Therapeutic Effect of Resveratrol on Morphine-Induced Damage in Male Reproductive System of Mice by Reducing Nitric Oxide Serum Level

Abstract

JALILI, C.; SALAHSHOOR, M. R. ; JALILI, F; KAKABARAEI, S.; AKRAMI, A.; SOHRABI, M.; AHOOKHASH, M. & GHANBARI, A.Therapeutic effect of resveratrol on morphine-induced damage in male reproductive system of mice by reducing nitric oxide serum level. Int. J. Morphol., 35(4):1342Morphol., 35(4): -1347Morphol., 35(4): , 2017. SUMMARY:Morphine is one of the naturally occurring phenanthrene alkaloids of opium that induces adverse effects on male reproductive system. Resveratrol is a phytoestrogen and antioxidant of red grape. The main goal is to investigate whether resveratrol could inhibit adverse effects of morphine on sperm cell viability, count, motility as well as testis histology, testosterone hormone and nitric oxide levels in mice. In the present study, 48 male rats were randomly divided into 8 groups (n=6) and were treated intraperitoneally for 14 days with normal saline, resveratrol (2, 8, 20 mg/kg/day), morphine (20 mg/kg/day) and morphine (20 mg/kg/day) + resveratrol (2, 8, 20 mg/kg/day). At the end of experiments, sperm parameters (sperm cell viability, count, motility and morphology), testis weight, the diameter of seminiferous tubules, testosterone hormone level and nitric oxide were analyzed. The data were analyzed by SPSS software for windows (version 20) using one-way ANOVA test followed by Tukey's post hoc test, and P<0.05 was considered significant. The results indicated that morphine administration significantly decreased testosterone level, count, viability and motility of sperm cells and testis weight and increased nitric oxide compared to the saline group (P=0.000). Administration of resveratrol and resveratrol plus morphine significantly increased motility, count and viability of sperm cells, somniferous tubule diameter and testosterone, while it decreased nitric oxide level compared to morphine group (P=0.025). It seems that resveratrol administration could increase the quality of spermatozoa and prevented morphine-induced adverse effects on sperm parameters.

Effects of gallic acid on rat testopathy following morphine administration: an experimental study

“…In the GA and MOR + GA groups, a significant increment was observed in the normal morphology of sperm cells, the STD and testosterone level compared to the MOR control group. MOR can induce an increased level of oxidative stress, DNA damage, peroxidation lipid, as well as formation of additional protein compounds by producing ROS such as superoxide and hydrogen peroxide (8). An increased state of differentiation was reported in seminiferous tubules in the MOR control group in which the increased diameter of the tubules is probable (23).…”

“…Due to low amount of cytoplasmic enzymes, no potential is found for regeneration of oxidative damage. Hence, antioxidants and antioxidant enzymes are highly necessary for the semen fluid to protect against oxidative damage (8). According to the studies by de Oliveira et al GA administration reveals the renal and hepatic anti-oxidative effects on biochemical and histological contents in diabetic mice (28).…”

“…Our results are supported by the findings of Roshankhah et al which showed that administration of crocin might increase SI in diabetic rats (12). ROS can affect the DNA and RNA synthesis in sperm cells (8). Therefore, it was assumed that GA raises the count and SI in the treated groups through improving the anti-oxidant protection of the body (34).…”

“…The prepared slide culture was examined with a magnification of 40×. At least 100 sperm cells were calculated from each random sample from the 10 fields of imagining and the percentage of live sperm cell was documented (8).…”

“…MOR is the main psychoactive substance in the opium family with high potential of addiction, tolerance and psychological dependence (7). MOR in long-term use leads to hypogonadism, hormonal imbalance, and histological changes in male germ cells (8). In a direct effect, MOR has detrimental impacts on spermatogenesis by inhibition of sperm or testicular function, and also impairs the hypothalamic-pituitary testicular axis by an indirect manner, causing male infertility (9).…”