The Extensive Regulation of MicroRNA in Immune Thrombocytopenia

Zhao, Yong; Cui, Shangbin; Wang, Yan; Xu, Ruirong

doi:10.1177/10760296221093595

Cited by 16 publications

(7 citation statements)

References 192 publications

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“…Thus, it can act as a modulator of the immune system and can play an important role in the development of several autoimmune diseases [ 15 ]. Several studies have suggested that miRNAs are involved in the pathogenesis of ITP, because they can interact with the function of adaptive and innate immune responses [ 16 ]. The relationship between the mechanism of action of miRNA and ITP is complex and still only partially known.…”

Section: Discussionmentioning

confidence: 99%

Predictor Factors for Chronicity in Immune Thrombocytopenic Purpura in Children

et al. 2023

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(1) Background: Immune thrombocytopenia (ITP) is an acute autoimmune blood disorder that is the main cause of thrombocytopenia in children. It is characterized by a decrease in platelets below 100 × 109/L, and limited evolution with severe complications such as intracranial hemorrhage. The chronic form is defined by the persistence of thrombocytopenia more than 12 months after diagnosis. (2) Methods: We performed a retrospective study over a period of 10 years (1 January 2011–31 December 2020) at the Emergency Clinical Hospital for Children “Sf. Maria”, Iasi. The aim of the study was to describe the clinical characteristics and to determine the prognostic factors in immune thrombocytopenia in children. (3) Results: In this study we included 271 children with ITP, comprising 123 females (45.4%) and 148 males (54.6%). The remission rate was higher in males, being 68.9% compared to 56.1% in females. Children with ITP under 9 years of age had a higher remission rate. Children with a platelet count > 10 × 109/L at diagnosis had a higher likelihood-of-remission rate compared to patients who presented initial platelet count below this value. (4) Conclusions: The risk factors highly suggestive for chronicity are: age at diagnosis, female sex, and the number of platelets at the onset of the disease.

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Section: Discussionmentioning

confidence: 99%

Predictor Factors for Chronicity in Immune Thrombocytopenic Purpura in Children

et al. 2023

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“…miR-4482 has been previously reported to have differential expression in several diseases such as pediatric embryonal central nervous system neoplasms [ 34 ], immune thrombocytopenia [ 35 , 36 ], and infections [ 37 ]. Similarly, miR-3912 is differentially expressed in papillary thyroid cancer patients [ 38 ] and pediatric embryonal central nervous system neoplasms [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

miR-4482 and miR-3912 aim for 3ʹUTR of ERG mRNA in prostate cancer

et al. 2023

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Ets-related gene (ERG) is overexpressed as a fusion protein in prostate cancer. During metastasis, the pathological role of ERG is associated with cell proliferation, invasion, and angiogenesis. Here, we hypothesized that miRNAs regulate ERG expression through its 3ʹUTR. Several bioinformatics tools were used to identify miRNAs and their binding sites on 3ʹUTR of ERG. The selected miRNAs expression was analyzed in prostate cancer samples by qPCR. The miRNAs overexpression was induced in prostate cancer cells (VCaP) to analyze ERG expression. Reporter gene assay was performed to evaluate the ERG activity in response to selected miRNAs. The expression of ERG downstream target genes was also investigated through qPCR after miRNAs overexpression. To observe the effects of selected miRNAs on cell proliferation and migration, scratch assay was performed to calculate the cell migration rate. miR-4482 and miR-3912 were selected from bioinformatics databases. miR-4482 and -3912 expression were decreased in prostate cancer samples, as compared to controls (p<0.05 and p<0.001), respectively. Overexpression of miR-4482 and miR-3912 significantly reduced ERG mRNA (p<0.001 and p<0.01), respectively) and protein (p<0.01) in prostate cancer cells. The transcriptional activity of ERG was significantly reduced (p<0.01) in response to miR-4482 and-3912. ERG angiogenic targets and cell migration rate was also reduced significantly (p<0.001) after miR-4482 and -3912 over-expression. This study indicates that miR-4482 and -3912 can suppress the ERG expression and its target genes, thereby, halt prostate cancer progression. These miRNAs may be employed as a potential therapeutic target for the miRNA-based therapy against prostate cancer.

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“…Veiga et al found that miR-155 is a key positive regulator of regulatory T cells (Tregs) [22] . At the same time, studies have shown that miRNAs play an important role in hematopoietic differentiation, which is related to the cellular immune response leading to ITP [23] . Zuo et al found that miR-4739 significantly increased in ITP [24] .…”

Section: And Figmentioning

confidence: 99%

Analysis of the Mechanism of T Lymphocytes Promoting Immune Platelet Transfusion Refractoriness by Gene Chip Technique

Song¹,

Luo²,

Wang³

et al. 2023

IJPS

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The main objective of this study is to analyze the expression levels of messenger ribonucleic acid and long non-coding ribonucleic acid in patients with platelet transfusion refractoriness and reveal the mechanism of T lymphocytes in immune platelet transfusion refractoriness. The Agilent expression profile chip was used to detect the expression levels; gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis were performed on the differential genes to determine their main biological functions. Unsupervised hierarchical clustering was used to process differential genes and the differential genes among samples were represented in a heat map. The differentially expressed messenger ribonucleic acids and long non-coding ribonucleic acids in different groups were found as 720 and 1719 in normal control group vs. platelet transfusion effective group; 4254 and 12491 in normal control group vs. platelet transfusion ineffective group and 1806 and 6216 in platelet transfusion effective group vs. platelet transfusion ineffective group. Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis were performed on differentially expressed genes, and the results were annotated to be related to T cells. The co-expression of the target gene Ras-related protein 1A and long non-coding transactive response deoxyribonucleic acid binding protein 2 was determined through the national center for biotechnology information database and the interaction between micro ribonucleic acid-4739 and Ras-related protein 1A was predicted using the starBase and TargetScan databases. T lymphocytes play an important role in immune platelet transfusion refractoriness and long non-coding transactive response deoxyribonucleic acid binding protein 2 may affect the differentiation of T lymphocytes and promote the occurrence of immune platelet transfusion refractoriness through micro ribonucleic acid-4739 targeting Rasrelated protein 1A gene regulation.

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The Extensive Regulation of MicroRNA in Immune Thrombocytopenia

Cited by 16 publications

References 192 publications

Predictor Factors for Chronicity in Immune Thrombocytopenic Purpura in Children

Predictor Factors for Chronicity in Immune Thrombocytopenic Purpura in Children

miR-4482 and miR-3912 aim for 3ʹUTR of ERG mRNA in prostate cancer

Analysis of the Mechanism of T Lymphocytes Promoting Immune Platelet Transfusion Refractoriness by Gene Chip Technique

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