The Role of Anopheles gambiae P450 Cytochrome in Insecticide Resistance and Infection

Félix, Rute C.; Silveira, Henrique

doi:10.5772/29217

Cited by 7 publications

(11 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reactive oxygen species (ROS) are generated by respiration in the mitochondria or as part of the immune response [36]. P450 cytochromes are oxidases acting terminally in monooxygenase systems [37]. Some are regulated in response to infection possibly either as direct immune responders [38], producing nitric oxide (NO) or other reactive oxygen radicals or as part of the host detoxification process decreasing oxidative stress after an infection [36].…”

Section: Resultsmentioning

confidence: 99%

Differential gene expression and alternative splicing in insect immune specificity

Riddell

Garces

Adams

et al. 2014

Preprint

View full text Add to dashboard Cite

Background: Ecological studies routinely show genotype-genotype interactions between insects and their parasites. The mechanisms behind these interactions are not clearly understood. Using the bumblebee Bombus terrestris/trypanosome Crithidia bombi model system (two bumblebee colonies by two Crithidia strains), we have carried out a transcriptome-wide analysis of gene expression and alternative splicing in bees during C. bombi infection. We have performed four analyses, 1) comparing gene expression in infected and non-infected bees 24 hours after infection by Crithidia bombi, 2) comparing expression at 24 and 48 hours after C. bombi infection, 3) determining the differential gene expression associated with the bumblebee-Crithidia genotype-genotype interaction at 24 hours after infection and 4) determining the alternative splicing associated with the bumblebee-Crithidia genotype-genotype interaction at 24 hours post infection. Results:We found a large number of genes differentially regulated related to numerous canonical immune pathways. These genes include receptors, signaling pathways and effectors. We discovered a possible interaction between the peritrophic membrane and the insect immune system in defense against Crithidia. Most interestingly, we found differential expression and alternative splicing of immunoglobulin related genes (Dscam and Twitchin) are associated with the genotype-genotype interactions of the given bumblebee colony and Crithidia strain. Conclusions:In this paper we have shown that the expression and alternative splicing of immune genes is associated with specific interactions between different host and parasite genotypes in this bumblebee/trypanosome model.

show abstract

Section: Resultsmentioning

confidence: 99%

Differential gene expression and alternative splicing in insect immune specificity

Riddell

Garces

Adams

et al. 2014

Preprint

View full text Add to dashboard Cite

show abstract

“…The cytochrome P450s are the primary enzyme family responsible for pyrethroid metabolism in insects and metabolize insecticides through O-, S-, and N-alkyl hydroxylation, aliphatic hydroxylation & epoxidation, aromatic hydroxylation, ester oxidation, nitrogen and thioether oxidation (Wilkinson, 1976;Brogdon and McAllister,1998;Zomuanpuii, 2012). It has been well documented that P450-mediated pyrethroid resistant insects have higher levels or more efficient enzyme forms of one or more P450s compared to susceptible Anopheles gambiae (Feyereisen, 1999;Félix and Silveira, 2012) Corbel and N'Guessan, 2013).…”

Section: Insecticide Resistance In Anopheles Gambiaementioning

confidence: 99%

“…Cytochrome P450s are designated various names, including cytochrome P450 monooxygenases, polysubstrate monooxygenases (PSMOs), microsomal oxidizes, heme-thiolate proteins and mixed function oxidases (MFOs) (Omura and Sato, 1962;Fatma and Wajidi, 2013). Cytochrome P450s are organised into families (those with >40% amino acid sequence homology are usually grouped into the same family) and subfamilies (those with >55% amino acids sequence homology) (Félix and Silveira, 2012;Nakamura et al, 2012). Gene family numbers are prefixed by the word "CYP"…”

Section: Nomenclaturementioning

confidence: 99%

Regulatory mechanisms involved in the control of CYP6M2 gene in insecticide resistant in Anopheles gambiae (Diptera: Culicidae)

Mohammed

2014

Journal of Biotechnology

View full text Add to dashboard Cite

Cytochrome P450s, including CYP6M2 gene, are involved in the detoxification of permethrin. Some of these genes are regulated by CnCC / dKeap 1 and Spineless / Tango in Drosophila melanogaster. This mechanism is yet to be identified in Anopheles gambiae. Thus, we examine whether there is differential regulation of CYP6M2 gene between permethrin resistant Tiassalé and susceptible Kisumu strains of An. gambiae. Bioinformatics analysis was used to search for cis-acting elements within CYP6M2 (896 bp) region hypothesised to contain the promoter. Isolated and cloned CYP6M2 promoter reporter constructs were transfected into Anopheles gambiae Sua 5.1* cells to measure luciferase activity as a surrogate promoter activity. The WHO adult bioassay was used to expose adult females of the permethrin resistant Tiassalé and susceptible Kisumu strains of An. gambiae to discriminating doses of 0.75% permethrin. Uncharacterised strains from Auyo (Auyo-Nigeria) selected to 4% DDT and 0.1% Regulatory Mechanisms Involved in the Control of CYP6M2

show abstract

“…Cyp12a4 (substrate-Lufeneron) and Cyp12d1 (substrate-DDT and carbaryl) [12][13][14]. In addition, P450s also partake in the biosynthesis of cuticular hydrocarbons, ecdysteroids, juvenile hormone, and pheromones [15,16]. In higher mammals, some of these xenobiotic metabolizing cytochrome P450 genes are established to be upregulated by the transcription factors such as the Aryl hydrocarbon receptor (AhR)/Aryl hydrocarbon receptor nuclear translocator (ARNT) and/or Nuclear factor erythroid-2 related factor-2 (Nrf2)/Kelchlike ECH-associated protein 1 (Keap 1) [17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Understanding the Mechanisms Involved in the Regulation of Cytochrome P450 Gene Expression in Drosophila melanogaster (Diptera: Drosophilidae)

Br¹,

Simon²,

Mn³

et al. 2016

Entomol Ornithol Herpetol

View full text Add to dashboard Cite

The Role of Anopheles gambiae P450 Cytochrome in Insecticide Resistance and Infection

Cited by 7 publications

References 58 publications

Differential gene expression and alternative splicing in insect immune specificity

Differential gene expression and alternative splicing in insect immune specificity

Regulatory mechanisms involved in the control of CYP6M2 gene in insecticide resistant in Anopheles gambiae (Diptera: Culicidae)

Understanding the Mechanisms Involved in the Regulation of Cytochrome P450 Gene Expression in Drosophila melanogaster (Diptera: Drosophilidae)

Contact Info

Product

Resources

About