The stress response protein Hsp12p increases the flexibility of the yeast Saccharomyces cerevisiae cell wall

Karreman, Robert J.; Dague, Étienne; Gaboriaud, Fabien; Quilès, Fabienne; Duval, Jérôme F. L.; Lindsey, George G.

doi:10.1016/j.bbapap.2006.10.009

Cited by 50 publications

(43 citation statements)

References 33 publications

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“…However, no transcriptomic data are yet available in public databases (i.e., Gene Expression Omnibus at http://www.ncbi.nlm.nih.gov/gds) to support that these GPI-linked protein-encoding genes are under the control of Msn2/Msn4. Another potential target of ethanol is the plasma membrane protein Hsp12, which was shown to be impli-cated in cell wall elasticity (95) and which is transcriptionally regulated by Msn2/Msn4 (96).…”

Section: Discussionmentioning

confidence: 99%

Evidence for a Role for the Plasma Membrane in the Nanomechanical Properties of the Cell Wall as Revealed by an Atomic Force Microscopy Study of the Response of Saccharomyces cerevisiae to Ethanol Stress

Schiavone

Formosa-Dague

Elsztein

et al. 2016

Appl Environ Microbiol

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A wealth of biochemical and molecular data have been reported regarding ethanol toxicity in the yeast Saccharomyces cerevisiae. However, direct physical data on the effects of ethanol stress on yeast cells are almost nonexistent. This lack of information can now be addressed by using atomic force microscopy (AFM) technology. In this report, we show that the stiffness of glucosegrown yeast cells challenged with 9% (vol/vol) ethanol for 5 h was dramatically reduced, as shown by a 5-fold drop of Young's modulus. Quite unexpectedly, a mutant deficient in the Msn2/Msn4 transcription factor, which is known to mediate the ethanol stress response, exhibited a low level of stiffness similar to that of ethanol-treated wild-type cells. Reciprocally, the stiffness of yeast cells overexpressing MSN2 was about 35% higher than that of the wild type but was nevertheless reduced 3-to 4-fold upon exposure to ethanol. Based on these and other data presented herein, we postulated that the effect of ethanol on cell stiffness may not be mediated through Msn2/Msn4, even though this transcription factor appears to be a determinant in the nanomechanical properties of the cell wall. On the other hand, we found that as with ethanol, the treatment of yeast with the antifungal amphotericin B caused a significant reduction of cell wall stiffness. Since both this drug and ethanol are known to alter, albeit by different means, the fluidity and structure of the plasma membrane, these data led to the proposition that the cell membrane contributes to the biophysical properties of yeast cells. IMPORTANCEEthanol is the main product of yeast fermentation but is also a toxic compound for this process. Understanding the mechanism of this toxicity is of great importance for industrial applications. While most research has focused on genomic studies of ethanol tolerance, we investigated the effects of ethanol at the biophysical level and found that ethanol causes a strong reduction of the cell wall rigidity (or stiffness). We ascribed this effect to the action of ethanol perturbing the cell membrane integrity and hence proposed that the cell membrane contributes to the cell wall nanomechanical properties. T he yeast Saccharomyces cerevisiae is a remarkable ethanol producer that is also very sensitive to its main fermentative product. At low to moderate concentrations (5 to 7%), ethanol mainly affects the growth rate, and at higher concentrations (Ͼ10%), it can strongly impair cell integrity, eventually leading to cell death with features of apoptosis (1). These inhibitory and toxic effects are ascribed to the fact that ethanol alters cell membrane fluidity and dissipates the transmembrane electrochemical potential, thereby creating permeability to ionic species and causing leakage of metabolites (2). Recent works using lipidomic methodologies confirmed the relationship between the composition of lipids, notably ergosterol and unsaturated fatty acids, and ethanol tolerance (3, 4). Moreover, as it diffuses freely into cells, ethanol at high concen...

