Thin-layer bioautographic assay for salinomycin in chicken liver

Dimenna, Gary P.; Walker, Brian E.; Turnbull, Lennox B.; Wright, George J.

doi:10.1021/jf00069a025

Cited by 13 publications

(9 citation statements)

References 2 publications

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“…Consequently, it was not deemed necessary to attempt to purify the antiseium further. The limit of detection of the assay, between 0-1 and 0-2 ng/g, is over 100-fold lower than the thin layer bioautographic assay (Dimenna et al 1986b) which has a limit of detection of 25 ng/g. Similarly, the described assay is more sensitive than the monoclonal antibody preparation which could detect only 500ng/ml in standard solutions (Miller et al 1986).…”

Section: Discussionmentioning

confidence: 86%

Development of an ELISA for salinomycin and depletion kinetics of salinomycin residues in poultry

Kennedy¹,

Blanchflower²,

O'Dornan³

1995

Food Additives and Contaminants

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Salinomycin is widely used as a feed additive to control coccidiosis in the broiler industry world-wide. EC legislation on veterinary drug residues will soon be extended to cover poultry. This will require the development of rapid assays to screen for the presence of residual concentrations of this and other drugs in poultry meat. This study describes the development of an enzyme-linked immunosorbent assay for salinomycin that has a limit of detection of approximately 0.2 ng/g. The antibody cross-reacts with narasin, but is not subject to interference from lasalocid, maduramicin or monensin. Residual concentrations of salinomycin were measured in the tissues of broilers following feeding of medicated feed containing 60 mg/kg salinomycin. Salinomycin residues were present only at very low concentrations in liver and muscle, and fell below the limit of decision of the assay within 2 days of withdrawal of the medicated feed.

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Section: Discussionmentioning

confidence: 86%

Development of an ELISA for salinomycin and depletion kinetics of salinomycin residues in poultry

Kennedy¹,

Blanchflower²,

O'Dornan³

1995

Food Additives and Contaminants

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“…They include thin layer bioautography (Heil et al 1984, Owles 1984, Dimenna et al 1986b, Vanderkop and MacNeil 1990 and liquid chromatography (Goras and Lacourse 1984, Dimenna et al 1986a, 1990, Lapointe and Cohen 1988, Gerhardt et al 1995. The use of enzyme-linked immunosorbentassay (ELISA) has also been explored as an alternative to existing chromatographic techniques for the detection of salinomycin in various biological matrices (Elissalde et al 1993, Muldoon et al 1995.…”

Section: Introductionmentioning

confidence: 99%

Concentrations of salinomycin in eggs and tissues of laying chickens fed medicated feed for 14 days followed by withdrawal for 3 days†

Akhtar

El‐Sooud

Shehata

1996

Food Additives and Contaminants

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Laying chickens were fed medicated feed containing various concentrations of sodium salinomycin (SAL) for 14 days followed by a 3 day withdrawal period. Eggs, collected during treatment and withdrawal, tissues and ovarian yolk of birds slaughtered after 0, 1, and 3 days' withdrawal were extracted and analysed by high performance liquid chromatography (HPLC). Tissues, ovarian yolk and freeze-dried egg albumen and yolk were extracted with acetone, followed by partitioning with petroleum ether and HPLC analysis. Albumen was extracted with methanol and analysed without further clean-up. Salinomycin was detected at 520 nm after post-column reaction with vanillin at 95 degrees C. Recoveries of fortified salinomycin from freeze-dried eggs (albumen and yolk) and tissue, premix and feed were nearly quantitative (> 90%), except liver which was < 85%. The detection limit was estimated to be 5 ng g-1, with the practical quantifiable limit being about 10 ng g-1. Highest SAL concentrations were in the more fatty components such as egg yolk, ovarian yolk and subcutaneous fat. SAL residues in other tissues were generally low and followed the order liver, kidney, thigh and breast muscles. SAL residues were dependent on the SAL concentration in feed and declined rapidly during withdrawal.

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“…[11] Salinomycin and salinomycin sodium (Coxistac Õ ) are registered in Canada for use in meal for broiler chickens [12] at the recommended level of 60 mg kg À1 of complete feed. For example, Dimenna et al [16][17][18] used water to extract SAL residues in liver, skin and fat of chickens and tissues of pigs. [13] The widespread use of SAL in poultry production provides an opportunity for misuse, abuse, and unintentional feeding of medicated feed containing SAL to nonrecommended species, including laying chickens.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Microwave Assisted Extraction with Conventional (Homogenization, Vortexing) for the Determination of Incurred Salinomycin in Chicken Eggs and Tissues

Akhtar¹

2004

J Environ Sci Health B Pest Food Contam Agricul Wastes

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Incurred and fortified salinomycin residues were extracted from chicken tissues and eggs by homogenization, vortexing and by microwave assisted extraction. The salinomycin residues were quantitated by liquid chromatography following postcolumn derivatization with either vanillin or 4-dimcthylamino-benzaldehyde and detected at 520 or 592 nm, respectively. Comparison of residue data indicated that microwave assisted extraction performed as well as the vortexing technique in extracting salinomycin residues in the tissues of laying chickens that were fed meal containing drugs at various level. In the present study, extracts from homogenizing could not be analyzed directly without clean up. Therefore, microwave assisted extraction appears to be a reliable, reproducible, and economical substitute for routinely used homogenization and vortexing extraction techniques.

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Thin-layer bioautographic assay for salinomycin in chicken liver

Cited by 13 publications

References 2 publications

Development of an ELISA for salinomycin and depletion kinetics of salinomycin residues in poultry

Development of an ELISA for salinomycin and depletion kinetics of salinomycin residues in poultry

Concentrations of salinomycin in eggs and tissues of laying chickens fed medicated feed for 14 days followed by withdrawal for 3 days†

Comparison of Microwave Assisted Extraction with Conventional (Homogenization, Vortexing) for the Determination of Incurred Salinomycin in Chicken Eggs and Tissues

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