The mitochondrial genome of the giant panda tick <i>Haemaphysalis flava</i> (Acari, Ixodidae) from Southwest China

Wang, Chengdong; Wang, Lidan; Liu, Yunjian; Deng, Lei; Wei, Ming; Wu, Kai; Huang, Shan; Guo, Li; Huang, Yan; Zhang, Hemin; Li, Desheng

doi:10.1080/23802359.2020.1731350

Cited by 4 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ava and Ha. longicornis species (99.11% and 100% identity, respectively) identi ed in this work is similar to that reported in previous studies [32][33][34][35]. However, the single species of Ha.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Molecular Survey and Genetic Characterization of Hard Ticks from Chongming Island of Shanghai, China, Based on Mitochondrial Genome Fragments

Fei

Zhao

Yin

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Ticks have been reported across China, but information on ticks from Chongming Eco-Island of Shanghai is lacking. Understanding the distribution and genetic evolution of ticks on the island is crucial for tick-borne infection control. Methods Ticks were collected from eight towns and four protected areas of Chongming Island during April 2021-March 2022 and were morphologically identified under microscopy. Molecular characterization of the ticks was performed by PCR amplification and sequencing of 12S rRNA and co1 mitochondrial genes. Homology analyses with published sequences obtained from GenBank were conducted for genetic relationships based on neighbor-joining (NJ) analysis of the 12S rRNA and co1 gene loci. To assess genetic evolution, intraspecific and interspecific genetic distances and barcoding gap were assessed based on NJ-K2P analysis of the 12S rRNA locus. Furthermore, genetic diversity and neutrality tests were performed on 12S rRNA sequences. Results A total of 1417/1933 ticks were successfully amplified by PCR of the 12S rRNA and co1 loci. Sequencing and homology analyses revealed four species of ticks, including Haemaphysalis flava (1376/1417 [97.11%], Ha. longicornis (37/1417 [2.61%]), Ha. doenitzi (3/1417 [0.21%]), and Ha. japonica (1/1417 [0.07%]). The intraspecies identity was 100% for Ha. longicornis, 99.11% for doenitzi, and 96.17%, while Ha. japonica had 83.48% intraspecies identity. Phylogenetic analyses showed species specificity, which clustered with homologous sequences. The average K2P distances between and within the species were 0.1351 (range 0.0723–0.1960) and 0.0145 (range 0-0.0946), respectively. The maximum K2P distance within species was in Ha. japonica (0.0946), while the maximum interspecific K2P distance was 0.1960 between Ha. hystricis and Ha. japonica. Genetic diversity indices and neutrality tests revealed that Fu and Li’s D and Tajima’s D values were negative for all species (mean values − 0.9360 and − 1.0310, respectively), suggesting evidence of recent population expansion. Conclusions This study is the first to report the genetic characterization of ticks from Chongming Eco-Island of Shanghai. We identified four species of Haemaphysalis ticks that were genetically similar to other congeners reported in China or worldwide. The knowledge gained from this study serves as a basis to support intervention deployment for effective control of tick-borne infections.

show abstract

Section: Discussionsupporting

confidence: 89%

“…Haemaphysalis ava has a broad geographic distribution in Asia [2]. It was mainly distributed in Hubei Province [32], Hunan Province, and Southwest China [33]. Previous studies reported that Rhipicephalus sanguineus and Ha.…”

Section: Discussionmentioning

confidence: 99%

Molecular Survey and Genetic Characterization of Hard Ticks from Chongming Island of Shanghai, China, Based on Mitochondrial Genome Fragments

