greater proportions of material banding at lesser sucrose densities. Similarly, myelin obtained from hindbrain contained proportionately more material layering at lesser sucrose densities compared to myelin isolated from cerebral cortex. Myelin subfraction patterns observed for 8-12 day old control mice and quaking mutants were unlike each other or any other myelin preparation examined. In the 18-90 days old animals, the markers studied were not uniformly distributed among the myelin subfractions. The pellet and the layer banding at the 0.75/0.85 M sucrose interface contained the highest specific concentrations of sialic acid, nucleic acid, and total adenosine triphosphatase activity. In contrast, the specific activity of 2',3'-cyclicnucleotide-3'-phosphohydrolase was lowest in the pellet as well as the three bands obtained above 0.60 M sucrose and was highest in the fraction banding at the 0.65/0.70 M sucrose interface. The results obtained were not consistent with an artifactual origin of the myelin subfractions, but instead suggested that the subfraction have physiological significance. One explanation for the different banding patterns observed between young and mature myelin may be the different amount of myelin in various brain regions during development.

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The determination of nucleic acids in biological materials: an amendment

Cited by 17 publications

References 0 publications

Hormonal control of accessory sex organ fibromuscular stroma

Hormonal control of accessory sex organ fibromuscular stroma

Vitamin A Toxicity and Its Effect on Liver Nucleic Acids, Protein and Lipids in the Chick

Myelin subfractions isolated from mouse brain. Studies of normal mice during development, quaking mutants, and three brain regions

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