The effect of ethanol extract of myrmecodia pendens to tgf-β1 expression and osteoblast cells after tooth extraction (the experimental research on cavia cobaya)

Abstract

Objective: Myrmecodia pendens is often used as a traditional medicine to treat various diseases. A previous study showed that ethanol extract of sarang semut plant (hypnophytum furmicarum jack) 4.65 mg was effective in fastening establishment of socket granulation tissue after tooth extraction; likewise, the myrmecodia pendens at 3% also fastens the healing process of soft-tissue wound after tooth extraction. The aim of this study is to assess the effect of ethanol extract of 10% myrmecodia pendens on the expression of TGF-β1 and determine the number of osteoblasts that remain after tooth extraction. Material and Methods:This study used 24 male marmots divided into 2 groups. All of the groups were extracted in the 1st left-incisor mandibula and 0.1 ml of 0.5% CMC was applied into the socket control group. The treatment group was given 0.1 ml ethanol extract of myrmecodia pendens 10% dissolved with CMC 0.5%. On day 3, 7, 14 and 21, marmots were decapitated and histopathologic preparations were observed to study the expression of TGF-β1 and the number of osteoblast cells. Data were analyzed using Kruskall-Wallis test with a probability p < 0.05. Results: Based on the Kruskal-Wallis test, there was a significant difference in the TGF-β1 expression and the number of osteoblast cells after the administration of ethanol extract of myrmecodia pendens between the control group and treatment group treatment (p < 0.05). Conclusion:The ethanol extract of myrmecodia pendans can fasten the healing of the wound resulting from tooth extraction; it accelerates the healing process by increasing the expression of TGF-β1 and the number of osteoblast cells.Keywords: Myrmecodia pendens, Osteoblasts, TGF-β1, Tooth extraction Cite this Article: Ismardianita E, Nasrul E, Yanwirasti, Hemiawati M. 2017. Effect of ethanol extract of myrmecodia pendens on TGF-β1 expression and osteoblast cells after tooth extraction (experimental research on cavia cobaya). Journal of Dentomaxillofacial Science 2(3): 150-154.

The Effect of Application Bovine Amniotic Membrane on Osteoblasts, Osteocytes, and Collagen

“…This decrease in collagen thickness occurs because collagen fibers begin to be replaced with bone matrix. 14 The results in Table 3 are in line with previous studies, which said that there was a rapid increase in the level of collagen thickness until the 21 st day, and the thickness would continue to decrease until the 90 th day. 14,29 The value in the control group was not too high because this group did not get any medication to support the healing process.…”

“…14 The results in Table 3 are in line with previous studies, which said that there was a rapid increase in the level of collagen thickness until the 21 st day, and the thickness would continue to decrease until the 90 th day. 14,29 The value in the control group was not too high because this group did not get any medication to support the healing process. However, it remained a normal inflammatory phase, resulting in low collagen stimulation.…”

“…This is in line with previous research, which said that the thickness of collagen would continue to increase from the 3 rd day after tooth extraction to the 14 th day with a denser collagen image. 28,14 However, collagen thickness decreased on the 28 th day in each group. This decrease in collagen thickness occurs because collagen fibers begin to be replaced with bone matrix.…”

“…Similarly, collagen also seems to decrease after the 14 th day and will continue to decrease until the 28 th day. 14,15 Histological analysis can be used as an option for examining a tissue. 16 This method can be used to observe bone microarchitecture, showing the mineral components, bone cells, and physiological processes of bone formation and resorption.…”

Changes in osteoblast and osteoclast cell count after moringa oleifera leaf extract administration during orthodontic tooth movement

“…Flavonoid can manage cell function by stimulating TGF-β production, whereas TGF-β induces proliferation and migration of osteoblast. 19 The increase of osteoblastic proliferation and activation may increase the amount of osteoblast, which further increase the expression of osteoprotegerin (OPG). 4 An increase of OPG is very influential to the osteoclast activation, since OPG is the decoy receptor for Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL), by inhibiting the interaction between RANKL and Receptor Activator of Nuclear Factor Kappa-B (RANK) in precursor osteoclast cell membrane, thus inhibit the osteoclastogenesis.…”