Titanium Surface Coating with a Laminin-Derived Functional Peptide Promotes Bone Cell Adhesion

Abstract

Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy.

Osseoconductivity of a Specific Streptavidin–Biotin–Fibronectin Surface Coating of Biotinylated Titanium Implants – A Rabbit Animal Study

“…When the shear physical adsorption of suitable biomolecules is not sufficient, stability and release of the coating factors are not controlled . Therefore, covalent coupling of growth factors to titanium surfaces has been in the focus of recent research . Fibronectin is an extracellular matrix protein that affects the osteogenic response directly by integrin‐mediated signaling pathways and is of high relevance to acceleration of early phases of osteogenesis .…”

“…25,42 Therefore, covalent coupling of growth factors to titanium surfaces has been in the focus of recent research. 15,[25][26][27][43][44][45] Fibronectin is an extracellular matrix protein that affects the osteogenic response directly by integrin-mediated signaling pathways 43 and is of high relevance to acceleration of early phases of osteogenesis. 46 We have already shown that the biofunctional coating of biotinylated fibronectin on titanium surfaces via the novel streptavidin-biotin system delivers reliable and promising in vitro results.…”

Strategies For Immobilization Of Bioactive Organic Molecules On Titanium Implant Surfaces – A Review

“…Finally, Min et al investigated the attachment and osteoblastic gene expression of osteoblast-like cells seeded onto a laminin-derived functional peptide (Ln2-P3) coated Ti implant surface using the human osteosarcoma (HOS) cell line. 7 Ln2-P3 is a laminin-derived functional peptide having the DLTIDDSYWYRI sequence. It was deposited on three different SLA (sandblasted with large grit and acid etched) surfaces, anodized Ti surface and calcium phosphorus (Ca-P) coated titanium surface.…”

Fabrication of a biomimetic ZeinPDA nanofibrous scaffold impregnated with BMP‐2 peptide conjugated TiO 2 nanoparticle for bone tissue engineering

“…The smaller size and higher diffusivity of this peptide entailed for further immobilization onto the nanoparticles (NPs) that induces rapid internalization and prolong the retention time of biomolecules at the target site (Singh & Lillard, ). Among the functional nanoparticles, the immobilization of biomolecules on TiO 2 nanoparticles improved bone cell adhesion (Min et al, ), biomechanical stability, and osseointegration without any signs of inflammation (Karlsson et al, ). The positively charged TiO 2 nanoparticle thin film surface promotes cell adhesion of mesenchymal stem cells (Kommireddy, Sriram, Lvov, & Mills, ) and induced cellular proliferation and differentiation into osteoblast‐like cells in human mesenchymal stem cells (Oh et al, ).…”