The Effect of Proctodeal Gland Foam, Diluent and Dimethylacetamide Addition on Morphology and Fertilising Ability of Japanese Quail (<I>Coturnix japonica</I>) Spermatozoa

Abstract

The e#ect of proctodeal gland foam addition to fresh net or diluted semen supplemented with (DMA) dimethylacetamide, on morphology and fertilising potency of quail spermatozoa was evaluated.Every stage of quail semen preparation caused an increase in sperm deformations. The addition of foam, diluent and DMA decreased the number of morphologically intact spermatozoa by ,2.-percent points, in relation to the fresh semen.Foam addition to the fresh semen allowed to obtain 3+..ῌ fertility, which was similar to results obtained during natural mating (3-.*ῌ), but in diluted and DMA supplemented semen, equilibrated for / minutes at .῍, the foam had a detrimental e#ect on fertilising ability of spermatozoa.

Production of Live Offspring from Testicular Tissue Cryopreserved by Vitrification Procedures in Japanese Quail (Coturnix japonica)1

“…Cheng et al [28] found that the deposition of foam into the female proctodeum during copulation had a positive effect on the percentage of females fertilized and the duration of fertility. However, Chełmońska et al [29] reported that mixing foam with ejaculated semen diluted with extender and dimethylacetamide was detrimental to sperm morphology and fertility. In the present study, foam was added to the testicular exudate, and the mixture was deposited in the magnum.…”

Relationship between semen quality and level of heterozygosity on the model of endangered population of Western capercaillie

“…Taking into consideration the essence of the correct sperm structure in the fertilization process, in our experiments dealing with assessment of male reproductive potency we used to evaluate sperm morphology method basing on the nigrosine‐eosin histological smears and 1250× microscopic magnification (Chełmońska et al, 2006; Klimowicz et al, 2005; Kowalczyk et al, 2012; Kowalczyk & Łukaszewicz, 2015; Łukaszewicz et al, 2008, 2011b) under a light microscope (Nikon Eclipse E 100, Tokyo, Japan). The formulation of the stain was as follows: one part of 4% eosin solution (4 g eosin dissolved in 96 mL of 2.9% sodium citrate) and three parts of 8% nigrosine solution (8 g nigrosine dissolved in 92 mL of 2.9% sodium citrate) (Głód, 1976).…”