Three newly delimited species of Saccharomyces sensu stricto

Martini, Alessandro

doi:10.1007/bf00419503

Cited by 213 publications

(118 citation statements)

References 15 publications

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“…On the other hand, the type cultures of S. pastorianus CBS 1538 and S. monacensis CBS 1503 are diploid. These results are in agreement with the DNA-DNA reassociation data (Vaughan-Martini and Martini, 1987). All four S. pastorianus strains have similar DNA reassociation values with the type culture of S. bayanus (72-78%), but differ significantly in DNA relatedness with the type culture of S. cerevisiae.…”

Section: Natural Hybrids Of S Cerevisiae × S Bayanus and S Cerevissupporting

confidence: 90%

“…The data mentioned above are in perfect agreement with the DNA reassociation studies, i.e. strains of both varieties showed high DNA relatedness (86-100%) with the type culture of S. bayanus CBS 380 and low DNA relatedness (3-28%) with the type culture of S. cerevisiae CBS 1171 (Vaughan-Martini and Martini, 1987).…”

Section: Taxonomic Position Of S Bayanus Strains Having Some Subtelosupporting

confidence: 83%

“…Strains CBS 1486and CBS 1513show 54% and 57%, respectively, while CBS 1503and CBS 1538 The results of the present study, along with literature data (Andersen et al, 1999;Yamagishi and Ogata, 1999;Joubert et al, 2000;Casaregola et al, 2001), indicate that S. pastorianus strains are hybrids, but having different genetic constitutions. Judging from the nDNA-nDNA reassociation data (Vaughan-Martini and Martini, 1987), the strains CBS 1486, CBS 1503, CBS 1513 and CBS 1538 contain more complete S. bayanus genome and different fractions of the S. cerevisiae genome. Unlike the S. pastorianus strains isolated from brewing fermentations a century ago, modern lager brewing yeasts contain a complete or almost complete S. cerevisiae genome in addition to the non-S. cerevisiae one (Andersen et al, 1999;Azumi and Goto-Yamamoto, 2001;Casaregola et al, 2001;de Barros Lopes et al, 2002).…”

Section: Natural Hybrids Of S Cerevisiae × S Bayanus and S Cerevismentioning

confidence: 99%

“…The type culture CBS 1513 shows 57% DNA reassociation value with the S. cerevisiae type culture CBS 1171, 72% with the S. bayanus type culture CBS 380 and 93% with the S. pastorianus type culture CBS 1538. Since the latter had been described first, S. pastorianus replaced S. carlsbergensis as the epithet (Vaughan-Martini and Martini, 1987). Initially, S. pastorianus was identified as the wild yeast contaminating the brewing process.…”

Section: Introductionmentioning

confidence: 99%

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Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae

Наумова

Наумов

Masneuf‐Pomarède

et al. 2005

Yeast

121

101

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The genomic constitution of different S. bayanus strains and natural interspecific Saccharomyces hybrids has been studied by genetic and molecular methods. Unlike S. bayanus var. uvarum, some S. bayanus var. bayanus strains (the type culture CBS 380, CBS 378, CBS 425, CBS 1548) harbour a number of S. cerevisiae subtelomeric sequences: Y , pEL50, SUC, RTM and MAL. The two varieties, having 86-100% nDNA-nDNA reassociation, are partly genetically isolated from one another but completely isolated from S. cerevisiae. Genetic and molecular data support the maintaining of var. bayanus and var. uvarum strains in the species S. bayanus. Using Southern hybridization with species-specific molecular markers, RFLP of the MET2 gene and flow cytometry analysis, we showed that the non-S. cerevisiae parents are different in lager brewing yeasts and in wine hybrid strains. Our results suggest that S. pastorianus is a hybrid between S. cerevisiae and S. bayanus var. bayanus, while S. bayanus var. uvarum contributed to the formation of the wine hybrids S6U and CID1. According to the partial sequence of ACT1 gene and flow cytometry analysis, strain CID1 is a triple hybrid between S. cerevisiae, S. kudriavzevii and S. bayanus var. uvarum.

