The in vitro and in vivo anti-hepatotoxic, anti-hepatitis B virus and hepatic CYP450 modulating potential of Cyperus rotundus

Parvez, Mohammad K.; Al‐Dosari, Mohammed S.; Arbab, Ahmed H.; Niyazi, Sakina

doi:10.1016/j.jsps.2019.02.003

Cited by 30 publications

(14 citation statements)

References 39 publications

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“…In the present study, the decreases in plasma AST and ALP levels after PR intervention provided further evidence for the anti-inflammatory effect of PR. Additionally, AST, ALT, AST/ALT ratio, and ALP in plasma are good indicators of liver function 35 – 37 . Furthermore, one study found that PEDV-induced up-regulated proteins were involved in 22 diseases and disorders, such as hepatic diseases, gastrointestinal diseases, and inflammatory responses 9 .…”

Section: Discussionmentioning

confidence: 99%

Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus

Zhang

et al. 2021

Sci Rep

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Puerarin has been reported to be an excellent antioxidant, anti-inflammatory and antimicrobial agent, but the potential effect of puerarin on porcine epidemic diarrhea virus (PEDV) is unclear. This study aimed to determine whether puerarin could alleviate intestinal injury in piglets infected with PEDV. A PEDV (Yunnan province strain) infection model was applied to 7-day-old piglets at 104.5 TCID50 (50% tissue culture infectious dose). Piglets were orally administered with puerarin at the dosage of 0.5 mg/kg body weight from day 5 to day 9. On day 9 of the trial, piglets were inoculated orally with PEDV. Three days later, jugular vein blood and intestinal samples were collected. Results showed puerarin reduced morbidity of piglets infected with PEDV. In addition, puerarin reduced the activities of aspartate aminotransferase and alkaline phosphatase, the ratio of serum aspartate aminotransferase to serum alanine aminotransferase, the number of white blood cells and neutrophils, and the plasma concentrations of interleukin-6, interleukin-8 and tumor necrosis factor-α, as well as protein abundances of heat shock protein-70 in PEDV-infected piglets. Moreover, puerarin increased D-xylose concentration but decreased intestinal fatty acid-binding protein concentration and diamine oxidase activity in the plasma of piglets infected with PEDV. Puerarin increased the activities of total superoxide dismutase, glutathione peroxidase and catalase, while decreasing the activities of myeloperoxidase and concentration of hydrogen peroxide in both the intestine and plasma of PEDV-infected piglets. Puerarin decreased mRNA levels of glutathione S-transferase omega 2 but increased the levels of nuclear factor erythroid 2-related factor 2. Furthermore, puerarin increased the abundance of total eubacteria (16S rRNA), Enterococcus genus, Lactobacillus genus and Enterobacteriaceae family in the intestine, but reduced the abundance of Clostridium coccoides in the caecum. These data indicate puerarin improved intestinal function in piglets infected by PEDV and may be a promising supplement for the prevention of PEDV infection.

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Section: Discussionmentioning

confidence: 99%

Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus

Zhang

et al. 2021

Sci Rep

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“…The serum levels of the hepatic enzymes, ALT and AST, were measured to indicate liver cell damage ( 28 ), and the serum level of TBA was detected as an index of character cholestasis ( 29 ). Lipid profiles of TG, TC and LDL accumulations are involved in the pathogenesis of hepatotoxicity ( 30 ). Therefore, the present study measured these hepatotoxicity-related parameters, and it was found that mice in the STZ-HFD group showed significant liver injury similar to the findings of a previous study, in which the levels of AST, ALT and ALP were found increased in rats with STZ-induced diabetes ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

Role of Asxl2 in non‑alcoholic steatohepatitis‑related hepatocellular carcinoma developed from diabetes

