INTRODUCTIONIn the previous paper (Part III) the use of techniques which assume certain constituents in forage to be indigestible was discussed. These, together with the feed : faecal ratio techniques suffer from the main disadvantage that they require the sampling of a sward for 'herbage as grazed', which is a subjective operation. They also assume 100 per cent recovery in the faeces of herbage constituents such as lignin or ehromogen, while particular care must be taken in obtaining representative samples of faeces in the field.However the main error in these techniques lies in herbage sampling, and alternative methods which do not require this sampling are being investigated.Using data from indoor digestion experiments, RAYMOND (12) suggested that the composition of faeces produced by grazing stock might be used as an indirect measure of the composition of the herbage grazed. This showed a relationship between the nitrogen contents of herbage and the resulting faeces, % N in ash-free herbage = 0-28 i 0-85 x % N in ash-free faeces [1] and suggested that information on the nutritive value of herbage might be obtained from such relationships. Using similar data, LANCASTER (8, 9) proposed a general relationship between the digestibility of the organic matter in herbage and the nitrogen content of faeces produced:% digestibility of organic matter in herbage = 100(1 \ [2] \ % N in ash-free faeces/ Based on all the data, C ^ 0-76, but more precise estimates were obtained by using C ^ 0-67 for herbage feeds below 15 per cent crude protein and C = 0-80 for feeds above 15 per cent crude protein. HOMB and BRIEREM (4), commenting on this technique, have implied that LANCASTER assumes nitrogen excretion per 100 grm. dry matter intake to be constant, whereas these authors show this to be positively correlated with per cent crude protein in the forage, the correlation depending on the type of forage. However, as noted above, LANCASTER (8) made some allowance for the nitrogen content of the forage. FORBES (2) has proposed an equation taking full account of forage composition: ", , , , ,. , "" /% protein in herbagex % herbage dry matter digested =100 -I \ x \% protein in faeces/ (^100 -42-64 X (% protein in herbage -5%) \ [3] In this however the main advantage of the faecal index method has been lost, in that an estimate of the composition of 'herbage grazed' is required.In connection with their study ofthe use of chromogen as an 'indigestible tracer', REID et al. (16) have noted that an estimate of herbage digestibility can be made without measuring, directly 69 or indirectly, the chromogeti contetit of 'herbage grazed'. They proposed a relationship between herbage digestibility and the chromogen content ofthe resulting faeces: % digestibility of herbage D.M. = O'O168 X + 8-47 log X + 32-74 [4] where X = units chromogen/grm, dry faeces. (Standard error of estimate of digestibility = 0-44%).In a discussion ofthe use of faecal chemical analysis, RAYMOND (13) suggested a study ofthe correlation between herbage digestibility...