The orphan nuclear receptor NR4A1 attenuates oxidative stress-induced β cells apoptosis via up-regulation of glutathione peroxidase 1

Yang, Yingfeng; Xie, Fangyu; Qin, Dandan; Zong, Chen; Han, Feng; Pu, Ze‐qing; Liu, Dong; Li, Xia; Zhang, Yuchao; Liu, Yuantao; Wang, Xiangdong

doi:10.1016/j.lfs.2018.04.027

Cited by 19 publications

(15 citation statements)

References 20 publications

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“…GPX1 is the important member in the GPX class. Nuclear receptor subfamily 4 group A member 1 (NR4A1) increases GPX1 expression, protecting pancreatic β cells against oxidative stress‐induced apoptosis (Y. Yang et al, 2018). GSS and GPX1 may also be the mechanisms of miR‐423‐5p regulating sperm oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Upregulated microRNA‐423‐5p promotes oxidative stress through targeting glutathione S‐transferase mu 1 in asthenozoospermia

Zhang

Zuo

Cao

2021

Molecular Reproduction Devel

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The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA‐423‐5p (miR‐423‐5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR‐423‐5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n = 40) and normozoospermic (Norm, n = 40) groups. Fresh semen samples were collected and the sperm cells were separated. Quantitative Real‐Time polymerase chain reaction was used to measure the sperm miR‐423‐5p level. Receiver operating characteristic curve (ROC) was employed to test the diagnostic performance of miR‐423‐5p in asthenospermia. Dual‐reporter luciferase assay was adopted to confirm the target gene of miR‐423‐5p. The target gene level in asthenozoospermia and normozoospermia was measured, and the biological function of target gene in asthenozoospermia was evaluated. Results showed that the miR‐423‐5p expression level in the AZS group was higher than that in Norm group, which was positively correlated with the severity of asthenozoospermia. ROC analysis of miR‐423‐5p showed an area under curve (AUC) of 0.69 (95% confidence interval = 0.57–0.80, p <0 .01), with 80% sensitivity and 60% specificity. Glutathione S‐transferase mu 1 (GSTM1) is a target gene of miR‐423‐5p, which significantly decreased in the AZS group. Compared with Norm group, glutathione S‐transferase (GST) activity and total antioxidant capacity (TAC) level decreased, while malondialdehyde (MDA) level increased in the AZS group. Furthermore, GST activity and TAC level were negatively correlated with miR‐423‐5p expression, while MDA level was positively correlated with miR‐423‐5p expression. In conclusion, the sperm miR‐423‐5p level significantly was upregulated in asthenozoospermia. High‐level miR‐423‐5p inhibited sperm motility through targeting GSTM1 to promote oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Upregulated microRNA‐423‐5p promotes oxidative stress through targeting glutathione S‐transferase mu 1 in asthenozoospermia

Zhang

Zuo

Cao

2021

Molecular Reproduction Devel

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“…It is known that pancreatic cell senescence or/and pancreatic cell loss results in diabetes. We further figured out that NR4A1 directly or indirectly enhances the expression of some anti‐apoptotic proteins, such as survivin, WT1, BCL2, SOD1 and GPX1 4,5,42 . These anti‐apoptotic proteins plus some other NR4A1‐up‐regulated molecules might form a protective network to work together to protect pancreatic β cells.…”

Section: Discussionmentioning

confidence: 89%

“…We previously found that NR4A1 was inducible upon treatment with ER stress inducer (TG) or ROS (H 2 O 2 ) in pancreatic β‐cells 4,5,42 . Recently, we found that overexpression of NR4A1 in MIN6 cells resisted the JNK phosphorylation induced by TG or H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

“…Mice genomic DNA was extracted as previously described 42 . Mice cDNA library was obtained from WT C57BL/6J mouse liver with ReverTra Ace qPCR RT Kit (Toyobo).…”

Section: Methodsmentioning

confidence: 99%

“…Mice genomic DNA was extracted as previously described. 42 Mice cDNA library was obtained from WT C57BL/6J mouse liver with ReverTra Ace qPCR RT Kit (Toyobo). We designed a pair of primers with HindIII and XbaI restriction sites at 5′ and 3′ to amplify the cDNA of MKK4 and cloned the PCR product into pFLAG-CMV™-2 vector (Sigma).…”

Section: Plasmid Constructionmentioning

confidence: 99%

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NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β‐cells for protection

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Although the aetiology of type 2 diabetes is complex, it is well accepted that insulin resistance leads to sustained hyperglycaemia or hyperlipidaemia, which consequently results in cellular ER stress and/ or ROS overproduction in pancreatic β-cells, whereas ER stress or ROS causes cell damage or even apoptosis. 1-6 Pancreatic β-cell loss plays an important role in the pathogenesis of type 2 diabetes. To reveal the

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The orphan nuclear receptor NR4A1 attenuates oxidative stress-induced β cells apoptosis via up-regulation of glutathione peroxidase 1

Cited by 19 publications

References 20 publications

Upregulated microRNA‐423‐5p promotes oxidative stress through targeting glutathione S‐transferase mu 1 in asthenozoospermia

Upregulated microRNA‐423‐5p promotes oxidative stress through targeting glutathione S‐transferase mu 1 in asthenozoospermia

NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β‐cells for protection

Acacetin antagonized lipotoxicity in pancreatic β-cells via ameliorating oxidative stress and endoplasmic reticulum stress

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