Fei

Zhao

Yin

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The mitochondrial genome (mitogenome) is a reliable marker for cryptic species identification, phylogenetic studies, and population genetics analysis, and has been widely used in animals [ 23 , 24 , 25 , 26 , 27 , 28 ], including in ticks [ 7 , 21 , 29 ]. In recent years, the number of sequenced mitogenomes of Ixodidae species has increased significantly, but the mitogenome studies on parasitic ticks of giant pandas have only been performed for three Haemaphysalis species [ 30 , 31 , 32 ]. The mitogenomes of another ten Ixodidae species infesting giant pandas have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Liu

et al. 2022

Genes

View full text Add to dashboard Cite

Ticks rank second in the world as vectors of disease. Tick infestation is one of the factors threatening the health and survival of giant pandas. Here, we describe the mitogenomes of Ixodes acutitarsus and Ixodes ovatus parasitizing giant pandas, and perform comparative and phylogenetic genomic analyses on the newly sequenced and other available mitogenomes of hard ticks. All six newly determined mitogenomes contain a typical gene component and share an ancient Arthropoda gene arrangement pattern. Our study suggests that I. ovatus is a species complex with high genetic divergence, indicating that different clades of I. ovatus represent distinct species. Comparative mitogenomic analyses show that the average A + T content of Ixodidae mitogenomes is 78.08%, their GC-skews are strongly negative, while AT-skews fluctuate around 0. A large number of microsatellites are detected in Ixodidae mitogenomes, and the main microsatellite motifs are mononucleotide A and trinucleotide AAT. We summarize five gene arrangement types, and identify the trnY-COX1-trnS1-COX2-trnK-ATP8-ATP6-COX3-trnG fragment is the most conserved region, whereas the region near the control region is the rearrangement hotspot in Ixodidae mitogenomes. The phylogenetic trees based on 15 genes provide a very convincing relationship (Ixodes + (Robertsicus + ((Bothriocroton + Haemaphysalis) + (Amblyomma + (Dermacentor + (Rhipicentor + (Hyalomma + Rhipicephalus))))))) with very strong supports. Remarkably, Archaeocroton sphenodonti is embedded in the Haemaphysalis clade with strong supports, resulting in paraphyly of the Haemaphysalis genus, so in-depth morphological and molecular studies are essential to determine the taxonomic status of A. sphenodonti and its closely related species. Our results provide new insights into the molecular phylogeny and evolution of hard ticks, as well as basic data for population genetics assessment and efficient surveillance and control for the giant panda-infesting ticks.

show abstract

“…The gene arrangement was identical to soft ticks and non-Australasian Ixodes species (Chen et al 2020). Four of these PCGs (NAD4, NAD4L, NAD1 and NAD5) were located on the light strand (L-strand), all other remaining PCGs were anchored on the heavy strand (H-strand) (Wang et al 2020). We observed that the size of 22 transfer RNA genes ranging from 56 bp (tRNA-Ser) to 68 bp (tRNA-Lys), nine tRNA genes (tRNA-Gln, tRNA-Cys, tRNA-Tyr, tRNA-Phe, tRNA-His, tRNA-Pro, tRNA-Leu1, tRNA-Leu2, tRNA-Val) were encoded on the L-strand.…”

mentioning

confidence: 92%

The complete mitochondrial genome of Ixodes vespertilionis (Acari: Ixodidae)

Zuo

Jiang

et al. 2021

Mitochondrial DNA Part B

View full text Add to dashboard Cite

Ixodes vespertilionis is a tick parasitizing on the bodies of bats. In our study, the complete mitogenome of I. vespertilionis was determined by using Illumina sequencing technology. The mitogenome was 14,559 bp in size and was predicted to encode 37 genes including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and one control region. The gene order of the mitogenome is identical to Argasidae and non-Australasian Prostriata. The phylogenetic analysis by the Maximum-likelihood method reveals that I. vespertilionis is phylogenetically closest to Ixodes simplex. These data provide novel reference for further studies on the population genetics and phylogenetics of ticks.

show abstract

The mitochondrial genome of the giant panda tick Haemaphysalis flava (Acari, Ixodidae) from Southwest China

Cited by 4 publications

References 13 publications

Molecular Survey and Genetic Characterization of Hard Ticks from Chongming Island of Shanghai, China, Based on Mitochondrial Genome Fragments

Molecular Survey and Genetic Characterization of Hard Ticks from Chongming Island of Shanghai, China, Based on Mitochondrial Genome Fragments

Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

The complete mitochondrial genome of Ixodes vespertilionis (Acari: Ixodidae)

Contact Info

Product

Resources

About