The zebrafish miR‐462/miR‐731 cluster is induced under hypoxic stress
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            hypoxia‐inducible factor 1α and functions in cellular adaptations

Huang, Chen; Chen, Nan; Wu, Xianguo; Huang, Chao; He, Yan; Tang, Rong; Wang, Weimin; Wang, Huanling

doi:10.1096/fj.14-267104

Cited by 38 publications

(29 citation statements)

References 79 publications

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“…Mammalian miR-150 is a well-characterised regulator of lymphocyte development, notably Bcell maturation (Baltimore et al, 2008). miR-150 was a hypoxia-induced miRNA in zebrafish larvae (Huang et al, 2015), but its function in teleost antiviral responses is yet to be determined.…”

Section: Discussionmentioning

confidence: 99%

Discovery of microRNAs associated with the antiviral immune response of Atlantic cod macrophages

Eslamloo

Inkpen

Rise

et al. 2018

Molecular Immunology

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MicroRNAs (miRNAs) are known to play important immunoregulatory roles in teleosts, although miRNAs involved in the antiviral immune response of Atlantic cod (Gadus morhua) were previously uncharacterised. Using deep sequencing and qPCR, the present study was conducted to identify miRNAs responsive to the viral mimic, polyriboinosinic polyribocytidylic acid (pIC) in Atlantic cod macrophages. Macrophage samples isolated from Atlantic cod (n=3) and treated with pIC or phosphate buffered saline (PBS control) for 24 and 72h were used for miRNA profiling. Following deep sequencing, DESeq2 analyses identified four (miR-731-3p, miR-125b-3-3p, miR-150-3p and miR-462-3p) and two (miR-2188-3p and miR-462-3p) significantly differentially expressed miRNAs at 24 and 72h post-stimulation (HPS), respectively. Sequencing-identified miRNAs were subjected to qPCR validation using a larger number of biological replicates (n=6) exposed to pIC or PBS over time (i.e. 12, 24, 48 and 72 HPS). As in sequencing, miR-731-3p, miR-462-3p and miR-2188-3p showed significant up-regulation by pIC. The sequencing results were not qPCR-validated for miR-125b-3-3p and miR-150-3p as up- and down-regulated miRNAs at 24 HPS, respectively; however, qPCR results showed significant up-regulation in response to pIC stimulation at later time points (i.e. 48 and/or 72 HPS). We also used qPCR to assess the expression of other miRNAs that were previously shown as immune responsive in other vertebrates. qPCR results at 48 and/or 72 HPS revealed that miR-128-3-5p, miR-214-1-5p and miR-451-3p were induced by pIC, whereas miR-30b-3p and miR-199-1-3p expression were repressed in response to pIC. The present study identified ten pIC-stimulated miRNAs, suggesting them as important in antiviral immune responses of Atlantic cod macrophages. Some pIC-responsive miRNAs identified in this study were predicted to target putative immune-related genes of Atlantic cod (e.g. miR-30b-3p targeting herc4), although the regulatory functions of these miRNAs need to be validated by future studies.

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Section: Discussionmentioning

confidence: 99%

Discovery of microRNAs associated with the antiviral immune response of Atlantic cod macrophages

Eslamloo

Inkpen

Rise

et al. 2018

Molecular Immunology

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“…Sertoli cells with a Dicer-knockout mutation lacked several miRNAs, including miR-125a-3p, while having an overabundance of SOD-1, a protein linked to apoptotic cell death43. In zebrafish embryos, miR-462–5p was also shown to regulate cell proliferation and apoptosis75.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of miRNA transcriptome during gonadal development of zebrafish

Presslauer

Bizuayehu

Kopp

et al. 2017

Sci Rep

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Studies in non-teleost vertebrates have found microRNAs (miRNAs) to be essential for proper gonadal development. However, comparatively little is known about their role during gonadal development in teleost fishes. So far in zebrafish, a model teleost, transcript profiling throughout gonadal development has not been established because of a tiny size of an organ in juvenile stages and its poor distinguishability from surrounding tissues. We performed small RNA sequencing on isolated gonads of See-Thru-Gonad line, from the undifferentiated state at 3 weeks post fertilization (wpf) to fully mature adults at 24 wpf. We identified 520 gonadal mature miRNAs; 111 of them had significant changes in abundance over time, while 50 miRNAs were either testis- or ovary-enriched significantly in at least one developmental stage. We characterized patterns of miRNA abundance over time including isomiR variants. We identified putative germline versus gonadal somatic miRNAs through differential small RNA sequencing of isolated gametes versus the whole gonads. This report is the most comprehensive analysis of the miRNA repertoire in zebrafish gonads during the sexual development to date and provides an important database from which functional studies can be performed.

