The in vivo effect of P‐15 coating on early osseointegration

Abstract

The aim of this study was to evaluate mechanically and morphologically the effect of a specific peptide sequence P-15, when incorporated into implant surfaces. Three types of implants were used for the study: Group A: commercially pure titanium implant (blasted and acid etched) + electrochemical thin calcium phosphate deposition, Group B: commercially pure titanium implant (blasted and acid etched) + electrochemical thin calcium phosphate deposition + P-15 incorporation, and as control, Group C: commercially pure titanium implant (blasted and acid etched). After a topographical characterization, transcortical osteotomies were made, and all implant groups (102 implants per group) were randomly placed bilaterally in the tibiae of adult beagle dogs (n = 24). At, 1, 2, and 4 weeks post-surgery, the animals were sacrificed and the samples were retrieved for removal torque tests, for nano indentation, and for histomorphometrical analysis. The results (mean ± 95% CI) showed that Group B (34.4 ± 8.7%) presented statistically higher bone-to-implant contact than the other groups (A = 23.9 ± 7.8%; C = 21.7 ± 8.3%) at 1 week, indicating an enhanced osteogenesis due to the peptide incorporation. The results suggested that the incorporation of P-15 to implant surfaces increased its bioactivity and the effects were notable especially in the early stages of the healing process.

Calcium Phosphate Nanocoatings and Nanocomposites, Part I: Recent Developments and Advancements in Tissue Engineering and Bioimaging

“…Compared with the control group (i.e., no peptide), both low and high concentrations of P-15 peptide recorded an increase in peri-implant bone density at 30 days. Later, these observations were in agreement with the investigation of Coelho et al [41] in which the effect of P-15 incorporated into the surface of an electrochemically deposited calcium phosphate coating was investigated using a canine model. A statistically higher bone-toimplant contact was recorded at 1 week and based on these findings, they suggested that the incorporation of P-15 increased the bioactivity of a calcium phosphatecoated implant and the effects were notable especially in the early stages of the healing process.…”

“…When competing with collagen for cell binding, it has been shown that the P-15 sequence to be more effective than the RGD-containing peptides [39][40][41]. To demonstrate faster osseointegration, Lutz et al [40] coated the surfaces of acid-etched and grit-blasted dental implants with calcium phosphate and then with P-15 peptides.…”

Osseointegration: Hierarchical designing encompassing the macrometer, micrometer, and nanometer length scales

“…titanium-based alloys), intimate contact between bone and device will render the system biomechanical stability and load-bearing capability [18][19][20][21]. Such observation has been supported by over ten thousand scientific reports that are based on a large number of different devices surgically placed in bone through a large variation in surgical instrumentation technique and sequence.…”

In vivo evaluation of biofunctionalized implant surfaces with a synthetic peptide (P‐15) and its impact on osseointegration. A preclinical animal study

“…; Coelho et al. ). In previous studies by our working group, P‐15 was applied in conjunction with the DENTSPLY Implants ANKYLOS ® plus implant system improving the implant surface and boost osseointegration.…”

“…, ; Coelho et al. ). One biomolecule that may improve cell adhesion on implant surfaces is the peptide P‐15.…”