The In Vitro Ability of Klebsiella pneumoniae to Form Biofilm and the Potential of Various Compounds to Eradicate It from Urinary Catheters

Abstract

Urinary infections related to the presence of bacterial biofilm on catheters are responsible for loss of patients’ health and, due to their high frequency of occurrence, generate a significant economic burden for hospitals. Klebsiella pneumoniae is a pathogen frequently isolated from this type of infection. In this study, using a cohesive set of techniques performed under stationary and flow conditions, we assessed the ability of 120 K. pneumoniae strains to form biofilm on various surfaces, including catheters, and evaluated the usefulness of clinically applied and experimental compounds to remove biofilm. The results of our study indicate the high impact of intraspecies variability with respect to K. pneumoniae biofilm formation and its susceptibility to antimicrobials and revealed the crucial role of mechanical flushing out of the biofilm from the catheter’s surface with use of locally active antimicrobials. Therefore, our work, although of in vitro character, may be considered an important step in the direction of efficient reduction of K. pneumoniae biofilm-related hospital infections associated with the presence of urine catheters.

Analysis of Capped Silver Nanoparticles Combined with Imipenem against Different Susceptibility Profiles of Klebsiella pneumoniae

“…Moderate adhesion can be interpreted as positive biofilm production according to the analysis proposed by Stepanovic et al [ 50 ]. The quantification of biofilm biomass is presented in the study of Oleksy-Wawrzyniak and co-authors [ 51 ], where they analyzed the adherence of 118 isolates of K. pneumoniae on polystyrene plates from the crystal violet assay, and 51% of isolates showed biofilms with moderate adherence. This study also demonstrated K. pneumoniae ATCC 700603 adherence with the obtained value of OD 1.00 unit ± 0.35, corroborating the results shown in Figure 4 b.…”

Biofilm-forming microorganisms causing hospital-acquired infections from intravenous catheter: A systematic review

“…Regarding the results obtained in the study set, more than 87% of Gram-negative isolates are biofilm producers, in agreement with the literature, being detected around 84% in CVC-related infections with E. coli, K. pneumoniae, P. aeruginosa, and Acinetobacter spp. Consequently, urge for new therapies are urgently needed to inhibit and eradicated biofilms in catheter-associated infections, as postulated by several authors ( Gunardi et al., 2021 ; Maharjan et al., 2018 ; Oleksy-Wawrzyniak et al., 2021 ). Finally, Candida isolates equally demonstrated to be strong biofilm-formers, through their production of extracellular polysaccharides and hyphae morphology shift, showing a biofilm prevalence near 100% and so becoming an important threat in hospital-acquired infections ( Bekkal brikci benhabib et al., 2021 ).…”

Enhancement of Bacterial Anti-Adhesion Properties on Robust PDMS Micro-Structure Using a Simple Flame Treatment Method

“…by shaking at 150 rpm for 30 min. The absorption of the staining cell solution was measured at the wavelength of 591 nm to determine the bacterial adhesion on the sample surfaces [ 42 ]. Thus, the absorbance ratio of crystal violet stain and initial cell density was determined at the wavelengths of 591 and 570 nm (A591 nm/A570 nm).…”