Three dimensional study of wounded plant roots recruiting entomopathogenic nematodes with Pluronic gel as a medium

Li, Chunjie; Wang, Yi; Hu, Yanfeng; Cai, Hua; Wang, Congli

doi:10.1016/j.biocontrol.2015.05.007

Cited by 11 publications

(10 citation statements)

References 43 publications

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“…In order to directly examine the foraging behavior of nematodes, Li et al used pluronic gel, a transparent polymer allowing 3D observation of nematodes inside the gel. In accordance with previous results, they found that IJs preferentially aggregated around the wounded parts of roots [ 136 ]. In addition to the release of attractive compounds, roots might also provide nematodes with physical “routeways”, facilitating their progression into the soil [ 137 ].…”

Section: Dispersal and Host Findingsupporting

confidence: 92%

Transmission Success of Entomopathogenic Nematodes Used in Pest Control

Labaude

Griffin

2018

Insects

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Entomopathogenic nematodes from the two genera Steinernema and Heterorhabditis are widely used as biological agents against various insect pests and represent a promising alternative to replace pesticides. Efficacy and biocontrol success can be enhanced through improved understanding of their biology and ecology. Many endogenous and environmental factors influence the survival of nematodes following application, as well as their transmission success to the target species. The aim of this paper is to give an overview of the major topics currently considered to affect transmission success of these biological control agents, including interactions with insects, plants and other members of the soil biota including conspecifics.

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Section: Dispersal and Host Findingsupporting

confidence: 92%

Transmission Success of Entomopathogenic Nematodes Used in Pest Control

Labaude

Griffin

2018

Insects

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“…For the bioassay, the different zones of the Petri dishes were lled with a PF-127 gel solution that contained the nematodes and bacteria in different mixtures. The PF-127 gel was prepared following Li et al (2015). Approximately 25% (wt/vol) Pluronic F-127 gel (NF Prill Poloxamer 407, BASF, Mt Olive, NJ, USA) in 10 mM Tris-MES (morpholino-ethanesulfonic acid) buffer (Sigma-Aldrich) was made and stirred continuously at 4°C overnight.…”

Section: Bioassay To Test For the Effect Of Epns On The Movement Of R...mentioning

confidence: 99%

Direct antagonistic effect of entomopathogenic nematodes and their symbiotic bacteria on root-knot nematodes migration toward tomato roots

Wei

et al. 2022

Plant Soil

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Aims Negative interactions in the rhizosphere between entomopathogenic nematodes (EPNs) and plantparasitic nematodes, such as root-knot nematodes (RKNs), have been documented over the past two decades but the mechanisms and dynamics of such interactions remain largely elusive.Methods Here, we evaluated the effect of inoculation position of two EPN species, Steinernema feltiae and Heterorhabditis bacteriophora, as well as different facets of the EPN-bacterial symbiont complex on the migration of RKNs toward tomato roots, both in sand and in Pluronic gel conditions.Results When EPNs were placed between the position of the RKNs and the roots, the movement of RKNs toward the roots was inhibited. We observed this same pattern both in sand and in Pluronic F-127 (PF-127) gel and for two species of EPNs. We also observed that different components of the EPNs/bacterial symbiont complex (bacteria separate from the nematodes vs. the nematode-bacterium complex), and particularly the cell-free supernatant produced by the bacterial culture, displayed inhibitory effects on RKNs.Conclusions Therefore, the EPNs/bacterial complex, by slowing down the movement of RKNs toward the host plant roots, can function as an effective biocontrol strategy. By screening for the most repulsive strains of EPNs that are also effective against insect pests, such combined target suppression should alleviate EPNs application costs in integrated pest management practices.

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“…Pluronic F-127 (PF127; NF Prill Poloxamer 407, BASF) gel system was used to investigate the interaction of nematode Sc-All with the four insecticides on nematode host-seeking behaviour and infectivity to B. odoriphaga (Wang et al, 2009;Li et al, 2015Li et al, , 2019. Each insecticide at 10% RC was fully mixed with 23% (w/v) PF-127 gel by stirring overnight at 4°C.…”

Section: Host-seeking Behaviour and Infectivity Of Steinernema Carpoc...mentioning

confidence: 99%

Efficacy of entomopathogenic nematodes combined with insecticides against Bradysia odoriphaga larvae

et al. 2022

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Summary Entomopathogenic nematodes (EPN) as an environmentally-friendly biocontrol agent in combination with low toxic insecticides can increase control efficacy against insect pests. In this study, Steinernema carpocapsae All (Sc-All) combined with four common insecticides was used to evaluate the control efficacy against chive root gnat (Bradysia odoriphaga), an important pest of vegetables, e.g., chive, onion or garlic. The compatibility of nematodes with insecticides and host-seeking behaviour were also evaluated by the laboratory bioassay. The results showed three insecticides (matrine, imidacloprid and chlorpyrifos) at the recommended concentrations (RC), 10% RC or 2% RC and insecticide phoxim at 10% RC or 2% RC had no effect on nematodes survival. Sc-All at 50 infective juveniles (IJ) per insect larva in the presence of the four insecticides at 10% RC demonstrated a potentiated, additive or a synergistic effect on the corrected mortality rates of insect up to 100% (imidacloprid) when compared with the corresponding insecticide and Sc-All alone. A synergistic effect resulting in lethal effect was found as early as at 24 h when 200 IJ of Sc-All per insect larva were combined with 10% RC imidacloprid, whilst only 9.4% and 0 corrected mortality were detected, respectively, when exposed to the same amount of imidacloprid and Sc-All alone. For the first time a Pluronic gel system assay revealed that the presence of insecticides significantly improved Sc-All host-seeking ability as early as 30 min post exposure. The results indicated that low doses of Sc-All-imidacloprid combination would be an effective strategy to control chive root gnat.

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Three dimensional study of wounded plant roots recruiting entomopathogenic nematodes with Pluronic gel as a medium

Cited by 11 publications

References 43 publications

Transmission Success of Entomopathogenic Nematodes Used in Pest Control

Transmission Success of Entomopathogenic Nematodes Used in Pest Control

Direct antagonistic effect of entomopathogenic nematodes and their symbiotic bacteria on root-knot nematodes migration toward tomato roots

Efficacy of entomopathogenic nematodes combined with insecticides against Bradysia odoriphaga larvae

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