Thin-layer chromatography of tris(1,10-phenanthroline)iron(II) and tris(2,2′-bipyridine)iron(II) on Sephadex G gels

An Application of X-ray Fluorescence Spectrometry to the Study of Thin-Layer Chromatography

“…Element species could be judged as is. Although we used several commercially available TLC plates, more information about complex formation can be obtained by improving chromatogram by using solid-phase gel filtration …”

“…The subject has not been widely investigated since an idea introduced in 1985 by Toda et al They showed preliminary results for As analysis in a single lane on a TLC plate using XRF coating thickness as a gauge, which was used to measure the thickness of the plated layer. Our report introduced the use of several commercially available TLC plates, but many other stationary phases, such as synthetic polymers, dextran gel, alumina, and silanized silica gel, are also available. Although not, in principle, a chromatographic technique, electrophoresis proves extremely useful in separating charged substances ranging from small ions to large charged macromolecules of biological and biochemical interest.…”

Distribution processes of inorganic solutes in gel chromatography

“…Porath (23) and Ackers (8) have already shown that Kui is a function of the size of the species i (e.g., hydrated radius, jR¡), but to be more exact, Ka of the ionic species should depend not only on the size of the sample ion (Rs) but also on that of the counterion (Rx) because of the requirement of the electrical neutrality (5). Hence, the general expression for Ke,g* can be written Kjx = f(Rsfix) (18) If Ra » Rx, the contribution of Rx to Ka,ax can be neglected Ka,F = /(fis) (19) On the other hand, when Ra « Rx Ka,F = /(fix) (20) F^Kg) are thus given by the following equations corresponding to eq 19 and 20, respectively: Ra » Rx Fx(K5) = log /(Rs) -log /(fix) (21) fis…”

“…APPENDIX II Partition System Involving Ion Association. Between Fe(bpy)32+/or Fe(phen)32+ and halide anion, there exists the ion association equilibrium (18,25) FeL32+ + X" The contributions of the individual processes to the overall partition of the sample ion depend on the corresponding association constants between Sm+ and Xn".…”