The Effects of<i>Eucheuma cottonii</i>on Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM<sub>10</sub>) Coal Dust

Kania, Nia; Mayangsari, Elly; Setiawan, Bambang; Nugrahenny, Dian; Tony, Frans; Wahyuni, Endang Sri; Widodo, M. Aris

doi:10.1155/2013/528146

Cited by 12 publications

(4 citation statements)

References 26 publications

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“…Similarly, the protein carbonyl content in the lung tissue did not change following exposure to PM extracts (Figure 3C). The lipid peroxidation finding differs from the prominent increase in peroxidative damage observed following exposure of mice' lungs to low doses (15 μg) urban PM collected in Buenos Aires, 5 or exposure of rat lungs to coal dust PM10, 43 and in RAW 264.7 cells exposed to welding fumes (which contains a complex mix of metal oxide particles). 44 Collectively, these data indicate that exposure to PM containing high concentrations of metals induces lung lipids damage.…”

Section: Environmental Science and Technologycontrasting

confidence: 71%

Single Exposure to near Roadway Particulate Matter Leads to Confined Inflammatory and Defense Responses: Possible Role of Metals

Pardo

Shafer

Rudich

et al. 2015

Environ. Sci. Technol.

103

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Inhalation of traffic-associated atmospheric particulate matter (PM2.5) is recognized as a significant health risk. In this study, we focused on a single ("subclinical response") exposure to water-soluble extracts from PM collected at a roadside site in a major European city to elucidate potential components that drive pulmonary inflammatory, oxidative, and defense mechanisms and their systemic impacts. Intratracheal instillation (IT) of the aqueous extracts induced a 24 h inflammatory response characterized by increased broncho-alveolar lavage fluid (BALF) cells and cytokines (IL-6 and TNF-α), increased reactive oxygen species production, but insignificant lipids and proteins oxidation adducts in mouse lungs. This local response was largely self-resolved by 48 h, suggesting that it could represent a subclinical response to everyday-level exposure. Removal of soluble metals by chelation markedly diminished the pulmonary PM-mediated response. An artificial metal solution (MS) recapitulated the PM extract response. The self-resolving nature of the response is associated with activating defense mechanisms (increased levels of catalase and glutathione peroxidase expression), observed with both PM extract and MS. In conclusion, metals present in PM collected near roadways are largely responsible for the observed transient local pulmonary inflammation and oxidative stress. Simultaneous activation of the antioxidant defense response may protect against oxidative damage.

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Section: Environmental Science and Technologycontrasting

confidence: 71%

Single Exposure to near Roadway Particulate Matter Leads to Confined Inflammatory and Defense Responses: Possible Role of Metals

Pardo

Shafer

Rudich

et al. 2015

Environ. Sci. Technol.

103

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“…Eucheuma is a type of edible seaweed, which has expectorant, antitussive, antipyretic, and detoxification effects. The ethanol extract of Eucheuma has been shown to alleviate oxidative stress in the lungs of rats chronically exposed to coal dust (Kania et al, 2013; Saputri et al, 2014). The extract may exert a protective effect on lead‐induced liver injury in rats through its antioxidant activity (Wardani, Farida, Andayani, Kuntoro, & Sudjarwo, 2017).…”

Section: Discussionmentioning

confidence: 99%

Acute toxicological study: EZY ‐1 with potent therapeutic effects of idiopathic pulmonary fibrosis and its mechanisms

Huang

et al. 2022

Journal of Food Biochemistry

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EZY‐1 is an antifibrosis peptide purified from Eucheuma. In this study, we explored the acute toxicology of EZY‐1 and the signaling pathways involved in its antifibrotic role. The mouse model of pulmonary fibrosis was induced by bleomycin. Pathological changes in lung tissue could be effectively inhibited by EZY‐1. Acute toxicity and cell proliferation tests indicated that EZY‐1 had no apparent toxicity to mice and cells. We identified proteins that could bind directly to EZY‐1 in vitro on the basis of liquid chromatography–tandem mass spectrometry and bioinformatics analysis. EZY‐1 inhibited pulmonary fibrosis via Wnt/β‐catenin, transforming growth factor (TGF)‐β/Smad, phosphoinositide 3‐kinase/protein kinase B/ mammalian target of rapamycin, and activator of transcription 3 and Janus kinase 2/signal transducer pathways. A transwell micropore experiment showed that EZY‐1 could inhibit cell migration and invasion. Western blotting analysis on transforming growth factor‐β1 (TGF‐β1)‐induced A549 pulmonary fibrosis cell model suggested that EZY‐1 could downregulate p‐Smad3 (Ser423/Ser425), Smad4, β‐catenin, vimentin, and N‐cadherin expression. ELISA showed that EZY‐1 could inhibit collagen‐I secretion. EZY‐1 alleviated idiopathic pulmonary fibrosis (IPF) through regulating TGF‐β/Smad pathways, epithelial‐mesenchymal transition processes, and collagen secretion, which provides a potential foundation for theoretical development of EZY‐1 as a potential drug against IPF. Practical applications We isolated a new 16‐amino‐acid peptide derived from the polypeptide extract of Eucheuma, named EZY‐1. In vitro and in vivo assays show peptide EZY‐1 is safe. The EZY‐1 peptide alleviates IPF at lower doses than pirfenidone. EZY‐1 alleviated idiopathic pulmonary fibrosis (IPF) through regulating TGF‐β/Smad pathways, epithelial‐mesenchymal transition (EMT) processes, and collagen secretion, which provides a theoretical basis for the development of EZY‐1 as a potential drug against IPF.

