Tissue-Engineered Human Vascular Media Produced<i>in Vitro</i>by the Self-Assembly Approach Present Functional Properties Similar to Those of Their Native Blood Vessels

Laflamme, Karina; Roberge, Claude; Pouliot, Sté Phanie; D’Orléans-Juste, Pedro; Auger, Franç Ois A.; Germain, Lucie

doi:10.1089/ten.2006.12.ft-179

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…En chirurgie digestive (traitement des fistules dans la maladie de Crohn) [186], des essais cliniques sont en cours, et leur utilisation dans les années à venir en cardiologie, hépatologie et orthopédie est envisageable [179]. [188,189] aux propriétés mécaniques proches des vaisseaux natifs [190], possédants des propriétés vasoactives [191] et la mise au point de substituts cornéens et urologiques [192,193]. La cellularisation préalable d'une matrice (naturelle ou synthétique) présente des intérêts bien connus.…”

Section: Matériels Et Méthodesunclassified

Esophageal tissue engineering

Luc

Durand

Collet

et al. 2014

Expert Review of Medical Devices

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Decellularized matrixes (DM) are commonly used to facilitate a constructive remodeling response in several types of tissue, including the esophagus. Surgical procedure of the esophagus is often complicated by stricture, but preclinical studies have shown that the use of a DM can mitigate stricture and promote a constructive outcome.Recognizing the potential benefits of DM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo remodeling properties of DM from porcine esophagus.The developed protocol for esophageal DM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a good mechanical behavior. Stem cells remained viable when seeded upon the esophageal DM in vitro, and the in-vivo host response showed a pattern of constructive remodeling when implanted in soft tissue.

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Section: Matériels Et Méthodesunclassified

Esophageal tissue engineering

Luc

Durand

Collet

et al. 2014

Expert Review of Medical Devices

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“…Interestingly, TEBVs constructed by self-assembly (SA-TEBV) have shown superior mechanical strength (burst pressure more than 1000 mmHg), morphology, structural organization and vasomotor response compared with other TEBVs [2,[10][11][12][13][14][15][16]. The self-assembly approach consists of culturing human umbilical smooth muscle cells (hUSMCs) and dermal fibroblasts (hDFs) in supplemented culture media; after several weeks the cells form a thick living tissue sheet that can be rolled around a mandrel and cultured to form a TEBV with layers similar to the media and adventitia of native vessels.…”

Section: Introductionmentioning

confidence: 99%

Biaxial biomechanical properties of self-assembly tissue-engineered blood vessels

Zaucha

Gauvin

Auger

et al. 2010

J. R. Soc. Interface.

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Along with insights into the potential for graft success, knowledge of biomechanical properties of small diameter tissue-engineered blood vessel (TEBV) will enable designers to tailor the vessels' mechanical response to closer resemble that of native tissue. Composed of two layers that closely mimic the native media and adventitia, a tissue-engineered vascular adventitia (TEVA) is wrapped around a tissue-engineered vascular media (TEVM) to produce a self-assembled tissue-engineered media/adventia (TEVMA). The current study was undertaken to characterize the biaxial biomechanical properties of TEVM, TEVA and TEVMA under physiological pressures as well as characterize the stress-free reference configuration. It was shown that the TEVA had the greatest compliance over the physiological loading range while the TEVM had the lowest compliance. As expected, compliance of the SA-TEBV fell in between with an average compliance of 2.73 MPa 21 . Data were used to identify material parameters for a microstructurally motivated constitutive model. Identified material parameters for the TEVA and TEVM provided a good fit to experimental data with an average coefficient of determination of 0.918 and 0.868, respectively. These material parameters were used to develop a two-layer predictive model for the response of a TEVMA which fit well with experimental data.

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