The Trichophyton Mentagrophytes and Microsporum Canis Infection of the Guinea-Pig

Goss, William A.; Actor, Paul; Jambor, William P.; Pagano, Joseph F.

doi:10.1038/jid.1963.153

Cited by 10 publications

(5 citation statements)

References 7 publications

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“…In addition, in our preliminary experiment using guinea pigs in which no immunosuppression procedure was used, fungal infection in the nail plate and/or bed was not detected by histological examination. This result is in line with previous experimental studies indicating that immunosuppression might be essential for deep fungal invasion 12,13) . Further, rabbits, which are classified in a group of mediumsized experimental animals, can be somewhat difficult to handle and prepare in large numbers, and the nails of these animals may become fragile as a result of the fungal infection.…”

Section: Discussionsupporting

confidence: 93%

Development of an Animal Model of Onychomycosis in Guinea Pigs

Hasegawa

Shibuya

2020

Medical Mycology Journal

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Onychomycosis is a common and intractable superficial mycosis that occurs worldwide. Treatment with both oral and topical drugs is recommended, but the objective evaluation procedure to determine the efficacy of and the appropriate delivery system for the drugs remains controversial. This may be attributed to the lack of a reliable animal model that not only mirrors the pathophysiology of human onychomycosis but is also feasible. Therefore, we attempted to establish an animal model of onychomycosis using immunosuppressed guinea pigs and elucidate the pathophysiology of human onychomycosis. In the present study, we applied Trichophyton mentagrophytes TIMM2789 to the hind limb nails of corticosteroid-treated guinea pigs. The nails were examined macroscopically and histopathologically at 0, 14, and 42 days after a 2-week exposure period to the fungus. A large portion of the experimentally infected nails showed discoloration, which is an important clinical sign, and most infections were confirmed histopathologically in the deep layer of the nail plate at all time points. The infection rates at 0, 14, and 42 days after exposure were 39%, 61%, and 78%, respectively. Thus, we established an animal model of onychomycosis with good reproducibility and that might be appropriate for extrapolation to the pathophysiology of the human disease.

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Section: Discussionsupporting

confidence: 93%

Development of an Animal Model of Onychomycosis in Guinea Pigs

Hasegawa

Shibuya

2020

Medical Mycology Journal

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“…Underlying disease such as Cushing's syndrome, or adrenal hypercorticism (Canizares et al, 1959;Nelson and McNiece, 1959;Pillsbury and Jacobson, 1955), malignant lymphoma (Levene, 1973;Lewis et al, 1953), rheumatoid arthritis (Blank and Smith, 1960), or diabetes (Mandel, 1960), as well as treatment with immunosuppressive agents (Canizares et al, 1959;Lavalle, 1975;Burgoon et al, 1974;Marks and Dawber, 1972;Goss et al, 1963), has been associated with widespread intractable infections. Graham (1972) suggested that T. rubrum should also be considered as an opportunistic organism which takes advantage of a wide spectrum of systemic illnesses, in some instances masking the underlying disease that has altered the host-parasite relationship.…”

Section: Natural Factorsmentioning

confidence: 97%

“…Their patient had received longterm systemic treatment with steroids. Goss et al (1963) showed that widespread dermatophyte infections could also be produced in guinea pigs given very high levels of steroids.…”

Section: Natural Factorsmentioning

confidence: 98%

Immunology of Surface Fungi Dermatophytes

Grappel

1981

Immunology of Human Infection

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“…One of the fungus-infected guinea-pigs (and one of the non-infected control pigs) received regular doses of triamcinolone acetonide beginning 4 days after infection, in an attempt to produce a chronic fungal infection as described by Gross, Actor, Jambor & Pagano (1963).…”

Section: Investigation Of Organismsmentioning

confidence: 99%

Dermatophyte lesions in the hedgehog as a reservoir of penicillin-resistant staphylococci

Smith

Marples

1965

J. Hyg.

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An antibiotic substance biologically resembling penicillin G was produced by the growth ofTrichophyton mentagrophytesvar.erinacei, T. mentagrophytes var. granulareandT. mentagrophytesvar.interdigitalein Sabouraud's dextrose broth. An antibiotic concentration equivalent to 7 units/ml. penicillin G could be produced by var.erinaceiwhen grown in a suitable nutrient medium.Epidermophyton floccosumalso produced a substance which inhibited the growth of the Oxford staphylococcus. However, this substance was not completely inactivated by penicillinase. Noin vitroantibiotic production could be demonstrated withT. rubrum, T. concentricum, T. terrestreorMicrosporum canis.Penicillin production could be demonstrated in rabbit skin infected with T. ment-agrophytes var. erinacei. Pathogenic staphylococci falling on a var. erinacei guinea-pig lesion increased rapidly in the ringworm tissue. The inhibiting concentration of penicillin G for such staphylococci was shown to increase from 5·0 µg./ml. to above 20·0 µg./ml. as the ringworm progressed.The presence ofT. mentagrophytesvar.erinaceiin a high percentage of hedgehogs is considered the main reason why the skin is the primary site of staphylococcal multiplication in hedgehogs and why most of theStaphylococcias aureusstrains recoverable from these animals are penicillin resistant.

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The Trichophyton Mentagrophytes and Microsporum Canis Infection of the Guinea-Pig

Cited by 10 publications

References 7 publications

Development of an Animal Model of Onychomycosis in Guinea Pigs

Development of an Animal Model of Onychomycosis in Guinea Pigs

Immunology of Surface Fungi Dermatophytes

Dermatophyte lesions in the hedgehog as a reservoir of penicillin-resistant staphylococci

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