The Soybean Purple Acid Phosphatase GmPAP14 Predominantly Enhances External Phytate Utilization in Plants

Kong, Youbin; Li, Xihuan; Wang, Bing; Li, Wenlong; Du, Hui; Zhang, Caiying

doi:10.3389/fpls.2018.00292

Cited by 52 publications

(36 citation statements)

References 53 publications

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“…Then, the roots were harvested for GUS staining after 12 days. The GUS staining was performed described as (Kong et al, 2018). The samples were incubated in 2 mL tube at 37°C for 3 h in GUS staining buffer (2 mM 5-bromo-4-chloro-3-indolyl-bglucuronic acid in 50 mM sodium Pi buffer, pH 7.2) containing 0.1% Triton X-100, 2 mM K 4 Fe(CN) 6 , 2 mM K 3 Fe(CN) 6 , and 10 mM EDTA).…”

Section: Methodsmentioning

confidence: 99%

GmEXLB1, a Soybean Expansin-Like B Gene, Alters Root Architecture to Improve Phosphorus Acquisition in Arabidopsis

Kong

Wang

et al. 2019

Front. Plant Sci.

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Expansins comprise four subfamilies, α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA), and expansin-like B (EXLB), which are involved in the regulation of root development and growth under abiotic stress. To date, few EXLB genes have been shown to respond to low phosphorus (P) in plants. In this study, we identified an EXLB gene, GmEXLB1 , by analyzing the transcription profiles of GmEXLBs in soybean. Quantitative analysis showed that GmEXLB1 was expressed and induced in the lateral roots of soybean under low P conditions. The observation of β-glucuronidase staining in transgenic Arabidopsis suggested that GmEXLB1 might be associated with lateral root emergence. GmEXLB1 overexpression altered the root architecture of transgenic Arabidopsis by increasing the number and length of lateral roots and the length of primary roots under low P conditions. Additionally, the length of the elongation zone and the average cell length in the elongation zone were increased in transgenic Arabidopsis . Increases in biomass and P content suggested that GmEXLB1 overexpression enhanced P acquisition in Arabidopsis . Overall, we conclude that GmEXLB1 expression is induced in soybean under low P conditions, and the overexpression of GmEXLB1 improves P acquisition by regulating root elongation and architecture in Arabidopsis , which provides a possible direction for research of the function of this gene in soybean.

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Section: Methodsmentioning

confidence: 99%

GmEXLB1, a Soybean Expansin-Like B Gene, Alters Root Architecture to Improve Phosphorus Acquisition in Arabidopsis

Kong

Wang

et al. 2019

Front. Plant Sci.

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“…Pi starvation inducible (PSI) extracellular APases are involved in Pi scavenging and hydrolysis from external phosphoesters or organic P, whereas intracellular APases remobilize and recycle Pi from its compound in cytoplasm and organelles ( Veljanovski et al., 2006 ; Tran et al., 2010a ; Wang et al., 2014 ; Zhang et al., 2014 ; Wang and Liu, 2018 ). Several PSI APases have been biochemically and molecularly characterized in some plant species such as Arabidopsis thaliana ( Veljanovski et al., 2006 ; Lohrasebi et al., 2007 ; Kuang et al., 2009 ; Tran et al., 2010b ; Wang et al., 2011 ; Zamani et al., 2014 ; Liu et al., 2016 ; Bhadouria et al., 2017 ; Kong et al., 2018 ; Sabet et al., 2018 ). Among APases, purple acid phosphatases (PAPs) are the most important class of plant PSI APases which appear purple or pink color in water solution due to a bimetallic active center ( Olczak et al., 2003 ; Tran et al., 2010a ).…”

