The macroglomerular complex of the antennal lobe in the tobacco budworm moth Heliothis virescens  : specified subdivision in four compartments according to information about biologically significant compounds

Berg, Bente Gunnveig; Almaas, Tor Jørgen; Bjaalie, Jan G.; Mustaparta, Hanna

doi:10.1007/s003590050290

Cited by 130 publications

(173 citation statements)

References 17 publications

Supporting

Mentioning

155

Contrasting

Order By: Relevance

“…In Lepidoptera, the number of macroglomerular structures is thought to be correlated with the number of behaviourally active components forming the pheromone blend in the respective species (Hansson et al, 1992;Ochieng' et al, 1995;Todd et al, 1995;Berg et al, 1998). In L. botrana males, we found one large glomerulus close to the entrance of the antennal nerve.…”

Section: Discussionmentioning

confidence: 69%

“…The macroglomerular complex (MGC), a structure of enlarged glomeruli present only in the male AL, processes specifically sex pheromone information (for reviews see Mustaparta, 1996;Anton and Homberg, 1999;Hansson and Christensen, 1999). Each ORN type responding to one pheromone component projects to one MGC glomerulus in several moth species (Hansson et al, 1992;Ochieng' et al, 1995;Todd et al, 1995;Berg et al, 1998). By contrast, the representation within glomeruli that process non-pheromone odours is less evident.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Three-dimensional antennal lobe atlas of male and female moths, Lobesia botrana (Lepidoptera: Tortricidae) and glomerular representation of plant volatiles in females

Masante-Roca

Gadenne

Anton

2005

Journal of Experimental Biology

View full text Add to dashboard Cite

Spatiotemporal odour coding is thought to be linked closely with the specific glomerular anatomy of the primary olfactory centre. In most insects the number of the glomeruli within the antennal lobe is limited to fewer than 100, allowing their individual identification. In the grapevine moth, Lobesia botrana, a map of the antennal lobe glomeruli was reconstructed three-dimensionally, by comparing three different brains in males and females. The map of the antennal lobe of females served then as a basis to identify glomeruli containing dendritic arborisations of 14 physiologically characterised projection neurons. Projection neurons responding to the same plant compound did not always arborise in the same glomerulus and some neurons arborising in the same glomerulus responded to different compounds. Different zones of target glomeruli were, however, identified when pooling all neurons responding to one of two different compounds respectively (α-farnesene and nonatriene). All identified glomeruli of specifically responding projection neurons were situated close to the anterior surface of the antennal lobe. One broadly responding projection neuron arborised in a more posteriorly situated glomerulus. A local interneuron responding to only one compound was arborising densely in a neighbouring glomerulus and had sparse branches in all other glomeruli. These results are discussed with respect to plant odour processing and structure-function relations in antennal lobe neurons. The 3D AL atlas will, in the future, also be used to obtain a better understanding of coding mechanisms of grapevine odours in this pest insect.

show abstract

Section: Discussionmentioning

confidence: 69%

mentioning

confidence: 99%

Three-dimensional antennal lobe atlas of male and female moths, Lobesia botrana (Lepidoptera: Tortricidae) and glomerular representation of plant volatiles in females

Masante-Roca

Gadenne

Anton

2005

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…In male moths, for example, the detection of the sex pheromone emitted by females is assured by the presence of specific sensillum types on the antennal surface. Afferents from ORNs located in these sensilla project into the AL where they form, upon entering, the first glomerular station responsible for pheromone information processing, the macroglomerular complex (MGC, Hansson et al 1995;Berg et al 1998). In Manduca sexta, three glomeruli have been found in females that are functionally distinct though possibly equivalent to the three MGC glomeruli in males (Rospars and Hildebrand 2000).…”

