The ganglioside GD1α, IV3Neu5Ac, III6Neu5Ac-GgOse4Cer, is a major disialoganglioside in the highly metastatic murine lymphoreticular tumour cell line MDAY-D2

Müthing, Johannes; Peter‐Katalinić, Jasna; Hanisch, Franz‐Georg; Unland, Frank; Lehmann, Jürgen

doi:10.1007/bf00731155

Cited by 21 publications

(9 citation statements)

References 52 publications

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“…For instance, the ganglioside GD series (i.e. GD1, GD2, and GD3) may contain nervonic acid in their structures [41–43]. Previously, it was reported GD3 is associated with type 2 diabetes [44].…”

Section: Discussionmentioning

confidence: 99%

Plasma phospholipids, non-esterified plasma polyunsaturated fatty acids and oxylipids are associated with BMI

Pickens

Sordillo

Comstock

et al. 2015

Prostaglandins, Leukotrienes and Essential Fatty Acids

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The obese lipid profile is associated with increased free fatty acids and triacylglycerides. Currently, little is known about the plasma lipid species associated with obesity. In this study, we compared plasma lipid fatty acid (FA) profiles as a function of BMI. Profiling phospholipid (PL) FAs and their respective oxylipids could predict which obese individuals are more likely to suffer from diseases associated with chronic inflammation or oxidative stress. We investigated the relationship between BMI and plasma PL (PPL) FA composition in 126 men using a quantitative gas chromatography analysis. BMI was inversely associated with both PPL nervonic and linoleic acid (LA) but was positively associated with both dihomo-γ-linolenic and palmitoleic acid. Compared to lean individuals, obese participants were more likely to have ω-6 FAs, except arachidonic acid and LA, incorporated into PPLs. Obese participants were less likely to have EPA and DHA incorporated into PPLs compared to lean participants. Non-esterified plasma PUFA and oxylipid analysis showed ω-6 oxylipids were more abundant in the obese plasma pool. These ω-6 oxylipids are associated with increased angiogenesis (i.e. epoxyeicosatrienoates), reactive oxygen species (i.e. 9-hydroxyeicosatetraenoate), and inflammation resolution (i.e. Lipoxin A4). In summary, BMI is directly associated with specific PPL FA and increased ω-6 oxylipids.

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Section: Discussionmentioning

confidence: 99%

Plasma phospholipids, non-esterified plasma polyunsaturated fatty acids and oxylipids are associated with BMI

Pickens

Sordillo

Comstock

et al. 2015

Prostaglandins, Leukotrienes and Essential Fatty Acids

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show abstract

“…Besides, many antibodies are now known to cross‐react with two or more gangliosides and/or even glycoprotein species carrying the same carbohydrate epitope, which in some cases may lead to misidentification. The best results concerning unequivocal and detailed structural characterization of ganglioside samples available in small amounts are obtained by combining mass spectrometric and the immunochemical methods 24–27. In comparison to LSIMS high‐energy collision‐induced dissociation (CID) MS/MS,28 electrospray ionization (ESI) combined with low‐energy CID MS/MS provides higher sensitivity and fragmentation efficiency of ganglioside ions.…”

Section: Introductionmentioning

confidence: 99%

Nano-electrospray ionization time-of-flight mass spectrometry of gangliosides from human brain tissue

2001

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A general approach for the detection and structural elucidation of brain ganglioside species GM1, GD1 and GT1 by nano-electrospray ionization quadrupole time-of-flight (nanoESI-QTOF) mass spectrometry (MS), using combined data from MS and MS/MS analysis of isolated native ganglioside fractions in negative ion mode and their permethylated counterparts in the positive ion mode is presented. This approach was designed to detect and sequence gangliosides present in preparatively isolated ganglioside fractions from pathological brain samples available in only very limited amounts. In these fractions mixtures of homologue and isobaric structures are present, depending on the ceramide composition and the position of the sialic acid attachment site. The interpretation of data for the entire sequence, derived from A, B, C and Y ions by nanoESI-QTOFMS/MS in the negative ion mode of native fractions, can be compromised by ions arising from double and triple internal cleavages. To distinguish between isobaric carbohydrate structures in gangliosides, such as monosialogangliosides GM1a and GM1b, disialogangliosides GD1a, GD1b and GD1c or trisialogangliosides GT1b, GT1c and GT1d, the samples were analysed after permethylation in the positive ion nanoESI-QTOFMS/MS mode, providing set of data, which allows a clear distinction for assignment of outer and inner fragment ions according to their m/z values. The fragmentation patterns from native gangliosides obtained by low-energy collision induced dissociation (CID) by nanoESI-QTOF show common behaviour and follow inherent rules. The combined set of data from the negative and positive ion mode low-energy CID can serve for the detection of structural isomers in mixtures, and to trace new, not previously detected, components.

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“…According to Spearman (Olds, 1938(Olds, , 1949, the formula for the rank correlation coefficient r, is given by 6 C d f rs=l--n 3 -n r, has a range from -1 to + 1 and the values are tabulated (Siege1 and Castellan, 1988). This non-parametric test reveals whether experimental values obtained by different procedures are significantly alike (Muthing and Unland, 1994). Rank correlation coefficient (r,) values sO.83 indicate no significant correlation on the 5% significance level (p=0.95 and n=6).…”

Section: Methodsmentioning

confidence: 99%

Replacement of Chloroform Throughout Glycosphingolipid Isolation

Heitmann

Lissel

Kempken

et al. 1996

Biomed. Chromatogr.

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The ganglioside GD1α, IV3Neu5Ac, III6Neu5Ac-GgOse4Cer, is a major disialoganglioside in the highly metastatic murine lymphoreticular tumour cell line MDAY-D2

Cited by 21 publications

References 52 publications

Plasma phospholipids, non-esterified plasma polyunsaturated fatty acids and oxylipids are associated with BMI

Plasma phospholipids, non-esterified plasma polyunsaturated fatty acids and oxylipids are associated with BMI

Nano-electrospray ionization time-of-flight mass spectrometry of gangliosides from human brain tissue

Replacement of Chloroform Throughout Glycosphingolipid Isolation

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