Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses

Clewley, Jonathan P.; Gentsch, Jon R.; Bishop, David H. L.

doi:10.1128/jvi.22.2.459-468.1977

Cited by 86 publications

(49 citation statements)

References 27 publications

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“…It was cloned twice before use; stocks were prepared as for BUN. Viruses were assayed by plaque formation on Vero cell monolayers; incubation was for 4 to 5 (10,13). Gels were stained with 0.25% Coomassie blue in methanol-acetic acid-water (50:10:40, vol/vol/ vol), destained in ethanol-acetic acid-water (24:10:66, vol/vol/vol), and fluorographed (2).…”

Section: Methodsmentioning

confidence: 99%

“…Bunyamwera virus (BUN) (20) is the prototype virus of the arbovirus genus Bunyavirus and of the family Bunyaviridae (1,16,19). Members of the Bunyavirus genus contain three size classes of negative-sense, single-stranded RNA: large (L), medium (M), and small (S); each size class contains a unique set of nucleotide sequences (5,18). The S RNA codes for the virion nucleocapsid protein (N) (4,8), and the M RNA codes for the two virion envelope proteins, Gl and G2 (9).…”

mentioning

confidence: 99%

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Seven infection-specific polypeptides in BHK cells infected with Bunyamwera virus

Short¹,

Meek²,

Dalgarno³

1982

J Virol

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Virus-specific polypeptide synthesis was examined in BHK cells and Vero cells infected with Bunyamwera virus. In BHK cells, in addition to the four previously reported virus-coded proteins (L, G1, G2, and N), three other infection-specific proteins were detected. These proteins, of nominal molecular weight 50,000 (p50), 16,000 (p16), and 13,000 (p13), were not labeled in mock-infected cells, were first synthesized between 4 and 8 h after infection, and were relatively prominent among the limited number of proteins generated late in infection. In preparations of purified Bunyamwera virus from BHK cell supernatants, p16 was detected but not p50 or p13. In Vero cells infected with Bunyamwera virus, both p50 and p13 were labeled strongly. Maprik virus, a member of the Mapputta group of arboviruses, is a member of the Bunyavirus genus (S.E. Newton, unpublished data). Maprik virus did not induce the synthesis of p50, p16, or p13; however, two smaller proteins (p17 and p15) which may correspond to p16 and p13 were labeled late in Maprik infection. Our data argue that p16 is a virus-coded component of the Bunyamwera virus particle and that p50 and p13 are virus-coded, nonstructural proteins.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Seven infection-specific polypeptides in BHK cells infected with Bunyamwera virus

Short¹,

Meek²,

Dalgarno³

1982

J Virol

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“…I~NA was precipitated at --2 0°C for 16 hours by adding 2 volumes of 100 per cent ethanol and 0.1 volume of 2 ~ sodium acetate. R N A was digested to completion with l~NAse T 1 and electrophoresed in two dimensions on polyacrylamide gels according to the method of DEW~CH~E~ and FERS (5) as modified by CLEWLEY et al (3).…”

Section: Analysis O / R N Amentioning

confidence: 99%

Oligonucleotide fingerprints of antigenically related bovine coronavirus and human coronavirus OC43

Lapps

Brian

1985

Archives of Virology

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Virion RNAs from the bovine enteric coronavirus and the human respiratory coronavirus OC43 were compared by one dimensional gel electrophoresis and by oligonucleotide fingerprinting. For each virus, approximately 55 per cent of the RNA migrated as a 6.8 Md species, 10 per cent as a 0.68 Md species, and 15 per cent as heterogeneous small molecular weight RNA. A sequence homology of greater than 96 per cent was observed between the 6.8 Md species from the two viruses. The 0.68 Md RNA is apparently an intravirion, subgenomic, polyadenylated molecule based on RNAse studies, oligo (dT)-cellulose chromatography, and hybridization to a cDNA clone of the 3' terminal 1.19 Kb region of the bovine coronavirus genome.

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“…The Bunyaviridae is a family of negativestranded, enveloped RNA viruses which have genomes consisting of three single-stranded RNA species, designated L, M, and S (6,8,12,13). Analyses of Lumbo virus, a variety of Tahyna virus, which is a member of the California encephalitis serogroup of bunyaviruses (13), have established that Lumbo virus preparations have a virion RNA-directed RNA polymerase (2) and that viral complementary RNA (cRNA) similar in size to the virion RNA species can be isolated from Lumbo virus-infected cell polysomes (4).…”

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confidence: 99%

Genome complexities of the three mRNA species of snowshoe hare bunyavirus and in vitro translation of S mRNA to viral N polypeptide

Cash¹,

Vezza²,

Gentsch³

et al. 1979

J Virol

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The genome complexities of the principal intracellular viral complementary RNA species of the snowshoe hare bunyavirus have been analyzed by duplex analyses involving hybridization of complementary RNA to individual 32P-labeled viral RNA species (large, L; medium, M; and small, S), recovery of nucleaseresistant duplexes, and determination of the oligonucleotide fingerprints of the protected 32P-labeled viral sequences. The result for the M RNA (which codes for the glycoproteins Gl and G2; J. R. Gentsch and D. H. L. Bishop, J. Virol. 30:767-770, 1979) indicates that there is a single polycistronic M mRNA. Similar results were obtained for the L and S RNA species. In vitro translation studies with the S complementary RNA species of snowshoe hare virus as well as melted purified S duplexes substantiate earlier genetic and molecular studies (

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Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses

Cited by 86 publications

References 27 publications

Seven infection-specific polypeptides in BHK cells infected with Bunyamwera virus

Seven infection-specific polypeptides in BHK cells infected with Bunyamwera virus

Oligonucleotide fingerprints of antigenically related bovine coronavirus and human coronavirus OC43

Genome complexities of the three mRNA species of snowshoe hare bunyavirus and in vitro translation of S mRNA to viral N polypeptide

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