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Section: Discussionmentioning

confidence: 99%

Evidence for a Role for the Plasma Membrane in the Nanomechanical Properties of the Cell Wall as Revealed by an Atomic Force Microscopy Study of the Response of Saccharomyces cerevisiae to Ethanol Stress

Schiavone

Formosa-Dague

Elsztein

et al. 2016

Appl Environ Microbiol

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“…[29] On the other hand, free hydroxyl groups and carboxyl groups were absorbed at regions (2927.7 cm -1 ) and (2922.0 cm-1) that found in the carbohydrate. [30] Results indicated that the FT-IR spectra of the extracted glucan has appearance typical to that of the standard β-glucan with high degree of purity and absence of the protein contents that absorbed at 1635, 1542, 1650 cm -1 .…”

Section: Figure 2(a): Ft-ir Analysis For Extracted P Eryngii β-Glucanmentioning

confidence: 94%

Anti-Angiogenic Effect of Beta-Glucan Extracted From Pleurotus Spp.

Salim¹

2017

WJPR

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“…This method has been extensively used in recent years for the study of microbial surfaces, and the identification and classification of microorganisms [20][21][22][23].…”

Section: Abstract: Ft-ir Spectroscopy • Lithium • Saccharomyces Cerevmentioning

confidence: 99%

Li+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy

2013

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+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy IntroductionThe capacity of metal cations to induce competence of yeast and bacterial cells to exogenous DNA is the basis of biological methods in biotechnology and molecular genetics [1][2][3]. The most commonly used, efficient protocol, for genetic transformation of Saccharomyces cerevisiae is treatment with alkali metal ions. Li + ions are the most effective of all the cations tested, and the transformation efficiency is comparable to levels obtained by the protoplast method [4]. Li + ions enhance the transformation of intact cells, but no effect is observed on transformation of protoplasts, implying that Li + ions facilitate DNA passage through the cell wall [5]. The mechanism underlying S. cerevisiae transformation includes DNA attachment and penetration through the cell wall, although how DNA passes through the cell wall is not yet clear [6][7][8]. Cell wall density, thickness and structure are factors of major importance during penetration of exogenous molecules into the cell. However, despite the extensive use of Li + ions in yeast transformation protocols, the influence of these cations on the structure of the yeast cell wall has not been widely investigated and, therefore, remains unclear. The influence of Li + ions on the cell surface topography of intact S. cerevisiae cells was observed by atomic force microscopy and it was found that the surface of Li + treated yeast cells became much rougher [9].The S. cerevisiae cell wall is composed of three major components: β-glucans, chitin, and mannoproteins. Glucose residues are linked to other glucose molecules through β(1→3) and β(1→6) linkages and to N-acetylglucosamine via β(1→4) bonds [10,11]. S. cerevisiae mannan has a linear α(1→6)-linked Keywords: FT-IR spectroscopy • Lithium • Saccharomyces cerevisiae • TransformationAbstract: The effect of Li + ions as a transformation inducing agent on the yeast cell wall has been studied. Two Saccharomyces cerevisiae strains, p63-DC5 with a native cell wall, and strain XCY42-30D(mnn1) which contains structural changes in the mannan-protein complex, were used. Fourier transform infrared (FT-IR) spectroscopy has been used for the characterization of the yeast strains and for determination of the effect of lithium cations on the cell wall. A comparison of the carbohydrate absorption band positions in the 970-1185 cm -1 range, of Na + and Li + treated yeast cells has been estimated. Absorption band positions of the cell wall carbohydrates of p63-DC5 were not influenced by the studied ions. On the contrary, the treatment of XCY42-30D(mnn1) cells with Li + ions shifted glucan band positions, implying that the cell wall structure of strain XCY42-30D(mnn1) is more sensitive to Li + ion treatment. backbone with side chains of α(1→2)-and α(1→3)-linked mannose units. In S. cerevisiae, mannoproteins contribute to the regulation of cell wall porosity, and therefore control both the exit of secretary proteins and the entrance of ma...

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The stress response protein Hsp12p increases the flexibility of the yeast Saccharomyces cerevisiae cell wall

Cited by 50 publications

References 33 publications

Evidence for a Role for the Plasma Membrane in the Nanomechanical Properties of the Cell Wall as Revealed by an Atomic Force Microscopy Study of the Response of Saccharomyces cerevisiae to Ethanol Stress

Evidence for a Role for the Plasma Membrane in the Nanomechanical Properties of the Cell Wall as Revealed by an Atomic Force Microscopy Study of the Response of Saccharomyces cerevisiae to Ethanol Stress

Anti-Angiogenic Effect of Beta-Glucan Extracted From Pleurotus Spp.

Li+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy

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