The Number of Bleaching Sessions Influences Pulp Tissue Damage in Rat Teeth

Cintra, Luciano Tavares Ângelo; Benetti, Francine; Facundo, Aguinaldo Cândido da Silva; Ferreira, Leonardo L. G.; Gomes‐Filho, João Eduardo; Ervolino, Edílson; Rahal, Vanessa; Briso, André Luiz Fraga

doi:10.1016/j.joen.2013.08.007

Cited by 82 publications

(157 citation statements)

References 26 publications

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“…Based on the data obtained in this investigation as well as in previous in vivo studies [6,[20][21][22], new strategies for in-office bleaching therapy should be focused on preventing pulp damage and improving pulp cell regenerative potential. It was recently demonstrated that reducing the contact time of a 35%-HP gel (5-15 min) with enamel led to decreases in about 60% the transenamel and transdentinal diffusion of HP [26].…”

Section: Discussionmentioning

confidence: 78%

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Histopathological Features of Dental Pulp Tissue from Bleached Mandibular Incisors

Roderjan¹,

Stanislawczuk²,

Hebling³

et al. 2014

JMSE-B

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Mandibular incisors scheduled for extraction were subjected or not to three 15-minute applications of a 35% hydrogen peroxide gel. Two days thereafter, bleaching effectiveness (according to a value-oriented shade guide) was evaluated, and histological analysis of pulp tissue was performed. Immediately and two days after bleaching, tooth sensitivity (TS) experience was recorded. Bleached teeth of both groups showed significant color improvement (Mann-Whitney; p < 0.05). However, these teeth presented significant pulp alterations when compared with non-bleached teeth (Mann-Whitney; p < 0.05), including areas of superficial necrosis associated with mild inflammatory reactions. These data were achieved by recordings of TS immediately after bleaching (Fisher's exact test; p<0.05), which was reduced with time. Instead of 'no significant difference' being observed between young and old teeth (Mann-Whitney; p<0.05), necrosis occurred in 60% of old bleached teeth in comparison with 100% of young teeth, associated with underlying tertiary dentin deposition. It was concluded that, regardless of the age of patients, the application of a 35%-HP bleaching gel to mandibular incisors causes pulp damage, which was related to initial TS; however, superficial necrosis was more prevalent in young teeth.

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Section: Discussionmentioning

confidence: 78%

“…It was also shown that rat molars subjected to the same bleaching protocol as used in the present study presented coronal pulp necrosis associated with intense inflammatory reaction in the radicular pulp tissue. The authors demonstrated that repetition of tooth-bleaching sessions resulted in greater pulp necrosis [22].…”

Section: Discussionmentioning

confidence: 99%

Histopathological Features of Dental Pulp Tissue from Bleached Mandibular Incisors

Roderjan¹,

Stanislawczuk²,

Hebling³

et al. 2014

JMSE-B

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“…There is a consensus on vital tooth bleaching research that HP and its byproducts released from bleaching agents may diffuse through the mineralized dental tissues and reach the pulp space (3,4). In contact with the pulp cells, HP and the ROS resulting from its degradation produce a direct oxidative damage and may generate a secondary oxidative stress due to the imbalance between the amount of ROS and intracellular antioxidants (1).…”

Section: Discussionmentioning

confidence: 99%

“…Clinically, the use of these bleaching gels has been shown to cause postoperative tooth sensitivity (2) and side effects on dental pulp tissue, ranging from acute inflammatory response to the formation of areas of necrosis (3,4), as a result of HP diffusion through the dentinal tubules, reaching the pulp chamber (5) and causing an oxidative stress to pulp cells (6). In view of this, it has been suggested that the application of antioxidant agents prior to bleaching procedures could prevent extracellular and intracellular oxidative damages caused to the pulp cells by HP leached from bleaching gel components (7,8).…”

Section: Introductionmentioning

confidence: 99%

Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide

et al. 2014

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This in vitro study evaluated the potential protective effect of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (HP) applied on dental pulp cells. Odontoblast-like MDPC-23 cells were seeded on 96-well plates for 72 h, treated with different concentrations of α-T (1, 3, 5, and 10 mM) for different times (1, 4, 8, and 24 h) and then exposed or not to a 0.018% HP solution for 30 min. In positive and negative control groups, cells were exposed to HP or culture medium (DMEM containing 5% DMSO), respectively. Cell viability was assessed by the MTT assay and the absorbance numeric data, expressed as percentage values, were subjected to the statistical analysis by KruskalWallis and Mann-Whitney tests (α =5%). Considering the cells in the negative control as having 100% of cell viability, all combinations of α-T concentrations and pretreatment times showed a protective effect against HP cytotoxicity. Significant reduction of cell viability (59%) was observed in the positive control compared with the negative control.The highest values of pulp cell viability were obtained after pretreatment with 1 and 3 mM α-T concentrations for 24 h followed by exposure to HP (126% and 97% of cell viability, respectively). Under the tested conditions, the most effective cell protection against the cytotoxic effects of HP was provided by the lowest concentrations of α-T (1 and 3 mM) applied for 24 h.

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“…Dental tissues that have different levels of pigment saturation are expected to absorb different amounts of peroxide from bleaching products. The confirmation of this supposition would result in great improvements in the development of individualized and safe therapies, avoiding the unwanted penetration of reactive oxygen species into the pulp tissue (13)(14)(15)(16)(17), which has been related to cell damage (11,12) and clinical symptoms (2,18).…”

Section: Introductionmentioning

confidence: 99%

Effect of Dental Pigmentation Intensity on the Transenamel and Transdentinal Penetration of Hydrogen Peroxide

et al. 2016

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This study aimed to evaluate the transenamel and transdentinal penetration of hydrogen peroxide (H 2 0 2 ) applied to bovine teeth pigmented with black tea at different intensities. The following groups were formed DW: immersion in distilled water; BT100: immersion in an infusion of 1.6 g of black tea per 100 mL distilled water; BT10: immersion in an infusion of 1.6 g black tea per 10 mL distilled water. All groups were immersed for 6 days. To quantify the penetration of H 2 0 2 , the specimens were placed in artificial pulp chambers (APCs) and subjected to bleaching treatment with 38% H 2 O 2 once per week for 3 weeks. After bleaching treatment, the acetate buffer solution of APCs with peroxidase enzyme was evaluated in a reflection spectrophotometer. The transenamel and transdentinal penetration of H 2 O 2 and the L* values obtained at T1, T2 and T3 were subjected to Kruskal-Wallis and Friedman statistical tests. At T1, the H 2 O 2 diffusion in DW was higher than that in BT100 and BT10. At the other evaluation times, the penetration values in BT100 and BT10 increased and remained similar. The L* values increased significantly in all groups at T1. At T2, the L* values were higher in DW, while the values in BT100 and BT10 were similar to each other. At the end of the experiment, BT10 showed the lowest L* values. The pigmentation level did not affect the penetration of H 2 O 2 through the enamel and dentin and the bleaching agent effectively changed the color of the teeth.

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The Number of Bleaching Sessions Influences Pulp Tissue Damage in Rat Teeth

Cited by 82 publications

References 26 publications

Histopathological Features of Dental Pulp Tissue from Bleached Mandibular Incisors

Histopathological Features of Dental Pulp Tissue from Bleached Mandibular Incisors

Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide

Effect of Dental Pigmentation Intensity on the Transenamel and Transdentinal Penetration of Hydrogen Peroxide

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