The pattern of concanavalin a-binding sites during the early development of xenopus laevis

O'Dell, D.S.; Tencer, Renée; Monroy, Alberto; Brachet, Jean

doi:10.1016/0045-6039(74)90001-3

Cited by 44 publications

(2 citation statements)

References 14 publications

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“…6 a-c). This result differs from reports in other species of embryos (O'Dell et al 1974;Currie et al 1984;Smitz-van Prooije et al 1986). It has also been suggested that Con A-binding sites on cell surfaces are involved in establishing the adhesiveness in neurulation of chicks (Lee et al 1976) and in nasal fold fusion of mice (Burk et al 1979).…”

Section: Discussioncontrasting

confidence: 56%

The masking effect of sialic acid on Con A, PNA and SBA ectoderm binding sites during neurulation in the bantam chick embryo

Takahashi

1992

Anat Embryol

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The masking effect of sialic acid on cell surface carbohydrates localized on the ectoderm in stage 6-11 bantam embryos was examined using fluorescein isothiocyanate-labeled Con A, PNA, SBA, LFA, and LPA before and after neuraminidase treatment. The results showed selective lectin binding on both the neuroectoderm and the surface ectoderm. In general, these lectin-binding sites increased or were at least expressed on neuroectoderm during neurulation. On the apical surfaces of the developing neuroectoderm, masked Con A-binding sites were evident from the earliest stage and rapidly increased. These sites coexisted with unmasked binding sites which gradually increased. Masked PNA sites were rarely observed but became abundant in later stages, even though coexistent unmasked sites also rapidly increased. Masked SBA sites were poorly observable in the early stage and gradually increased thereafter, whereas unmasked sites were expressed at later stages. On the basal surfaces masked Con A sites were evident in the early stages but gradually decreased in later stages, whereas unmasked sites were relatively abundant and increased thereafter. Masked PNA sites were evident and increased very rapidly, whereas unmasked sites became observable up to the latest stage. Masked SBA sites were minimal in all three stages, and unmasked sites expressed themselves slightly at later stages. The change in composition of carbohydrates on the developing neuroectoderm was obviously different from that on the developing surface ectoderm. On the contact surface of the neural ridge, the number of masked sites of penultimate sugars was large at Con A sites, slight at PNA and SBA sites, which coexisted with unmasked sugar chain terminals in the areas where Con A sites were moderate and where PNA and SBA sites were poor. Finally, the role of masking on binding sites for Con A, PNA and SBA during neural tube closure is discussed, and the observation that the apparent masking effect on three lectin binding sites did not correspond to the content of sialic acid detected by LFA and LPA is a subject for further study.

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Section: Discussioncontrasting

confidence: 56%

The masking effect of sialic acid on Con A, PNA and SBA ectoderm binding sites during neurulation in the bantam chick embryo

Takahashi

1992

Anat Embryol

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“…Detection of β-catenin protein using immunofluorescence revealed that β-catenin is not enriched at cleavage planes of nebel mutant embryos (Figure 3b). As an additional marker for membrane dynamics at the forming furrow, we tested for binding to the lectin Concanavalin A (Con A), which preferentially binds glycosylated receptors in membranes of cleavage planes in amphibians [42] and in zebrafish (Figure 3c). Con A binding was also reduced in furrow regions of nebel mutant embryos (Figure 3d).…”

Section: Reduced Fma Function In Nebel Mutants Results In Reduced Membrane Deposition At the Furrowmentioning

confidence: 99%

A mutation in the zebrafish maternal-effect gene nebel affects furrow formation and vasa RNA localization

et al. 1999

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Concanavalin A‐binding by cells of the early chick embryo

Hook

Sanders

1977

Journal Cellular Physiology

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The surfaces of cells from the early embryo of the chick were examined using electron microscope techniques for the visualization of concanavalin A-binding sites. Horseradish peroxidase and Ferritin labelled concanavalin A were used to determine the distribution of the binding sites. All surfaces of the epiblast and hypoblast layers which were accessible to concanavalin A showed the presence of binding sites in stage 1 embryos. The ventral surface of the epiblast showed a high lectin affinity which may reflect the development of a basal lamina on this surface. The individual hypoblast cells at this stage showed a non-uniform distribution of binding sites, having a greater affinity on the dorsal surface than the ventral. By the time of primitive streak formation (stage 4-5) the dorsal surface of the epiblast displayed increased binding sites, while the frequency of sites on the ventral surface of the endoblast was reduced. The latter may reflect a change from one cell population to another, which occurs in the lower layer of the embryo at this time. No consistent correlation could be drawn between changes in motility of cells actually invaginating through the primitive streak and changes in affinity for concanavalin A. An overall increase in affinity of the dorsal surface of the epiblast was revealed by Ferritin and may reflect the changes in surface structure occurring in readiness for the morphogenetic migrations of gastrulation.

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The pattern of concanavalin a-binding sites during the early development of xenopus laevis

Cited by 44 publications

References 14 publications

The masking effect of sialic acid on Con A, PNA and SBA ectoderm binding sites during neurulation in the bantam chick embryo

The masking effect of sialic acid on Con A, PNA and SBA ectoderm binding sites during neurulation in the bantam chick embryo

A mutation in the zebrafish maternal-effect gene nebel affects furrow formation and vasa RNA localization

Concanavalin A‐binding by cells of the early chick embryo

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