Zhang

Teng

et al. 2020

Int J Mol Med

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The present study investigated the mechanism(s) of non-alcoholic steatohepatitis-related hepatocellular carcinoma (NASH-HCC) developed from diabetes. Streptozotocin and a high-fat diet (STZ-HFD) were used to induce NASH-HCC in ApoE −/− mice. Mouse liver functions were evaluated by H&E staining, liver/body weight and serum biochemical analysis. The expression levels of inflammation-associated factors were determined by RT-qPCR. Gene expression profiles related to molecular functions and pathways of NASH-HCC were examined by principal component analysis, heatmap, gene ontology and KEGG pathway enrichment analysis. Differentially expressed genes (DEGs) in tumor tissues were confirmed by RT-qPCR. The expression of Asxl2 in human NASH-HCC, other HCC tissues and HCC cells was measured by western blot (WB analysis) and RT-qPCR. For SNU-182 cells transfected with siAsxl2 or Hep3B cells with Asxl2 overexpression, cell proliferation, cell cycle, migration and invasion were respectively determined by CCK-8 assays, flow cytometry, wounding healing and Transwell assays. The expression levels of cell metastasis- and cycle-related proteins were determined by WB analysis and RT-qPCR. NASH-HCC model mice exhibited tumor protrusion with severe steatosis. The blood glucose concentration, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and low-density lipoprotein (LDL), total bile acid (TBA) and the levels of interleukin (IL)-6, tumor necrosis factor (TNF)-α, glypican 3 (GPC3) and transforming growth factor (TGF)-β were all increased in NASH-HCC model mice. DEGs were mainly related to chromosome organization, the cell cycle and the mitogen-activated kinase (MAPK) pathway. Asxl2 was significantly downregulated in HCC tissues and cells, and this regulated cell growth, migration and invasion. The gene expression pattern, related molecular functions and signaling pathways of NASH-HCC differed from those of normal liver tissues. Additionally, the downregulation of Asxl2 may play a potential role in development of NASH-HCC in patients with diabetes.

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“…Chikungunya virus 52 Andrographolide Vero cells in vitro anti-dengu viral activity 53 Cissampelos pariera Linn AG129 mouse model pan-DENV inhibitory activity 54 Cyperus rotundus Cyperene HepG2 cells Hepatitis B virus (HBV) 55 Several standardized protocols are available on authentication of herbs, isolation, characterization and estimation of active antiviral phytochemicals. Generally, in herbs active phytoconstituents exist along with other complex mixtures of compounds.…”

Section: Andrographolidementioning

confidence: 99%

A Review on Analytical Methods for Antiviral Phytoconstituents

Sivagami¹,

Sailaja²

2021

JYP

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Viral infections are spreading to a great extent worldwide and affecting many people. The existing and emerging viral infections are a threat to human beings. Combating against these viral infections is a challenge with the available few antiviral drugs. Though new Antiviral drugs are being approved by the FDA every year, controlling emerging viral infections is a global concern. The currently available drugs and vaccines for the treatment of existing viral infections have limitations. From ancient times, herbs played a significant role in treating viral disorders by developing immunity towards viral infections. Some more herbs with their antipyretic and analgesic activity helped in treating fever and body aches resulted of viral infections. Antiviral phytoconstituents like alkaloids, glycosides, flavonoids, terpenoids, polyphenols, coumarins and saponins of plants have been isolated from plants and studied for their antiviral effects by researchers. Some of the phytochemicals are even developed as formulations and marketed to treat viral infections. Quality control of phytochemicals and herbal formulations is necessary for assuring therapeutic efficacy, quality and safety of these preparations. Hence, various standardization methods are developed for ensuring the quality of herbal products. The current review encompasses the RP-HPLC, LC-MS, GC-MS and HPTLC methods available for the Single and concurrent estimation of different antiviral phytoconstituents.

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The in vitro and in vivo anti-hepatotoxic, anti-hepatitis B virus and hepatic CYP450 modulating potential of Cyperus rotundus

Cited by 30 publications

References 39 publications

Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus

Puerarin enhances intestinal function in piglets infected with porcine epidemic diarrhea virus

Role of Asxl2 in non‑alcoholic steatohepatitis‑related hepatocellular carcinoma developed from diabetes

A Review on Analytical Methods for Antiviral Phytoconstituents

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