Scalar mixing in turbulent flows is widely observed in nature as well as in industrial plants. In this chapter, we deal with three topics related to mixing and diffusion in grid-generated turbulence. The first topic (shown in Sect. 1) is experimental research on an axisymmetric CO 2 jet issuing into free-stream turbulent flows generated by a square-mesh biplane round-rod grid (referred to as a regular grid in Sect. 1) and a square fractal grid. The CO 2 jet issues from a small pipe located in the decaying region of these grid turbulences. A composite probe consisting of two concentration-sensitive I-type hot-wire sensors is used. For both flows, the mesh Reynolds number in the free stream is 6,000, and the jet Reynolds number based on the relative velocity between the free stream and the exit velocity of the jet is 5,000. The Taylor Reynolds numbers are about 100 and 35 for the square fractal grid and the regular grid, respectively. The results show that the half-widths of the mean velocity and concentration of the jets increase more rapidly, and the root mean square velocity and concentration in the axial direction decay more slowly for stronger free-stream turbulence. The second topic (shown in Sect. 2) is the development of a mixing layer of a high-Schmidt-number passive scalar in turbulent flows generated by a square-mesh biplane square-bar grid (referred to as a regular grid in Sect. 2) and a square fractal grid with the same mesh Reynolds number of 2500. A uniform passive scalar (Rhodamine B) is supplied only from the lower stream; therefore, scalar mixing layers with an initial step profile develop downstream of the grids. Particle image velocimetry and planar laser-induced fluorescence are used to investigate the