The Role of Genetic Variation in Maize Response to Beneficial Endophytes

Schultz, Corey Robert; Brantley, Kamaya M.; Wallace, Jason

doi:10.1101/2021.11.03.467096

Cited by 3 publications

(3 citation statements)

References 49 publications

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“…Maize lines B73, Mo17, and their F1 hybrid seeds were sterilized via our previously established method [65]. Seeds were surface sterilized with sterile water, bleach, and tween 20 and then placed in a hot water bath.…”

Section: Methodsmentioning

confidence: 99%

Effects of Inbreeding on Microbial Community Diversity ofZea mays

Schultz¹,

Johnson²,

Wallace³

2023

Preprint

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Heterosis, also known as hybrid vigor, is the basis of modern maize production. The effect of heterosis on maize phenotypes has been studied for decades, but its effect on the maize-associated microbiome is much less characterized. To determine the effect of heterosis on the maize microbiome, we sequenced and compared the bacterial communities of inbred, open pollinated, and hybrid maize. Samples covered three tissue types (Stalk, Root, and Rhizosphere) in two field experiments and one greenhouse experiment. Bacterial diversity was affected by location and tissue type, but not genetic background, for both within-sample (alpha) and between-sample (beta) diversity. PERMANOVA analysis similarly showed that tissue type and location had significant effects on the overall community structure, whereas the genetic background and individual plant genotypes did not. Differential abundance analysis identified only 18 bacterial ASVs that significantly differed between inbred and hybrid maize. Predicted metagenome content was inferred with Picrust2, and it also showed a significantly larger effect of tissue and location than genetic background. Overall, these results indicate that the bacterial communities of inbred and hybrid maize are often more similar than they are different, and that non-genetic effects are generally the largest influences on the maize microbiome.

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Section: Methodsmentioning

confidence: 99%

Effects of Inbreeding on Microbial Community Diversity ofZea mays

Schultz¹,

Johnson²,

Wallace³

2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Maize lines B73 and Mo17 and their F1 hybrid seeds were sterilized via our previously established method [70]. Seeds were surface-sterilized with sterile water, bleach, and Tween 20, then placed in a hot water bath.…”

Section: Minimaize Inoculation Experimentsmentioning

confidence: 99%

Effects of Inbreeding on Microbial Community Diversity of Zea mays

2023

Self Cite

View full text Add to dashboard Cite

Heterosis, also known as hybrid vigor, is the basis of modern maize production. The effect of heterosis on maize phenotypes has been studied for decades, but its effect on the maize-associated microbiome is much less characterized. To determine the effect of heterosis on the maize microbiome, we sequenced and compared the bacterial communities of inbred, open pollinated, and hybrid maize. Samples covered three tissue types (stalk, root, and rhizosphere) in two field experiments and one greenhouse experiment. Bacterial diversity was more affected by location and tissue type than genetic background for both within-sample (alpha) and between-sample (beta) diversity. PERMANOVA analysis similarly showed that tissue type and location had significant effects on the overall community structure, whereas the intraspecies genetic background and individual plant genotypes did not. Differential abundance analysis identified only 25 bacterial ASVs that significantly differed between inbred and hybrid maize. Predicted metagenome content was inferred with Picrust2, and it also showed a significantly larger effect of tissue and location than genetic background. Overall, these results indicate that the bacterial communities of inbred and hybrid maize are often more similar than they are different and that non-genetic effects are generally the largest influences on the maize microbiome.

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“…Plant genetics impact interactions with both individual beneficial microorganisms [29][30][31][32], as well as the overall microbiome community [3,12,[33][34][35][36]. The environment provides most of the microbes for community recruitment (outside of seed endophytes [35]), and it has a direct impact on soil [37], plants [38], and maize [39] microbial community structure.…”

Section: Introductionmentioning

confidence: 99%

The Landscape of Maize-Associated Bacteria and Fungi Across the United States

Schultz¹,

Desai²,

Wallace³

2023

Preprint

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The maize microbiome consists of microbes that are associated with plants, and can be shaped by the host plant, the environment, and microbial partners, some of which can impact plant performance. We used a public dataset to analyze bacteria and fungi in the soil, rhizosphere, roots, and leaves of commercial maize at 30 locations across the US. We found that both tissue type and location had significant effects on community structure and makeup, although the patterns differed in bacteria and fungi based on tissue type. We also found many differences in predicted microbial gene pathways between tissues, with location also shaping predicted functional gene profiles. We found a pattern of potential interaction between fungi and bacteria, and potential intra-kingdom mutualism, in microbiome networks. The robustness of these networks was dependent upon tissue, with endophytes in leaves and roots showing significantly higher natural connectivity. Within a tissue, this connectivity was relatively stable across locations. We identified environment and soil characteristics that may impact tissue specific microbial abundance. Sulfate level in the soil was positively correlated with Proteobacteria abundance, but negatively correlated with Firmicutes abundance in the roots and leafs. Ascomycota appears to be affected by different environmental variables in each tissue. We also identified gene functions and enzymes which may be necessary to allow microbes to transition across compartments and become endophytes.

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The Role of Genetic Variation in Maize Response to Beneficial Endophytes

Cited by 3 publications

References 49 publications

Effects of Inbreeding on Microbial Community Diversity ofZea mays

Effects of Inbreeding on Microbial Community Diversity ofZea mays

Effects of Inbreeding on Microbial Community Diversity of Zea mays

The Landscape of Maize-Associated Bacteria and Fungi Across the United States

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