The endangered species Brycon orbignyanus: genetic analysis and definition of priority areas for conservation

Ashikaga, Fernando Yuldi; Órsi, Mário Luís; Oliveira, Cláudio; Senhorini, José Augusto; Foresti, Fausto

doi:10.1007/s10641-015-0402-8

Cited by 32 publications

(40 citation statements)

References 54 publications

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“…Therefore, it is suitable for captive breeding (Borba et al 2006) and sport fishing from fish farms. However, anthropic factors, including overfishing, pollution and habitat fragmentation for hydroelectricity generation have caused a significant decrease in the natural populations of this species (Ashikaga et al 2015;Lima 2017), making it critically endangered in specific locations (Lima 2017).…”

Section: Introductionmentioning

confidence: 99%

“…However, previous data on the genome of the species being studied are necessary, and the use of microsatellites is restricted when species-specific primers are not available for genetic studies. Recently, the effort to use heterologous loci in B. orbignyanus has hampered the work of researchers, limiting the number of loci used (Lopera-Barrero et al 2014;Ashikaga et al 2015), due to the occurrence of null alleles (Henriques et al 2017). Thus, efforts that enable the design and use of molecular markers to evaluate genetic variability in populations and guide conservation programmes are valuable.…”

Section: Introductionmentioning

confidence: 99%

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Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Souza

Urrea-Rojas

Ruas

et al. 2018

Italian Journal of Animal Science

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Brycon orbignyanus is a neotropical fish found in several South American countries. This species has high ecological importance and is included in the list of fish threatened with extinction. In this study, we report the development and characterisation of 12 microsatellite primers of B. orbignyanus for future genetic studies. Screening of the 12 markers in a sample of 35 individuals identified seven polymorphic loci and 22 alleles, with one to three alleles per locus. The polymorphic information content ranged from 0.054 to 0.542, with four loci showing significant deviation from Hardy-Weinberg equilibrium. Two loci possessed null alleles. Cross-species amplification tests in two closely related species showed that 11 loci are transferrable to Brycon gouldingi (B. gouldingi) and nine are transferrable to B. falcatus. The microsatellite primers designed may be used for genetic variability and population structure studies in wild populations, fish farms and restocking programmes of B. orbignyanus and the two related species. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Souza

Urrea-Rojas

Ruas

et al. 2018

Italian Journal of Animal Science

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“…It is important to note that if a Bonferroni correction was applied, given the number of multiple tests performed, i.e., 75, even under the new significance value of ~0.0006, only BoM6 would pass the criteria for HWE. Lopera-Barrero et al Recently, Ashikaga et al (2015) reported BoM6 polymorphic amplifications in different stocks from the Alto Paraná river basin, detecting null alleles at the BoM6 and BoM13 loci. According to Lopera-Barrero (2010), BoM5 is monomorphic, although Rodriguez-Rodriguez et al (2010) observed otherwise.…”

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confidence: 99%

“…Later, four loci from B. opalinus (BoM1, BoM2, BoM7, and BoM13) were used in B. orbignyanus by Rodriguez-Rodriguez et al (2010) and five (BoM1, BoM2, BoM5, BoM7, and BoM13) by Lopera-Barrero et al (2010) in breeding stocks for population enhancement programs. More recently, Ashikaga et al (2015) evaluated the genetic structure of free-living populations of B. orbignyanus using four heterologous loci (BoM6 and BoM13, along with Bh5 and Bh13).…”

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Optimization of heterologous microsatellites in Piracanjuba

Carmo

Polo

Silva

et al. 2015

Pesq. agropec. bras.

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The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba.Index terms: Brycon orbignyanus, aquaculture, hatchery, molecular markers. Otimização de microssatélites heterólogos em piracanjubaResumo -O objetivo deste trabalho foi avaliar 41 marcadores microssatélites para amplificação heteróloga em piracanjuba (Brycon orbignyanus). Alguns marcadores foram testados pela primeira vez. Os loci foram otimizados para condições de PCR e aplicados a uma amostra de 49 indivíduos. Trinta e um loci resultaram em formação de produtos de PCR, enquanto dez loci resultaram em padrões polimórficos inteligíveis na amostra avaliada e podem ser usados para amplificações nessa espécie. Dos marcadores avaliados, quatro loci (BoM1, BoM13, Bh6 e Bh16) são válidos para aplicação em estudos em piracanjuba.Termos para indexação: Brycon orbignyanus, aquacultura, procriação, marcadores moleculares.

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Enhancing capacity for freshwater conservation at the genetic level: a demonstration using three stream macroinvertebrates

Macdonald

Ormerod

Bruford

2016

Aquatic Conservation

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1. Species diversity is declining more rapidly in freshwater ecosystems than in any other, but the consequences for genetic diversity, and hence evolutionary potential, are poorly understood. In part this reflects limited use and development of modern molecular tools and genetic approaches to address conservation questions in rivers, lakes and wetlands. As widespread, diverse and functionally important organisms, freshwater macroinvertebrates are ideal candidates for genetic approaches to reveal, for example, the conservation consequences of demographic histories and past disturbances. However, the availability of microsatellite markers for this group is very limited.2. Using next generation sequencing, microsatellite markers were developed for Isoperla grammatica (Poda, 1761), Amphinemura sulcicollis (Stephens, 1836) and Baetis rhodani (Pictet, 1843) to enable conservation genetic investigations of these widespread invertebrate species. Fifty-two robust microsatellite loci were developed (18, 21 and 13 per species), all with high levels of allelic diversity (7-27, 3-16, 5-13 alleles per loci, respectively).3. These tools will allow assessment of genetic structure, dispersal and demographic resilience in these model species as a function of environmental change and variation, thereby aiding freshwater monitoring and conservation. The authors urge further capacity building to support genetic applications to the conservation biology of other aquatic organisms.

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The endangered species Brycon orbignyanus: genetic analysis and definition of priority areas for conservation

Cited by 32 publications

References 54 publications

Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Novel microsatellite markers for the endangered neotropical fish Brycon orbignyanus and cross-amplification in related species

Optimization of heterologous microsatellites in Piracanjuba

Enhancing capacity for freshwater conservation at the genetic level: a demonstration using three stream macroinvertebrates

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