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“…[1][2][3] Marine resources have recently become increasingly intriguing sources of new drugs and healthy foods. 4 Many people consume fresh and dried seaweed, particularly those living in coastal areas. 5 Although, some of the properties of algae have been investigated, many remain unexplored.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antibacterial Activities of the Tropical Red Alga Eucheuma spinosum: In Silico Study

Damongilala

Dotulong

Apriyanti

et al. 2023

Natural Product Communications

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Recently, numerous studies have focused on characterizing the biochemical activities of new natural marine resources as functional foods. Eucheuma spinosum J. Agardh., family Solieraceae, is a potential functional food that is widely used as a natural food source and contains bioactive components. This study aims to identify the bioactivity, drug-likeness, and pharmacokinetics of 3-(3-methoxyphenyl) propanal isolated from E. spinosum, and to discover the molecular interactions between the ligand, 3-(3-methoxyphenyl) propanal, and various antioxidant and antibacterial receptors. The bioactivity of 3-(3-methoxyphenyl) propanal was analyzed using computational Prediction of Activity Spectra for Substance (PASS) analysis. The drug-likeness was examined based on Lipinski's rule and other parameters. The pharmacokinetics of 3-(3-methoxyphenyl) propanal were predicted, and the molecular interactions between the receptors governing the antioxidant and antibacterial activity and 3-(3-methoxyphenyl) propanal were determined using the molecular docking method and molecular dynamics simulation. Based on the PASS analysis, 3-(3-methoxyphenyl) propanal was predicted to inhibit JAK2 expression, which contributes to apoptosis, with a probability of a molecule to be active (Pa) values of 0.733 and 0.508. Additionally, 3-(3-methoxyphenyl) propanal is an oxygen scavenger (Pa 0.408) and antimutagenic (Pa 0.419). Based on the drug-likeness and pharmacokinetic analyses, 3-(3-methoxyphenyl) propanal meets the requirements for an oral drug. Molecular docking revealed that binding affinity of 3-(3-methoxyphenyl) propanal for superoxide dismutase, tyrosinase, glutathione peroxidase, UDP-N-acetylmuramate, and penicillin-binding protein was −5.5, −6.1, −4.2, −6.2, and −4.9 kcal mol −1, respectively. Moreover, according to molecular dynamics simulation, propanal 3-(3-methoxyphenyl) propanal has stronger interaction with tyrosinase than the positive control. Based on PASS analysis and molecular docking results, 3-(3-methoxyphenyl) propanal exhibited promising potential as an antioxidant.

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The Effects ofEucheuma cottoniion Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM₁₀) Coal Dust

Cited by 12 publications

References 26 publications

Single Exposure to near Roadway Particulate Matter Leads to Confined Inflammatory and Defense Responses: Possible Role of Metals

Single Exposure to near Roadway Particulate Matter Leads to Confined Inflammatory and Defense Responses: Possible Role of Metals

Acute toxicological study: EZY ‐1 with potent therapeutic effects of idiopathic pulmonary fibrosis and its mechanisms

Antioxidant and Antibacterial Activities of the Tropical Red Alga Eucheuma spinosum: In Silico Study

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The Effects ofEucheuma cottoniion Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM10) Coal Dust

Cited by 12 publications

References 26 publications

Single Exposure to near Roadway Particulate Matter Leads to Confined Inflammatory and Defense Responses: Possible Role of Metals

Single Exposure to near Roadway Particulate Matter Leads to Confined Inflammatory and Defense Responses: Possible Role of Metals

Acute toxicological study: EZY ‐1 with potent therapeutic effects of idiopathic pulmonary fibrosis and its mechanisms

Antioxidant and Antibacterial Activities of the Tropical Red Alga Eucheuma spinosum: In Silico Study

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The Effects ofEucheuma cottoniion Signaling Pathway Inducing Mucin Synthesis in Rat Lungs Chronically Exposed to Particulate Matter 10 (PM₁₀) Coal Dust