Thidiazuron stimulates adventitious shoot regeneration in different safflower explants

Radhika, K.; Sujatha, M.; Rao, T. Nageshwar

doi:10.1007/s10535-006-0003-7

Cited by 47 publications

(31 citation statements)

References 10 publications

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“…TDZ (Thidiazuron) is a substituted phenyl urea [1-phenyl-3-(1,2,3-thidiazol-5-yl)urea] that has immense potential as a cytokinin in shoot organogenesis in a large number of plant systems including legumes (Murthy et al, 1998;Shan et al, 2000;Ganeshan et al, 2003;Onamu et al, 2003;Mroginski et al, 2004;Sharma et al, 2005;Chen and Chang, 2006;Radhika et al, 2006). Since, the observation made by Malik and Saxena (1992) TDZ has been noted to be the most promising cytokinin for shoot induction in chickpea by number of research teams (Barna and Wakhlu, 1993;Huetteman and Preece, 1993;Murthy et al, 1996;Rizvi and Singh, 2000;Jayanand et al, 2003;Senthil et al, 2004;TewariSingh et al, 2004;Kiran et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

“…Embryonal axis attached to cotyledon showed significant swelling and exhibited initiation of shoot induction within a duration of 6-8 d ( Figure 1C). Use of TDZ with purine ring containing cytokinins such as kinetin, BAP and 2-iP has been shown to promote the formation of a large number of healthy shoots (Eisinger, 1983;Mroginski and Kartha, 1984;Radhika et al, 2006). In order to avoid negative impact of TDZ in the formation of shoots, it was found wise to transfer the explants immediately after shoot induction event to a medium devoid of TDZ.…”

Section: Resultsmentioning

confidence: 99%

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An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Anwar

Sharmila

Saradhi

2008

Physiol Mol Biol Plants

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A rapid, reproducible and efficient regeneration method was developed for chickpea (Cicer arietinum L.) using single cotyledon with half embryonal axis as explants. MS medium supplemented with 4 ìM TDZ, 10 ìM 2-iP and 2 ìM kinetin induced 50-100 adventitious buds/shoots after 14 days of culture and elongated on MS medium supplemented with 5 ìM 2-iP and 2 ìM kinetin. Healthy, strong and 100 % rooting was achieved by exposing cut ends of the shoots to 10 sec pulse treatment with 100 ìmoles/ml IBA followed by their transfer to liquid MS basal medium within 10-14 d. 2-3 cm long shoots were most suitable for rooting. Potting-mixture with good aeration and lesser capacity to retain water was most suitable for achieving successful establishment of chickpea plantlets. Garden soil mixed with sand (gravel) and bio-manure in the ratio of 1:1:1 is most suitable for achieving cent percent transplantation success. Cent percent of plantlets got acclimatized, survived in the pots and showed normal growth, development, flowering followed by podding and seeds setting. Harvesting of seeds was done after the pods were fully matured and dry. In this communication, we have demonstrated for the first time that shoot length, pulse treatment of cut ends of shoots with 100 ìmoles/ml IBA and aeration of potting mixture are key factors for rapid micro-propagation and successful establishment of chickpea.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

Anwar

Sharmila

Saradhi

2008

Physiol Mol Biol Plants

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“…There is a need to develop protocols of tissue culture for large scale multiplication and germplasm maintenance and conservation. The amenability of safflower tissues to in vitro manipulations is evident from simple media requirements for direct and indirect caulogenesis and/or embryogenesis for Indian as well as American cultivars (George and Rao 1982, Ying et al 1992, Orlikowska and Dyer 1993, Mandal et al 1995, 2001, Sujatha and Suganya 1996, Nikam and Shitole 1999, Radhika et al 2006. Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) andnaphtha-leneacetic acid NAA has been frequently used for safflower tissue culture.…”

Section: Safflower (Carthamus Tinctorius L) Of the Familymentioning

confidence: 99%

In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds

Sujatha¹,

Kumar²

2007

Biologia plant.

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The organogenic competence of leaf explants of eleven Carthamus species including C. tinctorius on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ) + -naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) + NAA was investigated. Highly prolific adventitious shoot regeneration was observed in C. tinctorius and C. arborescens on both growth regulator combinations and the shoot regeneration frequency was higher on medium supplemented with TDZ + NAA. Nodal culture of nine Carthamus species on media supplemented with BA and kinetin (KIN) individually revealed the superiority of media supplemented with BA over that of KIN in facilitating a higher shoot proliferation index. Proliferating shoots from axillary buds and leaf explants were transferred to medium supplemented with 1.0 mg dm -3 KIN or 0.5 mg dm -3 BA for shoot elongation. Elongated shoots were rooted on half-strength MS medium supplemented with 1.0 mg dm -3 each of indole-butyric acid (IBA) and phloroglucinol. The plantlets thus obtained were hardened and transferred to soil.

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“…1 It is widely cultivated in agricultural production system of Asia, Europe, Australia and the Americas as a source of high-quality vegetable and industrial oil. 2 Safflower has long been used as a food colorant, dye, and herbal medicine in oriental countries. 3 Safflower edible oil cultivars have the highest quantity of polyunsaturated fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Volatile Constituents in Safflower by Microwave Distillation and Simultaneous Solid‐Phase Microextraction Coupled with Gas Chromatography‐Mass Spectrometry

Yang

Zhou

et al. 2007

Annali di Chimica

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In this paper, microwave distillation and solid-phase microextraction coupled with gas chromatography-mass spectrometry (MD-SPME/GC-MS) was developed for the analysis of essential components in safflower. Using the MD-SPME technique, the isolation, extraction and concentration of volatile compounds in safflower were carried out in only one step. Some parameters affecting the extraction efficiency such as SPME fiber coating, microwave power, irradiation time and the volume of water added were optimized. The optimal experiment parameters obtained were: 65 microm CW/DVB SPME fiber, a microwave power of 400 W, an irradiation time of 3 min and water volume of 1 mL. The proposed method has been compared with conventional steam distillation (SD) for extraction of essential oil compounds in safflower. Using MD-SPME followed by GC-MS, 32 compounds in safflower were separated and identified, which mainly included paeonol, alpha-asarone, beta-asarone, 1-methyl-4-(2-propenyl)-benzene and diethenyl-benzene, whereas only 18 compounds were separated and identified by conventional SD followed by GC-MS. The relative standard deviation (R.S.D.) values of less than 10% show that the proposed method has good reproducibility. The results show that MD-SPME/GC-MS is a simple, rapid, effective method for the analysis of volatile oil components in safflower.

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Thidiazuron stimulates adventitious shoot regeneration in different safflower explants

Cited by 47 publications

References 10 publications

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

An optimal protocol for in vitro regeneration, efficient rooting and stable transplantation of chickpea

In vitro bud regeneration of Carthamus tinctorius and wild Carthamus species from leaf explants and axillary buds

Rapid Determination of Volatile Constituents in Safflower by Microwave Distillation and Simultaneous Solid‐Phase Microextraction Coupled with Gas Chromatography‐Mass Spectrometry

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