The structure of replicating bacteriophage T1 DNA: Comparison between wild type and DNA-arrest mutant infections

Pugh, J C; Ritchie, Donald A.

doi:10.1016/0042-6822(84)90129-6

Cited by 9 publications

(2 citation statements)

References 32 publications

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“…It thus appears that the 64,500-Da early protein might play an essential role in the formation of the concatemeric DNA intermediate, like the products of genes 6 of T3 (8), 3.5 and 4 of Ti (7), 46 and 47 of T4 (15), and 6 of T7 (11). The products of all of these genes have exonuclease activity (14)(15)(16)23). However, whether these gene products are directly responsible for concatemer formation or for just stabilizing the concatemeric structures is not known.…”

Section: Discussionmentioning

confidence: 99%

Replication and packaging of choleraphage phi 149 DNA

Chowdhury

Ray

et al. 1987

J Virol

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The intracellular replication of the circularly permuted DNA of choleraphage +149 involves a concatemeric DNA structure with a size equivalent to six genome lengths. The synthesis of both monomeric and concatemeric DNAs during replication of +149 occurred in the cytoplasm. The concatemers served as the substrate for the synthesis of mature phage DNA, which was eventually packaged by a headful mechanism starting from a unique pac site in the concatemeric DNA. Packaging of DNA into phage heads involved binding of concatemeric DNA to the cell membrane. A scheme involving sequential packaging of five headfuls proceeding in the counterclockwise direction from the pac site is proposed. After infection under high-phosphate conditions, the concatemeric DNA intermediates were not formed, although synthesis of monomeric molecules was unaffected.

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Section: Discussionmentioning

confidence: 99%

Replication and packaging of choleraphage phi 149 DNA

Chowdhury

Ray

et al. 1987

J Virol

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“…We mention only a few examples. Phage T1 appears to require an origin-dependent and a recombination-dependent pathway for the same reason as T4 [46]. Phage lambda can use different origin initiation pathways [47].…”

Section: Z 1 In Praise Of Redundancymentioning

confidence: 99%

Multiple initiation mechanisms adapt phage T4 DNA replication to physiological changes during T4's development

Mosig¹

1995

FEMS Microbiology Reviews

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We summarize the evidence for multiple pathways to initiate phage T4 DNA replication. In any infecting chromosome, leading DNA strands can be primed from pre-replicative transcripts, independent of primase activity, at one of several origins. Within each origin region, there are multiple RNA-DNA transition sites. However, the priming potential at each single site is very low. Our results suggest that origin transcripts can become primers for leading strand DNA synthesis without being processed, but that a promoter-proximal segment of each origin transcript plays an important structural role, as a proposed wedge, in the transition from RNA to DNA synthesis. Two recombination-dependent pathways render subsequent phage T4 DNA replication independent of transcription. The first of these requires proteins that are synthesized during the pre-replicative phase of infection. It is active as soon as the first growing points, initiated at origins, have reached a chromosomal end. The other one requires at least one late protein: endonuclease VII, a resolvase that cuts recombinational junctions. The latter pathway can bypass primase deficiencies by allowing retrograde DNA synthesis without Okazaki pieces. We discuss the integration of these multiple and redundant pathways into the developmental program of T4. Competition between these initiation mechanisms and with other DNA transactions allows for integration of replication controls with transcription, recombination and packaging of the DNA.

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Bacteriophage T1

Drexler

1988

The Bacteriophages

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The structure of replicating bacteriophage T1 DNA: Comparison between wild type and DNA-arrest mutant infections

Cited by 9 publications

References 32 publications

Replication and packaging of choleraphage phi 149 DNA

Replication and packaging of choleraphage phi 149 DNA

Multiple initiation mechanisms adapt phage T4 DNA replication to physiological changes during T4's development

Bacteriophage T1

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