Therapeutic potential of an AcHERV-HPV L1 DNA vaccine

Lee, Hee-Jung; Yoon, Jong Kwang; Heo, Yoonki; Cho, Hansam; Cho, Yeondong; Gwon, Yong-Dae; Kim, Kang Chang; Choi, Junho; Lee, Jae Sung; Oh, Yu‐Kyoung; Kim, Young Bong

doi:10.1007/s12275-015-5150-0

Cited by 7 publications

(6 citation statements)

References 33 publications

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“…8 The secondary structure of the designed L1-L2-E7 (A1, A2) and HSP70-L1-L2-E7 (B1, B2) constructs by PSIPRED server cells [72]. On the other hand, multivalent L1 DNA vaccines could induce strong cellular and humoral immune responses [73,74]. Moreover, chimeric L1 vaccines harboring crossneutralizing L2 peptides (e.g., 17 QLYKTCKQAGTCPPDI-IPKV 36 epitope) were suggested as promising second-generation prophylactic HPV vaccine candidates [75].…”

Section: Discussionmentioning

confidence: 99%

In Silico Design and Immunological Studies of Two Novel Multiepitope DNA-Based Vaccine Candidates Against High-Risk Human Papillomaviruses

et al. 2021

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Human papillomaviruses (HPV)-16 and 18 are the most prevalent types associated with cervical cancer. HPV L1 and L2 capsid proteins and E7 oncoprotein play crucial roles in HPV-related diseases. Hence, these proteins were proposed as target antigens for preventive and therapeutic vaccines. In this study, two multiepitope DNA-based HPV vaccine candidates were designed using in silico analysis including the immunogenic and conserved epitopes of HPV16/18 L1, L2 and E7 proteins (the L1-L2-E7 fusion DNA), and of heat shock protein 70 (HSP70) linked to the L1-L2-E7 DNA construct (the HSP70-L1-L2-E7 fusion DNA). Next, the expression of the L1-L2-E7 and HSP70-L1-L2-E7 multiepitope DNA constructs was evaluated in a mammalian cell line. Finally, immunological responses and antitumor effects of the DNA constructs were investigated in C57BL/6 mice. Our data indicated high expression rates of the designed multiepitope L1-L2-E7 DNA (~ 56.16%) and HSP70-L1-L2-E7 DNA (~ 80.45%) constructs in vitro. The linkage of HSP70 epitopes to the L1-L2-E7 DNA construct significantly increased the gene expression. Moreover, the HSP70-L1-L2-E7 DNA construct could significantly increase immune responses toward Th1 response and CTL activity, and induce stronger antitumor effects in mouse model. Thus, the designed HSP70-L1-L2-E7 DNA construct represents promising results for development of HPV DNA vaccine candidates. KeywordsHuman papillomavirus • Early protein • Late protein • Immunoinformatics tools • Multiepitope DNA vaccine * Azam Bolhassani

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Section: Discussionmentioning

confidence: 99%

In Silico Design and Immunological Studies of Two Novel Multiepitope DNA-Based Vaccine Candidates Against High-Risk Human Papillomaviruses

et al. 2021

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“…A South Korean team showed that an AcHERV-HPV L1 vaccine provided immune-protection for HPV16, 18, and 58. The vaccine also possessed anti-tumour properties to a better extent than that of Cervarix [ 196 ].…”

Section: Treatment Regimensmentioning

confidence: 99%

Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia

Xia

Long

et al. 2021

Cancers

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Human papillomavirus (HPV) infection remains one of the most prominent cancer-causing DNA viruses, contributing to approximately 5% of human cancers. While association between HPV and cervical cancers has been well-established, evidence on the attribution of head and neck cancers (HNC) to HPV have been increasing in recent years. Among the cancer-causing HPV genotypes, HPV16 and 18 remain the major contributors to cancers across the globe. Nonetheless, the distribution of HPV genotypes in ethnically, geographically, and socio-economically diverse East, Southeast, and South Asia may differ from other parts of the world. In this review, we garner and provide updated insight into various aspects of HPV reported in recent years (2015–2021) in these regions. We included: (i) the HPV genotypes detected in normal cancers of the uterine cervix and head and neck, as well as the distribution of the HPV genotypes by geography and age groups; (ii) the laboratory diagnostic methods and treatment regimens used within these regions; and (iii) the oncogenic properties of HPV prototypes and their variants contributing to carcinogenesis. More importantly, we also unveil the similarities and discrepancies between these aspects, the areas lacking study, and the challenges faced in HPV studies.

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“…A recombinant baculovirus that is regulated by both a baculovirus promoter and a mammalian promoter was used to examine the impact of baculovirus infection on genome-wide transcriptome profiles in mammalian cells. In our previous study, we established the recombinant baculovirus AcHERV env expressing the envelope protein of human endogenous retrovirus (HERV) at the viral surface, which enhances virus infectivity in mammalian cells [23][24][25]. HERV env is embedded downstream of the baculovirus polyhedrin (polh) promoter.…”

Section: Generation Of a Dual Promoter-controlled Recombinant Baculov...mentioning

confidence: 99%

Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery

Shin

Choi

Kim

et al. 2020

Genes

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Baculovirus expression systems have been widely used to produce recombinant mammalian proteins owing to the lack of viral replication in vertebrates. Although several lines of evidence have demonstrated impacts of baculovirus infection in mammalian hosts, genome-wide effects have not been fully elucidated. Here, we provide comparative transcriptome profiles of baculovirus and host-immune response genes in recombinant baculovirus-infected mammalian and insect cells. Specifically, to decipher the impacts of baculovirus infection in mammalian cells, we conducted total RNA-seq on human 293TT cells and insect Sf9 cells infected with recombinant baculovirus. We found that baculovirus genes were rarely expressed under the control of baculoviral promoters in 293TT cells. Although some baculovirus early genes, such as PE38 and IE-01, showed limited expression in 293TT cells, baculoviral late genes were mostly silent. We also found modest induction of a small number of mammalian immune response genes associated with Toll-like receptors, cytokine signaling, and complement in baculovirus-infected 293TT cells. These comprehensive transcriptome data will contribute to improving recombinant baculovirus as tools for gene delivery, gene therapy, and vaccine development.

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Therapeutic potential of an AcHERV-HPV L1 DNA vaccine

Cited by 7 publications

References 33 publications

In Silico Design and Immunological Studies of Two Novel Multiepitope DNA-Based Vaccine Candidates Against High-Risk Human Papillomaviruses

In Silico Design and Immunological Studies of Two Novel Multiepitope DNA-Based Vaccine Candidates Against High-Risk Human Papillomaviruses

Current Updates on Cancer-Causing Types of Human Papillomaviruses (HPVs) in East, Southeast, and South Asia

Unraveling the Genome-Wide Impact of Recombinant Baculovirus Infection in Mammalian Cells for Gene Delivery

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