The purpose of this study is to measure the quality perception of in-flight services for the passengers. For that purpose, the service quality performances of the two biggest Turkish low-cost airlines were evaluated within the scope of the study. The study was designed quantitatively, and the survey technique was used to collect data. The data was obtained with convenient sampling method by involving 608 participants using the package program. In the analysis, factor, frequency and reliability analysis, t-test, and oneway ANOVA tests were used. According to the results of the study, both of the Turkish low-cost airlines have high service quality scores. Another conclusion of the study is that the demographic differences are not a determinant in airline service quality perception.