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Section: Natural Hybrids Of S Cerevisiae × S Bayanus and S Cerevissupporting

confidence: 90%

Section: Taxonomic Position Of S Bayanus Strains Having Some Subtelosupporting

confidence: 83%

Section: Natural Hybrids Of S Cerevisiae × S Bayanus and S Cerevismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae

Наумова

Наумов

Masneuf‐Pomarède

et al. 2005

Yeast

121

101

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“…In parallel with the debate over the origin of the brewing yeast hybrid, the composition of the Saccharomyces sensu stricto complex of species is constantly being remodelled (Vaughan-Martini & Martini, 1987 ;Barnett et al, 1990 ;Naumov, 1996 ;Vaughan-Martini & Martini, 1998) as molecular methods increasingly challenge traditional classification (Kurtzman & Robnett, 1998). Three new yeast species were recently added to this group (Naumov et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the constitution of the beer yeast genome by PCR, sequencing and subtelomeric sequence hybridization.

Casarégola¹,

Nguyen²,

Lapathitis³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

102

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The lager brewing yeasts, Saccharomyces pastorianus (synonym Saccharomyces carlsbergensis), are allopolyploid, containing parts of two divergent genomes. Saccharomyces cerevisiae contributed to the formation of these hybrids, although the identity of the other species is still unclear. The presence of alleles specific to S. cerevisiae and S. pastorianus was tested for by PCR/RFLP in brewing yeasts of various origins and in members of the Saccharomyces sensu stricto complex. S. cerevisiae-type alleles of two genes, HIS4 and YCL008c, were identified in another brewing yeast, S. pastorianus CBS 1503 (Saccharomyces monacensis), thought to be the source of the other contributor to the lager hybrid. This is consistent with the hybridization of S. cerevisiae subtelomeric sequences X and Y' to the electrophoretic karyotype of this strain. S. pastorianus CBS 1503 (S. monacensis) is therefore probably not an ancestor of S. pastorianus, but a related hybrid. Saccharomyces bayanus, also thought to be one of the contributors to the lager yeast hybrid, is a heterogeneous taxon containing at least two subgroups, one close to the type strain, CBS 380 T , the other close to CBS 395 (Saccharomyces uvarum). The partial sequences of several genes (HIS4, MET10, URA3) were shown to be identical or very similar (over 99 %) in S. pastorianus CBS 1513 (S. carlsbergensis), S. bayanus CBS 380 T and its close derivatives, showing that S. pastorianus and S. bayanus have a common ancestor. A distinction between two subgroups within S. bayanus was made on the basis of sequence analysis : the subgroup represented by S. bayanus CBS 395 (S. uvarum) has 6-8 % sequence divergence within the genes HIS4, MET10 and MET2 from S. bayanus CBS 380 T , indicating that the two S. bayanus subgroups diverged recently. The detection of specific alleles by PCR/RFLP and hybridization with S. cerevisiae subtelomeric sequences X and Y' to electrophoretic karyotypes of brewing yeasts and related species confirmed our findings and revealed substantial heterogeneity in the genome constitution of Czech brewing yeasts used in production.

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Genomic reorganization between two sibling yeast species,Saccharomyces bayanus andSaccharomyces cerevisiae

Ryu

Murooka

Kaneko

1996

Yeast

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Genomic comparison of two sibling yeast species, Saccharomyces bayanus and Saccharomyces cerevisiae, was performed by Southern blot analysis with various S. cerevisiae gene probes following electrophoretic karyotyping. Fifteen genes on chromosome IV of S. cerevisiae were examined and classified into two groups. Gene probes of CEN4 and TRP1, as well as six other genes located on the left arm of the chromosome hybridized to a 1100‐kb chromosome of S. bayanus that is smaller than chromosome IV of S. cerevisiae. On the other hand, probes of seven genes located on the right arm of chromosome IV hybridized to a 1350‐kb chromosome that is homeologous to chromosome IV, judging from its size. Two genes located on the left arm of chromosome II hybridized to the 1350‐kb chromosome, while four genes on the right arm hybridized to the 1100‐kb chromosome. These pieces of evidence indicate that chromosomes II and IV of S. cerevisiae are rearranged into 1350‐kb and 1100‐kb chromosomes in S. bayanus. Furthermore, it is suggested that chromosome XV is rearranged into two chromosomes (800 and 850 kb in size) in S. bayanus. The translocation points of chromosomes II and IV were delimited using S. cerevisiae prime clone membranes. The results indicated that the translocation points are located close to the FUR4 locus on chromosome II and close to the RAD57 locus on chromosome IV.

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Three newly delimited species of Saccharomyces sensu stricto

Cited by 213 publications

References 15 publications

Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae

Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae

Analysis of the constitution of the beer yeast genome by PCR, sequencing and subtelomeric sequence hybridization.

Genomic reorganization between two sibling yeast species,Saccharomyces bayanus andSaccharomyces cerevisiae

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