The use of inorganic elemental standards in the quantification of proteins and biomolecular compounds by inductively coupled plasma spectrometry

Svantesson, Eva; Pettersson, Jean; Markides, Karin E.

doi:10.1039/b200072p

Cited by 54 publications

(50 citation statements)

References 26 publications

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“…It should be mentioned that an element standard can be used for protein quantification only when the analyte has been identified or the structure and stoichiometry of the analyte has been known. In this case, the matrix interference from the proteins can be negligible and the element standard can be used for protein quantification with an accuracy of 10% or better, as long as the protein concentration is sufficiently low (Svantesson, Pettersson, & Markides, 2002).…”

Section: Standard Curve Methodsmentioning

confidence: 99%

“…All those unique characteristics are propitious for ICP-MS to approach accurate protein quantification via element determination regardless of the diverse matrixes of samples. Such strategy for protein quantification by ICP-MS can achieve high accuracy of 10% or better (Svantesson, Pettersson, & Markides, 2002). Generally, in ICP-MS, the majority of elements in the periodic table can be readily ionized and thus very good detection limits, from pg g À1 to ng g À1 , can be achieved.…”

Section: A Unique Features Of Icp-ms For Protein Quantificationmentioning

confidence: 99%

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ICP‐MS‐Based strategies for protein quantification

Wang

Feng

Zhao

et al. 2009

Mass Spectrometry Reviews

108

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In the post-genomics era, proteomics has become a central branch in life sciences. An understanding of biological functions will not only rely on protein identification, but also on protein quantification in a living organism. Most of the existing methods for quantitative proteomics are based on isotope labeling combined with molecular mass spectrometry. Recently, a remarkable progress that utilizes inductively coupled plasma-mass spectrometry (ICP-MS) as an attractive complement to electrospray MS and MALDI MS for protein quantification, especially for absolute quantification, has been achieved. This review will selectively discuss the recent advances of ICP-MS-based technique, which will be expected to further mature and to become one of the key methods in quantitative proteomics.

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Section: Standard Curve Methodsmentioning

confidence: 99%

Section: A Unique Features Of Icp-ms For Protein Quantificationmentioning

confidence: 99%

ICP‐MS‐Based strategies for protein quantification

Wang

Feng

Zhao

et al. 2009

Mass Spectrometry Reviews

108

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“…2A), the peaks of Ca, Sr, Ba, and Pb, which were selectively contained in dRG-II-B, 5,9-11 coeluted with the B peak at 520 s. The mineral contents of dRG-II-B were determined by calibrating the peak area of the mineral bound to dRG-II-B with that of inorganic element standards. 20 The inorganic standard solution was injected using a postcolumn injector, 21 because free metal ions were often retained on the SE column. Table 1 gives the mineral contents of dRG-II-B from sugar beet and red wine.…”

Section: Mineral Contents Of Drg-ii-bmentioning

confidence: 99%

Characterization of Metal Binding Properties of Rhamnogalacturonan II from Plant Cell Walls by Size-Exclusion HPLC/ICP-MS

Matsunaga

Ishii

2004

ANAL. SCI.

