Tissue response to nano-hydroxyapatite/collagen composite implants in marrow cavity

Du, Chang; Cui, Fu Zhai; Feng, Qingling; Zhu, Xun; Groot, K. de

doi:10.1002/(sici)1097-4636(19981215)42:4<540::aid-jbm9>3.0.co;2-2

Cited by 235 publications

(83 citation statements)

References 34 publications

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“…Already in 1990-s, implants prepared from nanodimensional apatites, as well as biocomposites of nanodimensional apatite with organic compounds were tested in vivo [606][607][608]. Cylinders made of both pure nanodimensional apatite and organoapatite containing a synthetic peptide were analyzed 28 days after implantation into spongy bones of Chinchilla rabbits.…”

Section: Bone Repairsupporting

confidence: 90%

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Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

AJBE

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Nano-sized particles and crystals play an important role in the formation of calcified tissues of various animals. For example, nano-sized and nanocrystalline calcium orthophosphates in the form of apatites of biological origin represent the basic inorganic building blocks of bones and teeth of mammals. Namely, according the recent developments in biomineralization, tens to hundreds nanodimensional crystals of a biological apatite are self-assembled into these complex structures. This process occurs under a strict control by bioorganic matrixes. Furthermore, both a greater viability and a better proliferation of various types of cells have been detected on smaller crystals of calcium orthophosphates. Thus, the nano-sized and nanocrystalline forms of calcium orthophosphates have a great potential to revolutionize the hard tissue-engineering field, starting from bone repair and augmentation to controlled drug delivery systems. This review reports on current state of the art and recent developments on the subject, starting from synthesis and characterization to biomedical and clinical applications. Furthermore, the review also discusses possible directions for future research and development.

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Section: Bone Repairsupporting

confidence: 90%

“…That study revealed a suitability of such materials for both bone replacement and drug release purposes [606]. Similar results were obtained in other studies [607,608].…”

Section: Bone Repairmentioning

confidence: 99%

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Dorozhkin¹

2012

AJBE

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“…However, it is very brittle and cannot be applied to the load-bearing site directly [3][4][5]. To overcome these limitations, HA has been incorporated with natural biomacromolecules such as collagen [6][7][8] and gelatin [9,10], or synthetic polymers such as poly (α-hydroxyl acids) [11][12][13][14][15], poly (ε-caprolactone) (PCL) [16,17], polyamide [18], and polymethylmethacrylate (PMMA) [19] to prepare composites using a variety of methods including surface coating, grafting, direct mixing, and biomimetic precipitation [10,11,[20][21][22][23]. Particularly, polymer/HA nanocomposites have improved mechanical properties and enhanced cell attachment, spreading, and proliferation on their surfaces by adding nano-sized HA to modify the polymer's characteristics and/or strengthen the polymer matrix [24,25].…”

Section: Introductionmentioning

confidence: 99%

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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A series of crosslinkable nanocomposites has been developed using hydroxyapatite (HA) nanoparticles and poly(propylene fumarate) (PPF). PPF/HA nanocomposites with four different weight fractions of HA nanoparticles have been characterized in terms of thermal and mechanical properties. To assess surface chemistry of crosslinked PPF/HA nanocomposites, their hydrophilicity and capability of adsorbing proteins have been determined using static contact angle measurement and MicroBCA protein assay kit after incubation with 10% fetal bovine serum (FBS), respectively. In vitro cell studies have been performed using MC3T3-E1 mouse pre-osteoblast cells to investigate the ability of PPF/HA nanocomposites to support cell attachment, spreading, and proliferation after 1, 4, and 7 days. By adding HA nanoparticles to PPF, the mechanical properties of crosslinked PPF/ HA nanocomposites have not been increased due to the initially high modulus of crosslinked PPF. However, hydrophilicity and serum protein adsorption on the surface of nanocomposites have been significantly increased, resulting in enhanced cell attachment, spreading, and proliferation after 4 days of cell seeding. These results indicate that crosslinkable PPF/HA nanocomposites are useful for hard tissue replacement because of excellent mechanical strength and osteoconductivity.

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“…If properly designed, a composite should result in an implant exhibiting bioactivity and tailored physical, biological and mechanical properties for specific biomedical applications (Li & Chang 2005a,b). To date, various biocomposites of biodegradable polymers, bioceramics and bioactive glasses have been used as bone generation materials with varying degrees of success (Du et al 1997;Roether et al 2002). Recently, researchers in the field of orthopaedics have placed considerable emphasis on developing bioactive composites of nano-ceramics and polymers (wherein nano-bioactive material particles are dispersed in suitable polymeric matrices) owing to their bone analogue design as well as their biological performance (Murugan & Ramakrishna 2005).…”

Section: Introductionmentioning

confidence: 99%

Comparison of physical, chemical and cellular responses to nano- and micro-sized calcium silicate/poly(ε-caprolactone) bioactive composites

Wei

Heo

Kim

et al. 2007

J. R. Soc. Interface.

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In this study, we fabricated nano-sized calcium silicate/poly(3-caprolactone) composite (n-CPC) and micro-sized calcium silicate/poly(3-caprolactone) composite (m-CPC). The composition, mechanical properties, hydrophilicity and degradability of both n-CPC and m-CPC were determined, and in vitro bioactivity was evaluated by investigating apatite forming on their surfaces in simulated body fluid (SBF). In addition, cell responses to the two kinds of composites were comparably investigated. The results indicated that n-CPC has superior hydrophilicity, compressive strength and elastic modulus properties compared with m-CPC. Both n-CPC and m-CPC exhibited good in vitro bioactivity, with different morphologies of apatite formation on their surfaces. The apatite layer on n-CPC was more homogeneous and compact than on m-CPC, due to the elevated levels of calcium and silicon concentrations in SBF from n-CPC throughout the 14-day soaking period. Significantly higher levels of attachment and proliferation of MG63 cells were observed on n-CPC than on m-CPC, and significantly higher levels of alkaline phosphatase activity were observed in human mesenchymal stem cells (hMSCs) on n-CPC than on m-CPC after 7 days. Scanning electron microscopy observations revealed that hMSCs were in intimate contact with both n-CPC and m-CPC surfaces, and significantly cell adhesion, spread and growth were observed on n-CPC and m-CPC. These results indicated that both n-CPC and m-CPC have the ability to support cell attachment, growth, proliferation and differentiation, and also yield good bioactivity and biocompatibility.

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Tissue response to nano-hydroxyapatite/collagen composite implants in marrow cavity

Cited by 235 publications

References 34 publications

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Nanodimensional and Nanocrystalline Calcium Orthophosphates

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Comparison of physical, chemical and cellular responses to nano- and micro-sized calcium silicate/poly(ε-caprolactone) bioactive composites

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