The effect of initial irrigation with two different sodium hypochlorite concentrations on the erosion of instrumented radicular dentin

Effects of Concentrations and Exposure Times of Sodium Hypochlorite on Dentin Deproteination: Attenuated Total Reflection Fourier Transform Infrared Spectroscopy Study

“…Then, the deproteination process of NaOCl reverts to the hydroxyapatite-encapsulated collagen and shows little change over a period of several minutes (12). Other studies showed that the apatite:collagen ratio and exposure time (10-240 minutes) of NaOCl (1.3% and 5.25%) were linearly related (13). On the other hand, our study confirms that the NaOCl treatment leads to the concentrationdependent collagen depletion.…”

“…The time-dependent effects of a high-concentration NaOCl (12%) on dentin deproteination from 0 to 48 hours at time intervals of 0.5 hours had been reported (12). Linear relationships were found to exist between exposure time (10 to 240 minutes) of NaOCl (1.3% and 5.25%) and ''apatite/collagen ratio'' of dentin powders (13). However, to our knowledge, there has been no direct quantitative study about the effects of the low concentration (0.5%-2.25%) and the short exposure time (1-10 minutes) of NaOCl on dentin deproteination.…”

“…After baseline correction and normalization, the effect of NaOCl on collagen depletion was evaluated using the collagen and apatite ratio (the ratio of absorbance of amide I peak to phosphate v 3 peak) (12)(13)(14)(15). Smaller ratio (amide:phosphate) values corresponded to a greater extent of dentin deproteination.…”

Effect of chemical interaction on the bonding strengths of self-etching adhesives to deproteinised dentine

“…23 These bands are directly related to polypeptide chains of type I collagen component in dentine. 16,24 The spectra suggested a clear weakening of the peaks at Amide I, Amide II and Amide III after NaOCl treatment.…”

“…15 For instance, sodium hypochlorite (NaOCl), which is the most common endodontic irrigant for chemical debridement of the root canal space, can deproteinise demineralised as well as mineralised dentine. 16,17 Nevertheless, the effect of NaOCl collagen deproteinisation on dentine bonding remains controversial. Lai et al found that NaOCl deproteinisation adversely lowered the bonding strength of dentine because of its strong oxidising properties.…”