The probability of parentage exclusion based on restriction fragment length polymorphisms

Itô, Hiroko; Yasuda, Norikazu; Matsumoto, Hideo

doi:10.1007/bf01907963

Cited by 12 publications

(5 citation statements)

References 10 publications

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“…In the past decade, variation in the DNA sequence, mostly in the satellite region, has been exploited to carry out genome individualization (Jeffreys et al 1985;Epplen 1988), which, in turn, has proved to be useful for establishing individuality in forensic (Gill and Werrett 1987;Helminen et al 1988;Kasai et al 1990) and immigration cases (Jeffreys et al 1985;Jeffreys et al 1986), for resolving paternity disputes (Ito et al 1985), and for the analysis of pedigree and genetic disorders. Highly polymorphic markers, such as short tandem repeats (STRs) or microsatellites, have further proved to be useful for this purpose, as they are distributed at many loci and are amenable to automation (Budowle and Moretti 1999).…”

Section: Introductionmentioning

confidence: 99%

Detection of genetic variation in Indian population groups using a novel minisatellite probe and finding relationships through tree construction

Saha

Bamezai

2000

J Hum Genet

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Genetic variation in HaeIII-digested genomic DNA samples from different individuals belonging to population groups from Bengal, Uttar Pradesh (UP), Punjab, and South India was assessed at hypervariable loci, using a minisatellite probe, pBA1.2 (accession number, AF 157691), the repeat unit of which was 24 mer long and rich in G-bases. Comparison of DNA profiles between individuals showed a very low probability of band sharing, which ranged from 0.18 to 0.24. A dendrogram, based on Nei's genetic distance, constructed by the neighbor-joining method, showed the formation of separate clusters by both South Indian and non-Indian samples, whereas the construction of a dendrogram based on the Unweighted pair group method arithmetic average (UPGMA) method with Jaccard's similarity coefficient at the individual level led to the formation of several small clusters which were interleaved; also, the subgroups for each of the populations were intermingled with the subgroups for the other populations. A separate analysis was carried out to check the consistency of the proximity between different individuals forming a cluster and between those individuals who were in the vicinity of two clusters. The dendrograms thus obtained did not change the relationship between the individuals from all the populations studied. Despite the distinct clustering observed in the population group comparison, a probable admixture was reflected in the finding that some individuals belonging to one population group were dispersed or embedded within a cluster generated by the individuals of another population group, when a minute dissection of the data for generating a tree at the individual level was carried out.

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Section: Introductionmentioning

confidence: 99%

Detection of genetic variation in Indian population groups using a novel minisatellite probe and finding relationships through tree construction

Saha

Bamezai

2000

J Hum Genet

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“…Polymorphic information content (PIC) was calculated according to formula of Botstein, White, Skolnick and Davis (1980). The probability of parentage exclusion for each polymorphic locus studied here was calculated by the method of Ito, Yasuda and Matsumoto (1985). Discrimination power and matching probabilities for each locus were determined according to Fisher (1951).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a novel pentaplex microsatellite marker system for paternity studies in Atlantic cod (Gadus morhua L.)

2006

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Five Atlantic cod (Gadus morhua L.) microsatellites (Gmo8, Gmo19, Gmo35, Gmo37 andTch11) were selected for parentage assignment. All markers were co-ampli-¢ed using dye-labelled primers and sized by automated sample processing in a 3130xl Genetic Analyser. The genotyping of samples was reproducible and alleles from all loci could be genotyped con¢dently (precision and accuracy above 99.7 and 99.1% respectively). The number of alleles, observed allelic and genotypic frequencies were calculated. A population database was established for ¢sh originating from randomly selected wild-caught Norwegian coastal-and North-East Arctic cod. Combined paternity exclusion and the probability of match were 99.97 and 1.5 Â 10 À 9 respectively. The multiplex assay system was used successfully for parental assignment of 2336 juveniles from di¡erent mass spawning breeding tanks. Of all o¡spring, 91.2% were assigned to a single parental pair. In total,523 di¡erent matings were recorded, and 69 of 70 dams and all of the 30 sires contributed to the o¡spring.

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“…2. The paternity exclusion was calculated using the formula described for RFLP by Ito et al (1985). The paternity index was calculated according to Garber and Morris (1983).…”

Section: Lc-i Lc-2 Ic-i Ic-2 Ic-3 Ic-4mentioning

confidence: 99%

Investigation of paternity establishing without the putative father using hypervariable DNA probes

et al. 1990

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SummarySeven kinds of DNA probes which recognize hypervariable loci were applied for paternity test. The putative father was decreased and unavailable for the test. The two legitimate children and their mother (the deceased's wife) and the four illegitimate children and their mother (the deceased's kept mistress) were available for analysis. Paternity index of four illegitimate child was investigated. Allelic frequencies and their confidence intervals among unrelated Japanese individuals were previously reported from our laboratory, and co-dominant segregation of the polymorphism was confirmed in family studies. Cumulative paternity indices of four illegitimate children from 16 kinds of standard blood group markers were 165, 42, 0.09, and 36, respectively. On the other hand, cumulative paternity indices from 7 kinds of DNA probes are 2, 363, 4,685, 57,678, and 54,994, respectively, which are 14, 113, 640, 864, and 1,507 times higher than that from standard blood group markers. The DNA analyses gave nearly conclusive evidence that the putative father was the biological father of the children. Especially, the paternity relation of the third illegitimate child could not be established without the DNA analyses. Accordingly, DNA polymorphism is considered to be informative enough for paternity test.

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The probability of parentage exclusion based on restriction fragment length polymorphisms

Cited by 12 publications

References 10 publications

Detection of genetic variation in Indian population groups using a novel minisatellite probe and finding relationships through tree construction

Detection of genetic variation in Indian population groups using a novel minisatellite probe and finding relationships through tree construction

Evaluation of a novel pentaplex microsatellite marker system for paternity studies in Atlantic cod (Gadus morhua L.)

Investigation of paternity establishing without the putative father using hypervariable DNA probes

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