The NADPH oxidase OsRbohA increases salt tolerance by modulating K+ homeostasis in rice

Increased soil salinization is among the main factors that limits safe rice production. Arbuscular mycorrhizal fungi (AMF) have been shown to alleviate the toxic effects of salt stress in plants. However, more studies on AMF combined with other functional microorganisms are needed to further improve salt tolerance in rice. Therefore, the compound inoculum Funneliformis mosseae (Fm) together with two functional microorganisms, Piriformospora indica (Pi) and Agrobacterium rhizogenes (Ar) was evaluated for their effect on the rice growth, photosynthetic gas exchange parameters, ion homeostasis, and the expression of salt tolerance-related genes under 0, 80, 120 and 160 mM salt stress conditions. The results showed that: (1) the rice seedling biomass of the AMF compound inoculant treatment group was significantly higher than that of the non-inoculation treatment group (P < 0.05); (2) under NaCl stress, inoculation with AMF compound inoculants can activate the rice antioxidant enzyme system and improve osmoregulation ability; (3) AMF compound inoculants can increase the concentration of K+ in the plant and inhibit the transfer of Na+ to rice leaves, maintaining a high K+/Na+; and (4) AMF compound inoculants could induce and regulate the overexpression of genes related to salt tolerance, photosynthesis and ion homeostasis in rice, and improve the tolerance of rice under salt stress. Our study showed that AMF compound inoculants could improve the adaptability of rice under NaCl stress and promote plant growth by regulating the photosynthetic gas exchange parameter, reactive oxygen species (ROS) scavenging ability, and ion homeostasis of plants. These results suggest that AMF compound inoculants may play an important role in improving rice productivity in salinized soil. Graphical Abstract

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“…Mycorrhizal plants regulate the ion homeostasis of plants. This is consistent with the results obtained by Wang et al ( 2022a , b , c ).…”

Section: Discussionsupporting

confidence: 94%

Effects of AMF Compound Inoculants on Growth, Ion Homeostasis, and Salt Tolerance-Related Gene Expression in Oryza sativa L. Under Salt Treatments

Zhang

Shi

Zheng

et al. 2023

Rice

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“…For instance, Rodríguez et al [ 52 ] investigated the relationship between NOX, O 2 •− , and the activities of SOD, peroxidase, and ascorbate in the elongation zone of maize leaf segments subjected to step-wise NaCl treatments. Furthermore, similar relationships among NOX activity, K + levels, and H 2 O 2 /NO signaling have been studied in rice by Wang et al [ 53 ] and Ijaz et al [ 54 ]. Additionally, the role of H 2 O 2 signaling has been examined in cucumber by Kabała et al [ 55 ], and alkalization-mediated signaling has been explored in Arabidopsis by Sun et al [ 56 ].…”

Section: Introductionmentioning

confidence: 67%

NADPH oxidase-mediated reactive oxygen species, antioxidant isozymes, and redox homeostasis regulate salt sensitivity in maize genotypes

Rohman,

Islam,

Habib

et al. 2024

Heliyon

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“…Our previous studies revealed that GmMPK6 was activated and increased ROS generation under salt-stress conditions [ 27 , 28 ]; however, the regulation mechanism of how GmMPK6 increases ROS generation has not yet been elucidated. As Rboh was known to generate ROS in response to salt stress in several plant species [ 16 , 17 , 18 , 19 , 20 , 21 ], we investigated the correlation between GmMPK6 activity and Rboh expression in soybean under salt stress. GmRbohI1 , a homolog of Arabidopsis RbohD , exhibited the highest increase in expression among 17 Rboh genes of soybean under salt-stress conditions ( Figure S2 ).…”

Section: Discussionmentioning

confidence: 99%

“…The NtbHLH123 transcription factor also enhanced the salt tolerance of Nicotiana tabacum through the induction of the NtRboh gene and ROS production [ 20 ]. Moreover, it was revealed that K + homeostasis modulated by OsRbohA-mediated H 2 O 2 accumulation is important for salt tolerance in rice [ 21 ]. However, knowledge of the regulation mechanisms of Rboh in salt stress response is still limited in plants.…”

Section: Introductionmentioning

confidence: 99%

GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean

Son

Kim

et al. 2023

Antioxidants

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Salt stress is a critical environmental stress that impairs plant growth and development, especially in crop productivity; therefore, understanding the salt response in plants is the basis for their development of salt tolerance. Under salinity, soybean mitogen-activated protein kinase 6 (GmMPK6) is activated and positively regulates reactive oxygen species (ROS) generation. However, it is not yet elucidated how GmMPK6 regulates ROS generation and its role in salt tolerance. Here, we show that GmMPK6, solely activated in NaCl treatment, and gene expression of GmRbohI1 was not only reduced by MPK inhibitor SB202190 in NaCl treatment, but also increased in a GMKK1-expressing protoplast. Furthermore, SB202190 and the NADPH-oxidase inhibitor, diphenyleneiodonium chloride, increased susceptibility to salt stress. The expression of GmRD19A was induced by NaCl treatment, but this expression was compromised by SB202190. Consequently, we revealed that GmMPK6 induces ROS generation through the transcriptional regulation of GmRbohI1 and increases salt tolerance in soybean.

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The NADPH oxidase OsRbohA increases salt tolerance by modulating K+ homeostasis in rice

Cited by 17 publications

References 69 publications

Effects of AMF Compound Inoculants on Growth, Ion Homeostasis, and Salt Tolerance-Related Gene Expression in Oryza sativa L. Under Salt Treatments

Effects of AMF Compound Inoculants on Growth, Ion Homeostasis, and Salt Tolerance-Related Gene Expression in Oryza sativa L. Under Salt Treatments

NADPH oxidase-mediated reactive oxygen species, antioxidant isozymes, and redox homeostasis regulate salt sensitivity in maize genotypes

GmMPK6 Positively Regulates Salt Tolerance through Induction of GmRbohI1 in Soybean

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