The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor‐20 on Parkinson’s Disease

Abstract

Cell therapy is a potential therapeutic approach for Parkinson's disease (PD). Mesenchymal stem cells derived from the human umbilical cord (hUC-MSCs) give priority to PD patients because of multiple advantages. The appropriate gene transduction of hUC-MSC before transplantation is a promising procedure for cell therapy. Fibroblast growth factor-20 (FGF-20) has been shown to protect dopaminergic neurons against a range of toxic insults in vitro. In this study, the hUC-MSCs were gene transduced with FGF-20, and then we transplanted them into the PD mice model. The results showed that MSC-FGF-20 treatment obviously improved the behavior of PD, accompanied by the increase of tyrosine carboxylase- (TH-) positive cell and dopamine (DA). Furtherly, immunohistochemistry disclosed that MSC-FGF-20 obviously promoted the degradation of nuclear factor-κB (NF-κB), a transcription factor that controls genes encoding proinflammatory cytokines, highly expressed in the nigrostriatal dopaminergic regions in PD patients. Therefore, MSC-FGF-20 has a potential for improving PD, closely related to the degradation of NF-κB.

Human Dental Pulp‐Derived Mesenchymal Stem Cell Potential to Differentiate into Smooth Muscle‐Like Cells In Vitro

“…Mesenchymal stem cells (MSCs) are the specialized cells which can be easily isolated from different tissues of the body and can self-replicate, propagated in vitro to desired cell numbers, having multidifferentiation potential and immunomodulatory features and showing better homing abilities when transplanted in vivo [ 1 ]. Due to such peculiar characteristics, MSCs have been successfully used to treat neurodegenerative, cardiac, pancreatic, and hepatic disorders [ 2 – 4 ]. However, the selection of a suitable MSC source is also highly important as different source-derived stem cells have been shown to display varied stemness and differentiation abilities [ 5 – 7 ].…”

Signal Factors Secreted by 2D and Spheroid Mesenchymal Stem Cells and by Cocultures of Mesenchymal Stem Cells Derived Microvesicles and Retinal Photoreceptor Neurons

“…For these reasons, MSCs have great therapeutic potential in disease treatment, as demonstrated by results from multiple experimental and clinical studies [ 4 – 6 ]. In addition to their multidifferentiation potential, MSCs are well known for their abilities to secrete paracrine factors and to modulate inflammation and immunity [ 7 , 8 ]. Interestingly, therapeutic effects are frequently reached without convincing evidence of cell differentiation or engraftment in vivo [ 9 ].…”

FGF, Mechanism of Action, Role in Parkinson’s Disease, and Therapeutics

“…Furthermore, human umbilical cord mesenchymal stem cells (hUC-MSCs) derived from the human umbilical cord also have great potential in the treatment of PD. Introduction of FGF-20 gene into hUC-MSCs and transplantation into a mouse model of PD significantly improved mouse behavior, accompanied by an increase in tyrosine hydroxylase-positive cells and dopaminergic neurons, which may be related to MSC-FGF-20 promoting the degradation of the transcription factor NF-κB in the nigrostriatal dopaminergic system ( Jinfeng et al, 2016 ).…”