Section: Introductionmentioning

confidence: 99%

The Critical Role of AtPAP17 and AtPAP26 Genes in Arabidopsis Phosphate Compensation Network

et al. 2020

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Purple acid phosphatases (PAP)-encoding genes form a complex network that play a critical role in plant phosphate (Pi) homeostasis. Mostly, the functions of PAPs were investigated individually. However, the interactions of most of these genes in response to various concentrations of available Pi remain unknown. In this study, the roles of AtPAP17 and AtPAP26 genes, and their relationship within Pi homeostasis context were investigated. Surprisingly, atpap17 and atpap26 mutants not only showed no obvious developmental defects, but also produced higher biomass in compare to wild type (WT) plants under normal growth conditions. Comparing gene expression patterns of these mutants with WT plant, we identified a set of genes up-regulated in mutant plants but not in WT. Based on these unexpected results and up-regulation of AtPAP17 and AtPAP26 genes by the loss of function of each other, the hypothesis of compensation relationship between these genes in Pi homeostasis was assessed by generating atpap17/atpap26 double mutants. Observation of developmental defects in atpap17/atpap26 mutant but not in single mutants indicated a compensation relationship between AtPAP17 and AtPAP26 genes in Pi homeostasis network. Taken together, these results demonstrate the activation of AtPAP17 and AtPAP26 genes to buffer against the loss of function of each other, and this compensation relationship is vital for Arabidopsis growth and development.

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“…Induction and secretion of acid phosphatases (APases) is considered to be an important strategy for improving plant growth under conditions of low inorganic phosphate. PAPs, are an important class of plant APases that could be secreted into the rhizosphere to utilize organic phosphorus for plant growth and development [47]. Among the P-related genes, Glyma.17G172700 and Glyma.19G193900 were annotated as purple acid phosphatase.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Long Non-Coding RNAs (lncRNAs) in Two Contrasting Soybean Genotypes Subjected to Phosphate Starvation

zhang

Zhang

Yang

et al. 2020

Preprint

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Background: Phosphorus (P) is essential for plant growth and development, and low-phosphorus (LP) stress is a major factor limiting the growth and yield of soybean. Recently, long noncoding RNAs (lncRNAs) have been reported to be key regulators in response to stress conditions in plants. In soybean, however, how LP stress mediates biogenesis of lncRNAs remains unclear. Results: In this study, to explore the response mechanisms of lncRNAs to LP stress, we used the roots of two representative soybean genotypes with opposite P deficiency responsiveness, a P-sensitive genotype (Bogao) and a P-tolerant genotype (NN94156), to construct RNA sequencing (RNA-seq) libraries. In total, 4,166 novel lncRNAs including 525 differently expressed (DE) lncRNAs were identified across two genotypes at different P levels. GO and KEGG analyses indicated that numerous DE lncRNAs might be involved in diverse biological processes of phosphate, such as lipid metabolic process, catalytic activity, cell membrane formation, signal transduction, nitrogen fixation. Moreover, lncRNA-mRNA-miRNA and lncRNA-mRNA networks were constructed and several promising lncRNAs were identified, which may have highly valuable for further analysis the mechanism in response to LP stress in soybean.Conclusions: These results revealed that LP stress can significantly alter the genome-wide profiles of lncRNAs, especially for P sensitive genotype Bogao. Our findings increase the understanding and provides new insights into the function of lncNRAs responses to P stress in soybean.

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The Soybean Purple Acid Phosphatase GmPAP14 Predominantly Enhances External Phytate Utilization in Plants

Cited by 52 publications

References 53 publications

GmEXLB1, a Soybean Expansin-Like B Gene, Alters Root Architecture to Improve Phosphorus Acquisition in Arabidopsis

GmEXLB1, a Soybean Expansin-Like B Gene, Alters Root Architecture to Improve Phosphorus Acquisition in Arabidopsis

The Critical Role of AtPAP17 and AtPAP26 Genes in Arabidopsis Phosphate Compensation Network

Genome-Wide Analysis of Long Non-Coding RNAs (lncRNAs) in Two Contrasting Soybean Genotypes Subjected to Phosphate Starvation

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