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“…Moreover, miR-146a could ameliorate liver hypoxia/reoxygenation injury in vitro by directly suppressing IRAK1 and TRAF6 [12] and miR-494 was protective against hypoxia-induced apoptosis in liver through HIF-1α upregulation by activating PI3K/ Akt pathway [14]. In addition, there is accumulating evidence suggesting that many other miRNAs are involved in liver reactions to hypoxic stress, such as miR-150 downregulation under hypoxia by targeting VEGF-A and HIF1α during liver regeneration [16, 57], miR-192-5p upregulation in hepatocyte injury [13] and increased miR-462/731 cluster in the hypoxia-exposed liver of Megalobrama amblycephala [58] and zebrafish [59]. …”

Section: Discussionmentioning

confidence: 99%

Characteristic MicroRNA Expression Induced by δ-Opioid Receptor Activation in the Rat Liver Under Prolonged Hypoxia

Zhi

Shao

Xue

et al. 2017

Cell Physiol Biochem

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Background/Aims: Hypoxic/ischemic injury to the liver is a frequently encountered clinical problem with limited therapeutic options. Since microRNAs (miRNAs) are involved in hypoxic/ ischemic events, and δ-opioid receptor (DOR) is protective against hypoxic/ischemic injury, we asked if pharmacological activation of DOR can alter hypoxic events by regulating miRNA expression in the liver. As the first step, the present work aimed at testing the effect of DOR activation on hepatic miRNA expression in hypoxia. Methods: Male Sprague Dawley rats were exposed to hypoxia (9.5-10% O₂) for 1, 5, or 10 days with or without DOR activation. The target miRNAs were selected according to TaqMan low-density array (TLDA) data and analyzed by quantitative real-time PCR. Results: We found that: 1) 1-day hypoxia caused the upregulation of 9 miRNAs (miR-7a-5p, miR-10a-5p, miR-25-3p, miR-26b-5p, miR-122-5p, miR-128a-3p, miR-135b-5p, miR-145-5p, and miR-181a-5p) and the downregulation of 2 miRNAs (miR-34a-5p and miR-182); 2) 5 and 10-days hypoxia altered the expression of 4 miRNAs (miR-34c-5p, miR-184, miR-107-3p and miR192-5p); 3) DOR activation shifted the expression of 8 miRNAs (miR-122-5p, miR-146a-5p, miR-30e-5p, miR-128a-3p, miR-182, miR-192-5p miR-107-3p and miR-184) in normoxic condition; and 4) DOR activation modified hypoxia-induced changes in 6 miRNAs (miR-142-5p, miR-145-5p, miR-146a-5p, miR-204-5p, miR-34a-5p and miR-192-5p). Conclusion: Hypoxia significantly modifies the miRNA profile in the liver, while DOR activation can modify the hypoxic modification. Therefore, it is potentially possible to alter hypoxic/ischemic pathophysiology in the liver through DOR pharmacotherapy.

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The zebrafish miR‐462/miR‐731 cluster is induced under hypoxic stress via hypoxia‐inducible factor 1α and functions in cellular adaptations

Cited by 38 publications

References 79 publications

Discovery of microRNAs associated with the antiviral immune response of Atlantic cod macrophages

Discovery of microRNAs associated with the antiviral immune response of Atlantic cod macrophages

Dynamics of miRNA transcriptome during gonadal development of zebrafish

Characteristic MicroRNA Expression Induced by δ-Opioid Receptor Activation in the Rat Liver Under Prolonged Hypoxia

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