The Hypo-Osmotic Swelling Test in Fresh Garut Ram Spermatozoa

Abstract

ABSTRAKPenelitian ini bertujuan untuk menentukan waktu optimum untuk pengujian membran plasma yang utuh (MPU) pada sperma domba garut setelah inkubasi pada larutan hypo-osmotic. Sebanyak 24 ejakulat dari 8 ekor semen domba garut yang telah dewasa kelamin dikoleksi menggunakan vagina buatan. Semen dievaluasi secara makroskopis selanjutnya dilakukan pengujian MPU dengan mengambil 10 µL semen segar dan dimasukkan ke dalam 2 mL larutan hypo-osmotic kemudian diinkubasi pada suhu 37 o C selama 1 jam. Sample dievaluasi setiap 15 menit dengan menghitung 200 sperma yang bereaksi (coil) dan yang tidak bereaksi dari lima lapang pandang. Hasil penelitian menunjukkan waktu optimal pengujian MPU menggunakan larutan hypo-osmotic adalah 30 menit dan tidak ada perbedaan antar 8 domba jantan yang digunakan Kata Kunci : Keutuhan membran plasma, Semen domba garut, HOS test ABSTRACTThe study was aimed to determine the optimal time to evaluate the maximum numbers of swollen sperm after expose to hypo-osmotic solution for testing membrane integrity in fresh garut ram semen. A total of 24 ejaculate samples from eight sexually mature garut ram were collected using artificial vagina. After macroscopic evaluation, 10 µL undiluted semen was gently mixed in each of the 2 mL hypoosmotic solution and incubated at 37 o C for 1 hour. Two hundred of coil and non-coiled sperm were observed from five different fields every 15 minutes. Results demonstrated that the maximum percentage of coiling sperm positive to HOS test was at 30 minutes and no differences among 8 garut rams used.

Qualitative ultrastructural changes and morphometry of deccani sheep spermatozoa preserved with egg yolk citrate extender

“…The semen colour and consistency of Deccani ram was creamy white and thick in the present study which was similar to that of Ossimi, Barki, Rahmani, Awassi, Finnish ram semen (creamy and thick) (Kayali et al, 2014). The average semen consistency of Deccani ram was thick in the present study while moderate consistency was reported for Garut ram semen (Nalley et al, 2013). The variation may be due to sperm concentration (Youngquist, 2007) and in the present study the breed produced average semen concentration of 10386 ± 537 millions/ml which was much higher compared to the concentration of Garut rams (Boediono et al, 2004).…”

Impact of Conventional and Non-Conventional Extenders on Rams Semen Quality During Storage at 5ºC

“…On the other hand, the worst values was observed using E4 extender. The hypo-osmotic swelling test (HOST) has proved to be a good tool for evaluating the membrane integrity of sperm of various domestic animals (Nalley and Arifiantini, 2013). The current results recorded that primary ram spermatozoa characteristic of POR (%) at zero time was nonsignificant (92.38, 93.38, 91.12, 90.62 and 93.25%) with E1, E2, E3, E4 and E5 extenders, respectively.…”

Correlation Between Hypo-Osmotic Swelling Test (Host) and Other Seminal Characteristics of Deccani Ram Semen

“…HOST is used to characterize the plasma membrane integrity of ram semen by means of "curling" pattern of sperm tail, which occurs due to influx of water when maintained under hypotonic environment. Nalley & Arifiantini (2013) evaluated plasma membrane integrity of Indonesian Garut ram semen (fresh) and reported 69.47% of swollen sperm when subjected to HOST. In the present study, the mean percentage of the sperm positive to HOST was lower compared to that of Indigenous ram semen of Bangladesh (69%) diluted with Tris based extender (Azizunnesa et al, 2014).…”