The Effect of Different Levels of Lycopene in Egg Yolk Citrate Extenders on Post-Thawed Semen Qualities of Belgian Blue Crossbreed Bull

Abstract

As many as seven 500-kg-Belgian-Blue-Crossbreed-bulls aged four years old were kept in a mooring cage and served with forage and concentrate (CP 16%) at 10% and 3% to the body weight, respectively. There were also added some egg yolk citrate diluent of 2.9% (v/v), Sodium citrate dihydrate buffer, 100 mL of distilled water, and an egg yolk mixture to a proportion of 20% egg yolk 8% (v/v) glycerol (Merck, Germany), penicillin (1,000 IU/mL), and streptomycin (1,000 mg/mL). Sperm was diluted to 50 million/mL and treated with lycopene extract levels of 0% (control), 1%, 2%, 3%, and 4% in a diluent, equilibrated at the temperature of 5°C for 4 hours, packed in 0.25 ml straw, and refrozen into liquid nitrogen vapor about 5 cm above the liquid nitrogen surface for 10 minutes. Freezing was run by immersing the forage in liquid nitrogen for 24 hours before the post-thawing examination. Results showed that spermatozoa diluted with egg yolk citrate supplemented with 3% lycopene (P3) had better quality than that found in the other four treatments, namely motility before freezing (70.86%) and post thawing (53.00%); viability before freezing (76.00%) and post thawing (60. 14%); plasma membrane integrity before freezing (71.29%) and post thawing (59.86%); and also showed the lowest abnormality level before freezing and post-thawing, (17.29%) and (24.14%), respectively.

Protection of Myrmecodia Extract in Tris Diluent on PO Bull Sperm Quality During Freezing

“…According to Ros Santaella and Pintus (2021), using natural plant extracts as additional diluents can protect sperm during cooling. Several extracts have high antioxidant content, such as green tea extract (Prastiya et al, 2023), soybean extract (Ratnawati et al, 2023), Lycopene (Bintara et al, 2023), red fruit oil (Nurcholis et al, 2021), Eurycoma longifolia extract (Baiee et al, 2018). Myrmecodia contains secondary metabolite compounds in the form of flavonoids and tannins which can inhibit dangerous cell proliferation through its antioxidant activity (Imaniar et al, 2022).…”