Section: Sexual Dimorphism In the Antennal Lobementioning

confidence: 99%

Morphological characterization of the antennal lobes in the Mediterranean fruit fly Ceratitis capitata

et al. 2015

View full text Add to dashboard Cite

The medfly Ceratitis capitata is one of the most important pests for horticulture worldwide. The knowledge about anatomy and function of the medfly olfactory system is still limited. The first brain structure to process olfactory information in insects is the antennal lobe (AL), which is composed of its functional and morphological units, the olfactory glomeruli. Here, we present a morphological three-dimensional reconstruction of AL glomeruli in adult brains. We used unilateral antennal backfills of olfactory receptor neurons (ORNs) with neural tracers, revealing the AL structure. We recorded confocal stacks acquired from whole-mount specimens, and analyzed them with the software AMIRA. The ALs in C. capitata are organized in glomeruli which are more tightly packed in the anterior part than the posterior one. Axons of ORNs bilaterally connect the ALs through a commissure between the two ALs. This commissure is formed by several distinct fascicles. Contralateral dye transfer suggests the presence of gap junctions connecting ORNs from both antennae. There was no statistical difference between the average volumes of female ALs (204,166 ± 12,554 μm(3)) and of male ALs (190,287 ± 11,823 μm(3)). In most specimens, we counted 53 glomeruli in each AL, seven of which were sexually dimorphic in size

show abstract

“…These glomeruli are dedicated to receiving information regarding female-produced sex pheromones (Bretschneider, 1924;Koontz and Schneider, 1987). In several moth species, the number of MGC glomeruli equals the number of behaviorally relevant pheromone components, with each ORN type projecting to one MGC glomerulus (Hansson et al, 1992;Ochieng et al, 1995;Todd et al, 1995;Berg et al, 1998). The ORNs project into the AL, where input is relayed onto PNs.…”

Section: Introductionmentioning

confidence: 99%

Reversed functional topology in the antennal lobe of the male European corn borer

Kárpáti

Dekker

Hansson

2008

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY The European corn borer Ostrinia nubilalis (Hübner) is a model of evolution of sexual communication in insects. Two pheromone strains produce and respond to opposite ratios of the two pheromone components, Z11 and E11-tetradecenylacetate. The Z-strain uses a ratio of 97:3 of Z11:E11 tetradecenylacetate, whereas the E-strain uses a ratio of 1:99. We studied how the difference in male preference correlates with differences in wiring of olfactory input and output neurons in the antennal lobe (AL). Activity-dependent anterograde staining, intracellular recording and immunocytochemistry were used to establish the structure and function of male olfactory receptor neurons (ORNs) and AL projection neurons (PNs). Physiologically characterized neurons were reconstructed using confocal microscopy of α-synapsin stained ALs. The ALs of males and females in both strains had approximately 64 glomeruli. In males the macroglomerular complex (MGC) was morphologically similar in the two strains and consisted of two major compartments, a large, medial compartment folded around a smaller,lateral one. Extensive physiological and morphological analysis revealed that in both strains the major pheromone component-specific ORNs and PNs arborize in the medial MGC glomerulus, whereas those sensitive to the minor pheromone component arborize in the lateral glomerulus. In other words, the two strains have an indistinguishable MGC morphology, but a reversed topology. Apparently,the single-gene-mediated shift that causes a radical change in behavior is located upstream of the antennal lobes, i.e. at the ORN level.

show abstract

The macroglomerular complex of the antennal lobe in the tobacco budworm moth Heliothis virescens : specified subdivision in four compartments according to information about biologically significant compounds

Cited by 130 publications

References 17 publications

Three-dimensional antennal lobe atlas of male and female moths, Lobesia botrana (Lepidoptera: Tortricidae) and glomerular representation of plant volatiles in females

Three-dimensional antennal lobe atlas of male and female moths, Lobesia botrana (Lepidoptera: Tortricidae) and glomerular representation of plant volatiles in females

Morphological characterization of the antennal lobes in the Mediterranean fruit fly Ceratitis capitata

Reversed functional topology in the antennal lobe of the male European corn borer

Contact Info

Product

Resources

About