The effect of testosterone on the rate of oxygen consumption by prostate tissue

Abstract

Aim. To study the effect of testosterone on the rate of oxygen consumption by rodent prostate homogenate. Methods. The study included 30 healthy old white outbred male rats divided into two groups with 15 animals in each group. The rats of the first group were administered the application of 1 % testosterone-containing gel Androgel before the operation. The rats of the second group received no testosterone. Under anesthesia prostatectomy was performed. Homogenate was immediately prepared from each prostate. Further every homogenate was placed into 250 ml vial to determine the rate of oxygen consumption. Then the device measuring the concentration of oxygen dissolved in water was placed into the vial, and the air-tightness was created. Each vial was put into the thermostat for 30 minutes at 36.6 ˚C. Then the measurement of the concentration of O2 dissolved in the water was performed. Results. Application of transdermal gel with 1 % testosterone was found to cause increase of oxygen consumption by prostate tissue. This fact can explain why the clinical effectiveness of testosterone is individual to each patient with benign prostatic hyperplasia (BPH) and chronic prostatitis (CP): oxygen supply to the prostate is different in each patient with BPH and CP. So not in every patient the oxygen-transporting system is capable of supplying prostate tissues with the amount of oxygen according to increasing demand of the organ on testosterone administration. Conclusion. Testosterone increases the rate of oxygen consumption by prostate tissue.

Is the concentration of potassium in ejaculate capable to be the predictor of clinical efficacy of tadalafil in men with chronic prostatitis 3 B?

“…There is a reason to believe that there is a material, materialistic factor that fundamentally influences the clinical efficacy of tadalafil in CP3B [2,3,12].…”

“…The complexity and multifactorial nature of the etiology and pathogenesis, the scarcity of detected pathological changes make researchers expand the diagnostics through procedures that go beyond "traditional" methods. This highlights the fact that CP3B is an interdisciplinary problem [3]. In recent years, the volume of scientific data has been growing, convincingly testifying to the important role of ischemic processes in the pathogenesis of CP3B [4,5].…”

“…Today, the mechanism of action of tadalafil on prostate tissue is not clearly understood [6]. The results of prescribing tadalafil to patients with CP3B are contradictory [3,7,8].…”

Comparative study of the influence of tadalafil and doxazosin on the rate of oxygen consumption by prostate tissue in Sprague-Dawley rats and in white outbred rats. Possible clinical consequences of unstudied pharmacological effect in men with lower urinary tract symptoms

“…In 2016, we were the first to detect that the α 1adrenoblocker doxazosin increases the rate of oxygen consumption by the tissue of prostate in white outbred rats (WOR) [3].…”

“…An amazing fact that deserves further comprehensive study, we consider the ability of tadalafil to increase the rate of oxygen consumption by prostate tissue in WOR [4,5].…”