The influence of intrinsic coagulation pathway on blood platelets activation by oxidized cellulose

Abstract

Oxidized cellulose is an effective hemostat that works naturally to aid in blood coagulation. The mechanism of its action is not very well understood. Little effect on blood coagulation, but a pronounce decrease in platelet count has been reported upon the addition of the oxidized cellulose to the whole blood. As a marker of platelet activation and aggregation we used serotonin release reaction and turbidity changes in time. We found that oxidized cellulose did not activate washed platelets reconstituted in plasma-free medium or plasma-free medium with fibrinogen; no reduction of platelet count was observed. Serotonin release in platelet-rich plasma incubated with oxidized cellulose started in the range from 5 to 10 min. Serotonin release from platelets reconstituted in plasma deficient in either coagulation factor V, VIII, IX, or XII was delayed. Blood platelets activation by oxidized cellulose requires calcium ions present in dispersion of oxidized cellulose. Factor XIII deficiency had no influence on blood platelets activation by oxidized cellulose. Our results clearly indicate the significance of intrinsic coagulation pathway activation on blood platelets activation by oxidized cellulose and so indirectly on the hemostyptic effect of oxidized cellulose.

New Hemostatic Powder (GelitaCel Ca Powder<sup>TM</sup>) and Its Application in Cranial Neurosurgery

“…The mode of action of oxidized (regenerated) cellulose is not fully elucidated but it is believed to be both physical (mechanical compression and blood absorption) and chemical (surface interactions with proteins and platelets, and activation of the intrinsic and extrinsic pathways) [10]- [12]. Oxidized cellulose has a low pH that is also thought to be responsible for its hemostatic and bacteriostatic effect [11] [12].…”

“…Oxidized cellulose has a low pH that is also thought to be responsible for its hemostatic and bacteriostatic effect [11] [12]. Cellulose has to be applied manually and slightly pressed against the brain surface.…”

TOPICALLY-USED GENTAMICIN ATTACHED TO NANOFIBRE MDOCTM COMPARED WITH GARAMYCIN SCHWAMM^® IN AN ACUTE WOUND INFECTION MODEL. AN EXPERIMENTAL STUDY.

“…This cellulose has good hemostatic effect and facilitates blood clot formation, proven prior to our study, and is produced as SEAL-ON TM (HemCon Medical Technologies, Inc., U.S.A.) which is determined to stop bleeding [22][23]. Because it is very difficult to create a model of a chronic infected wound in a healthy laboratory animal we used the one with acute deep incisional infection.…”

Oxycellulose: Significant characteristics in relation to its pharmaceutical and medical applications

“…However, only a little effect on coagulation itself was observed when blood was incubated with OC. 55,56 OC is believed to support hemostasis by decreasing the pH and acting as an acid. This leads to nonspecific aggregation of platelets and generating an artificial clot.…”