The QRAR model study of β-lactam antibiotics by capillary coated with cell membrane

Characterizations of interaction between antimicrobial peptide (JCpep7) and living staphylococcus aureus used as pseudostationary phase in capillary electrochromatography

“…15,16 Yang et al studied the quantitative retention-activity relationship model of b-lactam antibiotics by coating the cell membrane on the bare-fused capillary via sol-gel technology as pseudo-stationary phase. 17 Nilsson et al used the nanoparticle as pseudostationary phase in Capillary Electrochromatography for protein analysis. 18,19 Up to now, it has not been reported in detail that the living Staphylococcus aureus cells (LSACs) suspensions with a series of concentrations in the running buffer were used as pseudostationary phase to characterize the binding of AMPs to LSACs by Capillary Electrochromatography (CEC).…”

Investigation of interaction between the drug and cell membrane by capillary electrophoresis

“…But this approach has some limitations, for instance, the preparation of CMSP is difficult, CMSP can be easily damaged, and the measuring process is tedious. Yang and coworkers studied the quantitative retention-activity relationships (QRAR) model of β-lactam antibiotics, in which the cell membrane was coated on the bare-fused capillary via sol-gel technology as pseudo-stationary phase [7] . However, the cell membrane was still attached to the support, which might be different from the interaction between drugs and biomolecule in the real blood circulatory system.…”

Liposomes in chromatography

“…Another RP-HPLC method was employed for the measurement of lactone and carboxylate forms of 10-hydroxycamptothecin (HCPT), a promising anticancer agent loaded in bovine serum albumin (BSA) nanoparticles. The results indicated that HCPT-BSA nanoparticles seem to be a appropriate delivery system for the poorly soluble HCPT and its derivatives (Yang et al, 2008). The heat-induced release of tranexamic acid (TA), an antifibrinolytic agent from thermosensitive liposomes, was followed by RP-HPLC using an ODS column.…”

“…It was also concluded from the retention data that the effect of cell membrane coating amount and the pH of the environment markedly influence the retention of the bioactive analytes. Quantitative retentionactivity relationship (QRAR) computations suggested that the retention times determined in cell membrane-coated capillary can be employed in QRAR calculations (Yang et al, 2008).…”