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IntroductionRhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide isolated from vascular plant cell walls, and is composed of a backbone of galacturonic acid (GalA) residues and four side chains of diverse glycosyl residues (Fig. 1). 1,2 RG-II is one of the most complex polysaccharides identified so far; however, its function remained unclear for many years. Recently, a dimeric RG-II-borate complex (dRG-II-B) in which a borate-diol ester (1:2) cross-links two monomeric RG-II (mRG-II) was isolated from pectinase digests of plant cell walls. [3][4][5] It has been shown that RG-II is covalently linked to homogalacturonan (HG), a major component of pectin in plant cell walls, 6 and that dRG-II-B formation contributes to stabilization of the cell walls.7 These studies have demonstrated that the primary function of boron, an essential microelement for plants, is to covalently cross-link wall pectin by dRG-II-B formation, and thereby to contribute to the strength and integrity of the cell walls. 7,8 The dRG-II-B complex contains specific metals, such as Ca, Sr, Ba, and Pb. 5,9-11 The divalent cations (Sr 2+ , Pb 2+ , and Ba 2+ ) with an ionic radius >1.10 Å and trivalent cations (Eu 3+ , Pr 3+ , La 3+ , and Ce 3+ ) with an ionic radius >0.90 Å significantly increased in vitro dRG-II-B formation from mRG-II and boric acid.12 The dRG-II-B with Ca 2+ was likely to stabilize the pectic polysaccharides in the cell walls. 13 Moreover, most of Pb in wine existed as a complex with dRG-II-B;10 Pb bound to the dimer was less available than Pb acetate in rats. 14 However, the function of metal ions bound to dRG-II-B has not been established. In the present study, we characterized the metal binding properties of RG-II using size-exclusion highperformance liquid chromatography/inductively coupled plasma mass spectrometry (SE-HPLC/ICP-MS). 15,16 The metal contents of RG-II and its partial hydrolysates that were treated with metal ions were determined by the SE-HPLC/ICP-MS to understand the RG-II's affinity and binding site for metals. Materials and Methods Preparation of dRG-II-B and mRG-IIdRG-II-B was isolated from sugar beet pulp and red wine, as described previously.4,17 mRG-II was prepared by a 0.1 M HCl treatment of dRG-II-B for 30 min at room temperature. The solution was dialyzed against deionized water and then freezedried. The mineral contents of dRG-II-B were determined by SE-HPLC/ICP-MS using a Diol-120 (8 × 300 mm, YMC Co., Kyoto, Japan) column. Treatment of dRG-II-B and mRG-II with metal ionsSeparate solutions of sugar beet dRG-II-B (0.025 mM) and mRG-II (0.05 mM) in 40 mM ammonium acetate, pH 5.0, containing either CaCl2, SrCl2, BaCl2, Pb(NO3)2, or LaCl3 (0.25 mM) were kept for several hours or more. In a second experiment, solutions of mRG-II (0.025 mM) in 40 mM ammonium acetate, pH 5.0, containing either LaCl3, EuCl3, or LuCl3 (0.25 mM) were prepared. The pH condition (pH 5.0) was selected based on the fact that dRG-II-B is stable above pH 4, 5 and that the apoplastic pH in plants is 5 -6. 18...

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“…The authors also showed the complementary application of capillary LC HR-ICP-MS and capillary LC ESI-MS-MS for the quantification and characterization of tryptic protein digests of two functional domains of the bacterial chemotaxis protein cheAH and cheA-C (257-513) over-expressed in E. coli. 99,179 Over the last few years Heumann and co-workers have promoted the application of isotope-dilution approaches for accurate quantitative elemental speciation analysis using either non-speciesspecific or species-specific strategies. 108,[180][181][182] Based on this development, Prange and Schaumlöffel et al pioneered the field of sulfur isotopedilution analysis by combining postcolumn non-species-specific isotope dilution and capillary electrophoresis hyphenated to sector field ICP-MS for the highly resolved separation of different metallothionein (MT) isoforms, which allowed determination of the metal stoichiometry of the different isoforms as well as their absolute quantification.…”

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confidence: 99%

Inductively Coupled Plasma–Mass Spectrometry (ICP-MS) for Quantitative Analysis in Environmental and Life Sciences: A Review of Challenges, Solutions, and Trends

2012

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This focal point review provides an overview of recent developments and capabilities of inductively coupled plasma mass spectrometry (ICP-MS) coupled with different separation techniques for applications in the fields of quantitative environmental and bio-analysis. Over the past years numerous technical improvements, which are highlighted in this review, have helped to promote the evolution of ICP-MS to one of the most versatile tools for elemental quantification. In particular, the benefits and possibilities of using state-of-the-art hyphenated ICP-MS approaches for quantitative analysis are demonstrated with a focus on environmental and bio-analytical applications.

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The use of inorganic elemental standards in the quantification of proteins and biomolecular compounds by inductively coupled plasma spectrometry

Cited by 54 publications

References 26 publications

ICP‐MS‐Based strategies for protein quantification

ICP‐MS‐Based strategies for protein quantification

Characterization of Metal Binding Properties of Rhamnogalacturonan II from Plant Cell Walls by Size-Exclusion HPLC/ICP-MS

Inductively Coupled Plasma–Mass Spectrometry (ICP-MS) for Quantitative Analysis in Environmental and Life Sciences: A Review of Challenges, Solutions, and Trends

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