The effect of red-fleshed pitaya (Hylocereus polyrhizus) on heat shock protein 70 and cortisol expression in strenuous exercise induced rats

Abstract

Background: Oxidative stress from exercise can contribute to damaging cells, increasing heat shock protein 70 (HSP70) and suppressing the immune system in the body. This research aimed to determine the antioxidant potential of red-fleshed pitaya extract on HSP70 and cortisol expression in rats which were subjected to strenuous exercise. Methods: The subjects of this research were 32 Sprague Dawley male rats, aged 3 months, with an average weight of 200 g. Red-fleshed pitaya extract was obtained from methanol extraction process; a maceration technique was performed and the extract was concentrated using an air-drying method. Rats were randomly divided into four groups. Group 1 were subjected to strenuous exercise and treated with distilled water only; while Groups 2, 3 and 4 were subjected to strenuous exercise and treated with 100 mg/kg body weight, 200 mg/kg body weight and 300 mg/kg body weight of red-fleshed pitaya extract, respectively. Strenuous exercises in rats was performed by intense swimming of 20 min/day, 3 days a week for 3 weeks. HSP70 expression and cortisol were measured with Enzyme-Linked Immune Sorbent Assay (ELISA) method. Results: There was a significant reduction of HSP70 (p=0.000) and cortisol expression (p=0.000) between the groups. Also, there was a significant difference in the average decreasing of HSP70 expression between group 4 and either groups 1 or 2 (p=0.000). However, a significant difference between groups 4 and 3 was not observed (p=0.813). Lastly, a significant difference was found in the average decrease of cortisol expression between groups 4 and 1 (p=0.000), 2 (p=0.000), and 3 (p=0.000) respectively. Conclusion: Red-fleshed pitaya is potential to be utilized as antioxidant to decrease the HSP70 and cortisol expression.

The effect of red-fleshed pitaya (Hylocereus polyrhizus) on heat shock protein 70 and cortisol expression in strenuous exercise induced rats

“…Underlying data Open Science Framework: The effect of red-fleshed pitaya (Hylocereus polyrhizus) on heat shock protein 70 and cortisol expression in strenuous exercise induced rats, https://doi. org/10.17605/OSF.IO/MGX4K 41 Data are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).…”

“…Open Science Framework: The effect of red-fleshed pitaya ( Hylocereus polyrhizus ) on heat shock protein 70 and cortisol expression in strenuous exercise induced rats, https://doi.org/10.17605/OSF.IO/MGX4K 41 …”

Regular Exercise of Moderate Intensity and Lemon Peel Essential Oil as Immunomodulator during Covid-19

“…The findings of this study are consistent with earlier research indicating that red dragon fruit possesses antioxidant capacity. The red dragon fruit metabolite compound containing flavonoids can neutralize free radicals formed by physical exercise to prevent oxidative stress [36]. Decreased CRP levels occurred in both groups due to regular and regular physical exercise having an overall anti-inflammatory influence mediated by several pathways, including increased control of inflammatory signaling pathways, stimulating myokine muscles for il-1ra and IL-10 production [37].…”

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