The Effects of Some Electrolytes on the Buffering Capacity of Bacterium Coli

Shaughnessy, Howard J.; Falk, I. S.

doi:10.1128/jb.9.6.559-579.1924

Cited by 9 publications

(8 citation statements)

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“…1. The earlier work of Shaughnessy and Falk (1924) has shown that cells of Bact. coli in the zone of physiological interest possess appreciable capacity to resist changes in reaction in such a manner as to avoid injury to the cell.…”

Section: Discussionmentioning

confidence: 97%

“…In a previous communication from this laboratory (Shaughnessy and Falk, 1924) it was shown that the cells of Bact. coli exert a marked buffering effect when suspended in distilled water, an effect which rises to a maximum in the range favorable to viability (pH 6.0 to 6.9) and which gradually falls off with increasing acidity or alkalinity.…”

Section: Introductionmentioning

confidence: 95%

“…The HCl solutions used for titration and for adjusting the pH of the solutions was standardized by the potentiometric method, using the apparatus described by Shaughnessy and Falk (1924). The NaOH solution used for the titrations was standardized against the HCl solution daily.…”

Section: General Technique Employed In Our Experimentsmentioning

confidence: 99%

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The Diffusion Products of Bacterial Cells as Influenced by the Presence of Various Electrolytes

Shaughnessy¹,

Winslow²

1927

J Bacteriol

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 95%

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The Diffusion Products of Bacterial Cells as Influenced by the Presence of Various Electrolytes

Shaughnessy¹,

Winslow²

1927

J Bacteriol

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“…The concentrations of hydrogen ions were not the same in suspensions containing different neutral salts because different salts affect the buffering action of a bacterial suspension in different ways (Shaughnessy and Falk, 1924). Divalent cations prevented the production of alkaline substances more than monovalent cations, giving more acid suspensions.…”

Section: Data Obtained In Buffered Solutionsmentioning

confidence: 99%

The Endogenous Respiration of Bacillus cereus

Ingram

1939

J Bacteriol

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This paper deals with the action of salts on respiration in the absence of substrate-the endogenous respiration of Stier and Stannard (1935). The use of substrates which permit cell division has been avoided because salts influence cell multiplication, creating difficulties in interpreting the results in terms of the effect of the salts upon respiration. Moreover, measurements of gaseous exchange in the presence of substrate may give a misleading picture of the respiratory activity of a bacterial suspension owing to the existence of concurrent anabolic reactions, even where the number of cells does not change, as in the case of washed cells (Giesberger, 1936;and Barker, 1936). The effects of the components of the buffer mixture were first assessed, since they were expected to be similar to those of other salts. Thus, the investigation was begun by studying the effect of salts in unbuffered solutions without substrate, and the data so obtained were applied to the interpretation of experiments in which the effect of salts was studied in buffered solutions. II. EXPERIMENTAL PROCEDUREThe comparison of the effects caused by a variety of salts necessitated the preparation of a series of cultures of the same physiological age, washed so as to remove external nutrients.Suspensions of these cells were mixed with salt solutions, and the consumption of oxygen was then followed in manometers. 613 on July 8, 2020 by guest

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“…We are not aware of any preceding researches on the viscosities of bacterial suspension. The experments reported here were undertaken to determine the influences of H-ion concentrations upon the viscosities of such suspensions, working with a strain of Bacterium coli which has been utilized in extensive studies on the effects of ions upon viability , upon the electrophoretic potentials of bacteria (Winslow, Falk and Caulfield, 1923;Winslow and Shaughnessy 1924) and upon the buffering properties of bacteria (Shaughnessy and Falk, 1924).…”

Section: Introduction and Theorymentioning

confidence: 99%